Vaccination using inactivated Mycoplasma pneumoniae induces detrimental infiltration of neutrophils after subsequent infection in mice

Tamiya, Shigeyuki; Yoshikawa, Eisuke; Ogura, Monami; Kuroda, Etsushi; Suzuki, Koichiro; Yoshioka, Yasuo

doi:10.1016/j.vaccine.2020.05.074

Cited by 10 publications

(10 citation statements)

References 39 publications

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“…In our reinfection experiment, we found that prior Mp infection resulted in increased infiltration of CD45 + immune cells and neutrophils into BALF after subsequent reinfection in DBA/2 mice but not in BALB/c mice (Figure 5). In a previous report, we revealed that CD4 + T cells do not induce neutrophil infiltration after Mp infection in naive mice (Tamiya et al, 2020). Collectively, these data suggest that Mp-specific Th1 and/or Th17 cells induced by prior Mp infection were the major effector cells for the enhanced neutrophil infiltration observed in DBA/2 mice after Mp reinfection.…”

Section: Discussionsupporting

confidence: 52%

“…Mp (strain: FH) was purchased from the American Type Culture Collection (Manassas, VA, USA). Mp was cultured as described previously (Tamiya et al, 2020). Mp in PPLO medium at 2 × 10 9 colony-forming units (CFU)/ml was used.…”

Section: Culture Condition Of Mycoplasma Pneumoniaementioning

confidence: 99%

“…The Mp load in BALF was analyzed by using real-time polymerase chain reaction (real-time PCR) as described previously (Tamiya et al, 2020). To measure the Mp bacterial load by Mp culture, BALF was serially diluted and cultured on a PPLO agar plate, which contained PPLO agar (Difco Laboratories), 10% horse serum (Thermo Fisher Scientific), 0.25% yeast extract (Difco Laboratories), 1000 U/ml penicillin G, and 0.25% glucose.…”

Section: Quantification Of Mycoplasma Pneumoniae By Bacterial Culturementioning

confidence: 99%

“…The number of immune cells in the BALF was determined as previously described (Tamiya et al, 2020). Briefly, the cell suspension in BALF was analyzed by flow cytometry (NovoCyte Flow Cytometer, ACEA Bioscience, San Diego, CA, USA).…”

Section: Immune Cell Analysis Of Bronchoalveolar Lavage Fluidmentioning

confidence: 99%

“…On day 14, plasma and spleen samples were collected and analyzed as follows. The plasma levels of Mp-and P1-specific IgG were determined using ELISA as described previously (Tamiya et al, 2020). To detect P1specific total IgG, ELISA plates (Corning, Corning, NY, USA) were coated overnight at 4°C with recombinant P1 (1 mg/ml) in PBS.…”

Section: Immune Responses To Mycoplasma Pneumoniaementioning

confidence: 99%

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Susceptibility Analysis in Several Mouse Strains Reveals Robust T-Cell Responses After Mycoplasma pneumoniae Infection in DBA/2 Mice

Tamiya

Yoshikawa

Suzuki

et al. 2021

Front. Cell. Infect. Microbiol.

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Mycoplasma pneumoniae (Mp) is a highly contagious respiratory pathogen responsible for human community-acquired pneumonia. The number of antibiotic-resistant Mp strains is increasing; therefore, to develop novel therapeutics, it is crucial to precisely understand the pathogenesis of mycoplasma pneumonia. Herein, we examined the susceptibility and response to Mp among eight inbred mouse strains. Following infection, the bacterial load in the bronchoalveolar lavage fluid (BALF) from DBA/2 mice was higher than that in the other tested strains such as BALB/c mice, which are frequently used in Mp research. In contrast, the numbers of CD45+ immune cells and neutrophils in BALF were comparable between BALB/c and DBA/2 mice, with lower numbers observed in C57BL/6J and CBA/N mice than in BALB/c mice. Among the tested strains, the BALF level of interleukin 12 subunit p40 was highest in DBA/2 mice; however, significant differences in other cytokines levels were not observed between BALB/c and DBA/2 mice. After Mp infection, Mp-specific Th1 and Th17 responses were significantly enhanced in DBA/2 mice when compared with BALB/c mice. Furthermore, prior infection with Mp increased the number of neutrophils in BALF after the reinfection of DBA/2 mice through an Mp-specific CD4+ T cell-dependent mechanism. Thus, DBA/2 may be an appropriate strain for evaluating Mp infection. Moreover, a comparison of responses revealed by various inbred mouse strains could be useful for elucidating the pathogenesis of Mycoplasma pneumonia.

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Section: Discussionsupporting

confidence: 52%

Section: Culture Condition Of Mycoplasma Pneumoniaementioning

confidence: 99%

Section: Quantification Of Mycoplasma Pneumoniae By Bacterial Culturementioning

confidence: 99%

Section: Immune Cell Analysis Of Bronchoalveolar Lavage Fluidmentioning

confidence: 99%

Section: Immune Responses To Mycoplasma Pneumoniaementioning

confidence: 99%

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