Vaccination with a Brucella ghost developed through a double inactivation strategy provides protection in Guinea pigs and cattle

He, Chuanyu; Yang, Jun J.; Zhao, Hailong; Liu, Mengzhi; Wu, Depei; Sun, He; Liu, Baoshan; Chen, Zeliang

doi:10.1016/j.micpath.2021.105363

Cited by 4 publications

(6 citation statements)

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“…Although extensively applied for cattle brucellosis prevention, the Brucella A19 vaccine has limitations due to its residual virulence (27). In order to solve this problem, we developed the A19 bacterial ghost vaccine and proved its safety as well as effectiveness in guinea pigs and cattle (15). To reveal the safety and effectiveness mechanism of A19BG, this study conducted proteomic and antibody profile analysis of A19BG versus A19.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, we had developed a ghost vaccine A19BG derived from B. abortus strain A19. Our results showed that A19BG provided similar protection in guinea pigs and cattle, safer than its parental strain (15). This study used comparative proteomics and protein microarray antibody profiling to gain insight into the mechanism and screen signature antigens for A19BG, which would be beneficial for brucellosis vaccine improvement and differential diagnosis.…”

Section: Introductionmentioning

confidence: 85%

“…B. abortus A19 strain and recombinant plasmid pBBR1MCS-2E containing the E-lysis gene and thermosensitive element lpR-cI857 were constructed and preserved in Tecon Biological Co., Ltd (Urumqi, China) (15).…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

“…The method for constructing A19BG was described in detail in another work (15). In brief, the fragment containing the temperature-sensitive regulation system lpR-cI857 and E-gene lysis was amplified from plasmid pBV220::E. The polymerase chain reaction products were cloned into pBBR1MCS-2 to generate the lysis plasmid pBBR1MCS-E (16,17).…”

Section: A19bg Preparation and Collectionmentioning

confidence: 99%

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Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19

Yang

et al. 2022

Front. Immunol.

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Brucellosis is an important zoonotic disease that causes great economic losses. Vaccine immunisation is the main strategy for the prevention and control of brucellosis. Although live attenuated vaccines play important roles in the prevention of this disease, they also have several limitations, such as residual virulence and difficulty in the differentiation of immunisation and infection. We developed and evaluated a new bacterial ghost vaccine of Brucella abortus A19 by a new double inactivation method. The results showed that the bacterial ghost vaccine of Brucella represents a more safe and efficient vaccine for brucellosis. We further characterised the antigenic components and signatures of the vaccine candidate A19BG. Here, we utilised a mass spectrometry-based label-free relative quantitative proteomics approach to investigate the global proteomics changes in A19BGs compared to its parental A19. The proteomic analysis identified 2014 proteins, 1116 of which were differentially expressed compared with those in A19. The common immunological proteins of OMPs (Bcsp31, Omp25, Omp10, Omp19, Omp28, and Omp2a), HSPs (DnaK, GroS, and GroL), and SodC were enriched in the proteome of A19BG. By protein micro array-based antibody profiling, significant differences were observed between A19BG and A19 immune response, and a number of signature immunogenic proteins were identified. Two of these proteins, the BMEII0032 and BMEI0892 proteins were significantly different (P < 0.01) in distinguishing between A19 and A19BG immune sera and were identified as differential diagnostic antigens for the A19BG vaccine candidate. In conclusion, using comparative proteomics and antibody profiling, protein components and signature antigens were identified for the ghost vaccine candidate A19BG, which are valuable for further developing the vaccine and its monitoring assays.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 85%

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

Section: A19bg Preparation and Collectionmentioning

confidence: 99%

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Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19

Yang

et al. 2022

Front. Immunol.

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“…Physical inactivation methods include irradiation, high-temperature heating, and ultrasound ( 12 – 14 ). Chemical inactivation methods include formalin, hydrogen peroxide, binary ethylenimine (BEI), and β-propiolactone ( 15 – 17 ). Inactivated vaccines primarily induce a systemic immune response through the production of neutralizing antibodies ( 12 ).…”

Section: Types Of Bovine Vaccinesmentioning

confidence: 99%

The combination of vaccines and adjuvants to prevent the occurrence of high incidence of infectious diseases in bovine

Yao,

Zhang,

Yang

2023

Front. Vet. Sci.

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As the global population grows, the demand for beef and dairy products is also increasing. The cattle industry is facing tremendous pressures and challenges. The expanding cattle industry has led to an increased risk of disease in cattle. These diseases not only cause economic losses but also pose threats to public health and safety. Hence, ensuring the health of cattle is crucial. Vaccination is one of the most economical and effective methods of preventing bovine infectious diseases. However, there are fewer comprehensive reviews of bovine vaccines available. In addition, the variable nature of bovine infectious diseases will result in weakened or even ineffective immune protection from existing vaccines. This shows that it is crucial to improve overall awareness of bovine vaccines. Adjuvants, which are crucial constituents of vaccines, have a significant role in enhancing vaccine response. This review aims to present the latest advances in bovine vaccines mainly including types of bovine vaccines, current status of development of commonly used vaccines, and vaccine adjuvants. In addition, this review highlights the main challenges and outstanding problems of bovine vaccines and adjuvants in the field of research and applications. This review provides a theoretical and practical basis for the eradication of global bovine infectious diseases.

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Bacterial Ghosts of the Causative Agents of Particularly Dangerous Infections

Vagaiskaya

Dentovskaya

Anisimov

2023

Problemy Osobo Opasnykh Infektsii

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Bacterial ghosts are intact walls of bacterial cells that are relieved of their contents through pores formed by mild biological or chemical methods. Methodology for generating bacterial ghosts increases the safety of killed vaccines while maintaining their antigenicity through milder preparation procedures. Moreover, bacterial ghosts can simultaneously carry several antigens or plasmid DNAs encoding protein epitopes. In recent years, there has been a growing interest in the development of prototype vaccines and systems for delivery of biologically active substances based on bacterial ghosts. This review discusses the progress in the development of this type of medications over the last years. Various methods of obtaining bacterial ghosts, their advantages and limitations are considered. The phage-mediated lysis of bacteria, molecular manipulations with lysis genes, difficulties encountered in scaling the biotechnological production of bacterial ghosts, and ways to overcome them are described in detail. The use of bacterial ghosts as alternative killed vaccines, adjuvants, recombinant antigenic platform, carrier of plasmid DNA by the models of pathogens of particularly dangerous infections of bacterial etiology is investigated.

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Vaccination with a Brucella ghost developed through a double inactivation strategy provides protection in Guinea pigs and cattle

Cited by 4 publications

References 35 publications

Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19

Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19

The combination of vaccines and adjuvants to prevent the occurrence of high incidence of infectious diseases in bovine

Bacterial Ghosts of the Causative Agents of Particularly Dangerous Infections

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