Vaccination strategy is an important determinant in immunological outcome and survival in Arctic charr (Salvelinus alpinus) when challenged with atypical Aeromonas salmonicida

Brown, Alyson; Whyte, S. K.; Braden, Laura M.; Groman, David B.; Purcell, Sara L.; Fast, Mark D.

doi:10.1016/j.aquaculture.2019.734838

Cited by 3 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melt curves showed single product formation and absence of primer dimers for all transcripts tested. The qPCR protocol was as described previously ( 32 ). Specifically, the qPCR was conducted on CFX96 Touch Real-Time PCR System (Bio-Rad), using the following thermal program: initial activation of 95°C for 30 s, 95°C for 15 s then 60°C for 30 s (40 cycles), followed by a melt curve analysis from 65 to 95°C with fluorescence being read every 0.5 s with a ramp rate of 0.5°C.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of Atlantic Salmon (Salmo salar) Skin in Response to Sea Lice and Infectious Salmon Anemia Virus Co-Infection Under Different Experimental Functional Diets

et al. 2022

Self Cite

View full text Add to dashboard Cite

Sea lice (Lepeophtheirus salmonis) are ectoparasitic copepods that cause significant economic loss in marine salmoniculture. In commercial salmon farms, infestation with sea lice can enhance susceptibility to other significant pathogens, such as the highly contagious infectious salmon anemia virus (ISAv). In this study, transcriptomic analysis was used to evaluate the impact of four experimental functional feeds (i.e. 0.3% EPA/DHA+high-ω6, 0.3% EPA/DHA+high-ω6+immunostimulant (IS), 1% EPA/DHA+high-ω6, and 1% EPA/DHA+high-ω3) on Atlantic salmon (Salmo salar) during a single infection with sea lice (L. salmonis) and a co-infection with sea lice and ISAv. The overall objectives were to compare the transcriptomic profiles of skin between lice infection alone with co-infection groups and assess differences in gene expression response among animals with different experimental diets. Atlantic salmon smolts were challenged with L. salmonis following a 28-day feeding trial. Fish were then challenged with ISAv at 18 days post-sea lice infection (dpi), and maintained on individual diets, to establish a co-infection model. Skin tissues sampled at 33 dpi were subjected to RNA-seq analysis. The co-infection’s overall survival rates were between 37%-50%, while no mortality was observed in the single infection with lice. With regard to the infection status, 756 and 1303 consensus differentially expressed genes (DEGs) among the four diets were identified in “lice infection vs. pre-infection” and “co-infection vs. pre-infection” groups, respectively, that were shared between the four experimental diets. The co-infection groups (co-infection vs. pre-infection) included up-regulated genes associated with glycolysis, the interferon pathway, complement cascade activity, and heat shock protein family, while the down-regulated genes were related to antigen presentation and processing, T-cell activation, collagen formation, and extracellular matrix. Pathway enrichment analysis conducted between infected groups (lice infection vs. co-infection) resulted in several immune-related significant GO terms and pathways unique to this group, such as “autophagosome”, “cytosolic DNA-sensing pathway” and “response to type I interferons”. Understanding how experimental functional feeds can impact the host response and the trajectory of co-infections will be an essential step in identifying efficacious intervention strategies that account for the complexities of disease in open cage culture.

show abstract

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of Atlantic Salmon (Salmo salar) Skin in Response to Sea Lice and Infectious Salmon Anemia Virus Co-Infection Under Different Experimental Functional Diets

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are some relationships between gene expression profiles and the immune response to vaccination or challenge. Up‐regulation of complement and acute phase genes as well as cellular signalling was found in Atlantic salmon ( Salmo salar ) vaccinated with two commercial vaccines against A. salmonicia (Brown et al., 2020). Labarca et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of propolis on the immune enhancement of the formalin‐inactivated Aeromonas salmonicida vaccine

et al. 2020

View full text Add to dashboard Cite

Furunculosis caused by Aeromonas salmonicida is a major threat to salmonids throughout all their life stages. Vaccination against A. salmonicida has been shown to provide effective protection in aquaculture. The success of many vaccines relies on their association with the appropriate adjuvant to increase their immunogenicity and ensure long-term protection. In this study, the effects of vaccines using propolis as adjuvants against furunculosis in rainbow trout (Oncorhynchus mykiss) were evaluated. The parameters evaluated included the relative percent survival (RPS), the specific IgM antibody level and the expression profiles of several immune-related genes in four tissues following immunization and challenge. The results showed that the experimental vaccines containing propolis as adjuvants (ExpVacPro group) could effectively protect the experimental fish against A. salmonicida infection with an RPS of 89.47%. The gene expression data revealed that ExpVacPro could induce the differential expression of the pro-inflammatory cytokines, MHC class Ⅰ, T-cell markers and Ig markers in immunized fish, with higher levels in liver and spleen than in head kidney and gill. ExpVacPro initially increased the expression of MHC I and IL8 genes in related pathways, and then gradually increased the expression of IL-1β and IgT genes for about 2 weeks. During the post-vaccination challenge, the expression levels of all detected genes were up-regulated especially for CD8 and IgT genes. The specific IgM antibody titres were significantly induced following immunization and challenge. In conclusion, the results indicated that propolis could improve potency and efficacy of formalin-inactivated A. salmonicida vaccine as adjuvants.

show abstract

Aeromonas salmonicida infection kinetics and protective immune response to vaccination in sablefish (Anoplopoma fimbria)

Vásquez

Cao

Hossain

et al. 2020

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Vaccination strategy is an important determinant in immunological outcome and survival in Arctic charr (Salvelinus alpinus) when challenged with atypical Aeromonas salmonicida

Cited by 3 publications

References 55 publications

Transcriptome Analysis of Atlantic Salmon (Salmo salar) Skin in Response to Sea Lice and Infectious Salmon Anemia Virus Co-Infection Under Different Experimental Functional Diets

Transcriptome Analysis of Atlantic Salmon (Salmo salar) Skin in Response to Sea Lice and Infectious Salmon Anemia Virus Co-Infection Under Different Experimental Functional Diets

Effects of propolis on the immune enhancement of the formalin‐inactivated Aeromonas salmonicida vaccine

Aeromonas salmonicida infection kinetics and protective immune response to vaccination in sablefish (Anoplopoma fimbria)

Contact Info

Product

Resources

About