Vaccination with human papillomavirus type 16-derived peptides using a tattoo device

Pokorná, Dana; Poláková, Ingrid; Kindlová, Martina; Dušková, Martina; Ludvı́ková, Viera; Gabriel, Pavel; Kutinovà, L; Müller, Martin; Šmahel, Michal

doi:10.1016/j.vaccine.2009.03.073

Cited by 25 publications

(17 citation statements)

References 32 publications

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“…All classes can induce potent Th1 effects for a variety of antigens [90][91][92][93][94][95][96]. In reality, use of CpGs often generates a balanced and more effective im-mune response.…”

Section: Oligonucleotidesmentioning

confidence: 99%

Selection of Adjuvants for Enhanced Vaccine Potency

Wang¹,

Singh²

2011

WJV

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“…All classes can induce potent Th1 effects for a variety of antigens [90][91][92][93][94][95][96]. In reality, use of CpGs often generates a balanced and more effective im-mune response.…”

Section: Oligonucleotidesmentioning

confidence: 99%

Selection of Adjuvants for Enhanced Vaccine Potency

Wang¹,

Singh²

2011

WJV

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“…Finally, DNA tattooing can cover a large area of skin, which could potentially elicit a stronger immune response. Tattooing devices can also be used to deliver peptide/protein vaccines, and have been proven to induce both humoral and cell-mediated immune responses 10 . We now use skin tattooing routinely in our animal experiments in a DNA prime-protein boost vaccination protocol (three DNA primes followed by two protein boosts over a period of 16 weeks), and we have successfully induced in mice strong immune responses against HIV-1 gp120.…”

Section: Discussionmentioning

confidence: 99%

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

Chiu

Sampson

Jiang

et al. 2012

JoVE

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Nucleic acid-based vaccination is a topic of growing interest, especially plasmid DNA (pDNA) encoding immunologically important antigens. After the engineered pDNA is administered to the vaccines, it is transcribed and translated into immunogen proteins that can elicit responses from the immune system. Many ways of delivering DNA vaccines have been investigated; however each delivery route has its own advantages and pitfalls. Skin tattooing is a novel technique that is safe, cost-effective, and convenient. In addition, the punctures inflicted by the needle could also serve as a potent adjuvant. Here, we a) demonstrate the intradermal delivery of plasmid DNA encoding enhanced green fluorescent protein (pCX-EGFP) in a mouse model using a tattooing device and b) confirm the effective expression of EGFP in the skin cells using confocal microscopy. Video LinkThe video component of this article can be found at

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“…The tattoo delivery of DNA has been found as a cost-effective method that may be used in laboratory conditions when more rapid and more robust immune responses are required (Pokorná et al, 2009). The second method, a novel dry-coated densely packed Macroflux® microprojections array skin patch, was established as an alternate delivery system to intramuscular injection for delivering an alum adjuvanted vaccine Gardasil (Shi et al, 2005).…”

Section: Hpv Vaccines Of the First Generation The Need For Second-gementioning

confidence: 99%