Vaccination with a Plasmid Vector Carrying the Rabies Virus Glycoprotein Gene Induces Protective Immunity against Rabies Virus

Xiang, Zhi Quan; Spitalnik, Steven L.; Tran, Minh H; Wunner, William H.; Cheng, Jun; Ertl, Hildegund C. J.

doi:10.1006/viro.1994.1105

Cited by 364 publications

(155 citation statements)

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“…Those immunized once showed low CFA/Ispecific antibody levels (titer of log 10 2, average of positive animals) but a second DNA dose elicited a systemic CFA/I-specific antibody response in most animals, although with a rather larger titer range (average of log 10 5.2 ± 2 in IgG-ELISA) (data not shown). Similar results were also observed with other DNA vaccines, in which more than one dose was required for full induction of an antibody response, probably due to the reduced number of transfected cells (2,4,5). In contrast to the antibodies induced by pRECFA, serum antibodies raised against pBLCFAencoded CFA/I inhibited the binding properties of intact CFA/I fimbriae (Table 1).…”

Section: Dna Vaccines Against Enterotoxigenic E Colisupporting

confidence: 46%

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New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin

Alves

Lásaro

Almeida

et al. 1999

Braz J Med Biol Res

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Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen I (CFA/I), an ETEC fimbrial adhesin, were constructed. Eukaryotic cells transfected with each of these plasmids expressed the heterologous antigen in different compartments: bound to the cytoplasmic membrane (pRECFA), accumulated in the cytoplasm (pPolyCFA) or secreted to the outside medium (pBLCFA). BALB/c mice were intramuscularly (im) inoculated with purified plasmid DNA and the systemic, cellular and secreted CFA/I-specific immune responses were analyzed. The results showed that all three DNA vaccine formulations could elicit CFA/I-specific immune responses. Moreover, cellular location of the plasmid-encoded CFA/I seems to have an important role in the induced immune response. Taken together, these results indicate that DNA vaccines also represent a promising approach against enteric bacterial pathogens.

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Section: Dna Vaccines Against Enterotoxigenic E Colisupporting

confidence: 46%

“…DNA vaccines also represent a useful technique to study the antibody responses against a large number of virus-, parasite-and bacterium-derived antigens (2)(3)(4)(5)(6). Nonetheless, development of DNA vaccines against enteric bacterial pathogens has not been reported.…”

Section: Introductionmentioning

confidence: 99%

New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin

Alves

Lásaro

Almeida

et al. 1999

Braz J Med Biol Res

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“…vaccination with plasmid DNA (pDNA) results in protective immunity in many species against viral, [5][6][7][8][9][10][11] bacterial, [12][13][14] and parasitic diseases, 15 including malaria. 16 Toward the goal of developing an effective genetic malaria vaccine, a phase I trial was recently completed in healthy volunteers using a pDNA vaccine encoding the malaria circumsporozoite protein from Plasmodium falciparum (PfCSP), the causative agent of malaria.…”

Section: Introductionmentioning

confidence: 99%

Safety of a GM-CSF adjuvant-plasmid DNA malaria vaccine

Monteith

Horton

et al. 2001

Gene Ther

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MuStDO 5 is a multivalent plasmid DNA vaccine for malaria comprised of five plasmid DNAs encoding five proteins from Plasmodium falciparum and one plasmid DNA encoding human GM-CSF. To evaluate the safety of MuStDO 5, a series of pre-clinical studies were conducted in mice and rabbits. In pharmacology studies in mice, GM-CSF could not be detected in the serum following either intramuscular or a combined intramuscular/intradermal administration of the vaccine, but was readily detected in the muscle following

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“…31 Briefly, three spleens from virus infected mice were removed, splenocytes stimulated in vitro for 5 days with H5.010CMVlacZ (MOI 0.5) and assayed against 51 Cr labeled target cells, which were previously infected with H5.010CMVlacZ. The CTL assay was performed using different effector:target cell ratios.…”

Section: Ctl Assaymentioning

confidence: 99%

Blunting of immune responses to adenoviral vectors in mouse liver and lung with CTLA4Ig

1998

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Vaccination with a Plasmid Vector Carrying the Rabies Virus Glycoprotein Gene Induces Protective Immunity against Rabies Virus

Cited by 364 publications

References 0 publications

New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin

New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin

Safety of a GM-CSF adjuvant-plasmid DNA malaria vaccine

Blunting of immune responses to adenoviral vectors in mouse liver and lung with CTLA4Ig

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