Vaccination with a single dose of a recombinant porcine adenovirus expressing the classical swine fever virus gp55 (E2) gene protects pigs against classical swine fever

Hammond, J. M.; McCoy, Richard J.; Jansen, Elisa S.; Morrissy, Chris; Hodgson, A. L. M.; Johnson, Michael A.

doi:10.1016/s0264-410x(99)00347-3

Cited by 69 publications

(47 citation statements)

References 32 publications

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“…Sporadic temporary increases of physiological body temperature after CSFV challenge infection was observed in this experiment but also occur after vaccination with E2-DNA vaccine [1] and after vaccination using a recombinant E2-Adenovirus [17].…”

Section: Discussionsupporting

confidence: 56%

“…In addition, the fourth animal who had not developed neutralizing antibodies also survived similar to the described phenomenon after live vaccination [9]. Although T lymphocytes seem to be involved in the development of a protective immune response against CSFV [2,31] the presence of even low titer neutralizing antibodies is a good prognostic marker [17,33]. Discussing the Th1/Th2 paradigma described for other species as for swine, our results suggest that the expression of IL-12 in conjunction with the E2 glycoprotein prevented the occurrence of CSFV neutralizing serum antibodies [37,38] probably by a predominance of cellular response, e.g.…”

Section: Discussionmentioning

confidence: 53%

“…Marker vaccines based on recombinant glycoprotein E2 allow discrimination between vaccinated and infected animals. Several groups working on CSFV vaccines have shown that especially the E2-protein is able to induce a specific and protective immune response against CSFV after vaccination with an E2-expressing recombinant virus [17,22,32], by immunization with baculovirus expressed E2 protein [5,44,45]. These experiments have demonstrated that glycoprotein E2 is the most immunogenic and protective protein of CSFV inducing e.g.…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

et al. 2005

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-The aim of this study was to determine the immunomodulatory effects of IL-12, IL-18 and CD154 (CD40 ligand, CD40L) in DNA-vaccination against the classical swine fever virus. Four recombinant plasmids were constructed including the CSFV coding region for the glycoprotein gp55/E2 alone or together with porcine IL-12, IL-18 or CD154 genes. Five groups of four pigs each were immunized intramuscularly (i.m.) three times with the respective constructs. The control group was inoculated with empty plasmid DNA. Eighteen days after the final immunization, the pigs were challenged with a lethal dose of CSFV strain Eystrup and monitored for a further 16 days. This study showed that co-delivery of IL-18 and CD154 induced an earlier appearance of serum antibodies, reduced B-cell deficiency after infection and protected pigs against a lethal CSFV infection. In contrast, co-delivery of IL-12 led to a reduced titer of neutralizing antibodies and protection against a lethal CSFV challenge in comparison to the other pigs and to pigs that were immunized with a gp55/E2 plasmid alone.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

et al. 2005

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“…Only ORFs located at the ends of the CELO genome were mutated, i.e, the 16 ORFs located on the A fragment (ORFs 1, 2, 3, 4, 12, 13, 14, and 15) and the D fragment (ORFs 7,8,9,10,11,16,17,and 18) (Fig. 3).…”

Section: Methodsmentioning

confidence: 99%

“…Human adenoviruses (type 2 and 5) have been for some time developed as gene delivery vectors for vaccination or gene therapy (2,9,17,24,35,36) and more recently, adenoviruses from various species (bovine, ovine, porcine, canine, and avian) have been studied for similar applications (11,16,23,25,28,29,30,32). Among avian adenoviruses, which include at least 10 serotypes (3,4,8,12,27,32), chicken embryo lethal orphan (CELO), which represents serotype 1, is the best characterized (6,7,19,20,22,23,26).…”

mentioning

confidence: 99%

Construction of Avian Adenovirus CELO Recombinants in Cosmids

François¹,

Eterradossi

Delmas

et al. 2001

J Virol

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The avian adenovirus CELO is a promising vector for gene transfer applications. In order to study this potentiality, we developed an improved method for construction of adenovirus vectors in cosmids that was used to engineer the CELO genome. For all the recombinant viruses constructed by this method, the ability to produce infectious particles and the stability of the genome were evaluated in a chicken hepatocarcinoma cell line (LMH cell line). Our aim was to develop a replication-competent vector for vaccination of chickens, so we first generated knockout point mutations into 16 of the 22 unassigned CELO open reading frames (ORFs) to determine if they were essential for virus replication. As the 16 independent mutant viruses replicated in our cellular system, we constructed CELO genomes with various deletions in the regions of these nonessential ORFs. An expression cassette coding for the enhanced green fluorescent protein (eGFP) was inserted in place of these deletions to easily follow expression of the transgene and propagation of the vector in cell monolayers. Height-distinct GFP-expressing CELO vectors were produced that were all replication competent in our system. We then retained the vector backbone with the largest deletion (i.e., 3.6 kb) for the construction of vectors carrying cDNA encoding infectious bursal disease virus proteins. These CELO vectors could be useful for vaccination in the chicken species.Members of the family Adenoviridae are nonenveloped viruses with double-stranded linear DNA genomes that are 25 to 45 kb in length (13). Human adenoviruses (type 2 and 5) have been for some time developed as gene delivery vectors for vaccination or gene therapy (2,9,17,24,35,36) and more recently, adenoviruses from various species (bovine, ovine, porcine, canine, and avian) have been studied for similar applications (11,16,23,25,28,29,30,32). Among avian adenoviruses, which include at least 10 serotypes (3,4,8,12,27,32), chicken embryo lethal orphan (CELO), which represents serotype 1, is the best characterized (6,7,19,20,22,23,26). The CELO virus genome is 43,804 bp long and has been completely sequenced (7), and its transcriptional organization has been established (26). The central region of the viral genome is strongly homologous with other adenoviruses: the lower strand encodes replication functions (DNA polymerase, DNA-binding protein, pTP), and the upper strand, which is transcribed under the control of a single major late promoter, encodes capsid structural proteins. On either side of this central part there are two regions encoding at least 22 unassigned open reading frames (ORFs) that have no sequence homology with the E1, E3, and E4 regions of mammalian adenoviruses (mastadenoviruses). Only 2 of these 22 genes have been studied: ORF8 encodes GAM-1 protein, which was identified as a functional homolog to human adenovirus E1B 19K protein (6), and ORF22 encodes a protein that interacts with the retinoblastoma protein, which is similar to human adenovirus E1A protein, and cooperates with GAM-1 ...

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2009

EFSA Journal

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BACKGROUND AS PROVIDED BY THE COMMISSION Classical swine fever (CSF) is one of the diseases that has caused major socioeconomic damages in the EU during the last decades. Although during the last years considerable progress has been made in the eradication and prevention of the disease, the threat for an epidemic still exists. The main reasons are that CSF virus is still present in feral pigs of some Member States (MSs) and that the virus is endemic in the Balkan region, including the MSs Bulgaria and Romania. Control measures are in place for those areas within the EU but this situation remains a constant threat for new outbreaks in the domestic pig population.

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Vaccination with a single dose of a recombinant porcine adenovirus expressing the classical swine fever virus gp55 (E2) gene protects pigs against classical swine fever

Cited by 69 publications

References 32 publications

Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination

Construction of Avian Adenovirus CELO Recombinants in Cosmids

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