Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption

Burrells, Alison; Benavides, Julio; Cantón, Germán; Garcia, João Luís; Bartley, Paul M.; Nath, Mintu; Thomson, Jackie; Chianini, Francesca; Innes, Elisabeth A.; Katzer, Frank

doi:10.1186/s13567-015-0177-0

Cited by 44 publications

(35 citation statements)

References 41 publications

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“…All pigs in group 1, 2 and 3 were seropositive by ELISA by day 21 (for further details refer to (Burrells et al, 2015)). Mouse bioassay and MC-PCR results are presented in Table 13.…”

Section: Resultsmentioning

confidence: 99%

“…(Full details of the results of the vaccination trial, including all tissues tested by mouse bioassay and ITS1 PCR can be obtained from the published manuscript (Burrells et al, 2015)). …”

Section: Discussionmentioning

confidence: 99%

“…In that case, vaccination of food animals other than sheep can be valuable for the prevention of human toxoplasmosis. Therefore, experimental infections by oral inoculation with tissue cysts or oocysts in unvaccinated and Toxovax-vaccinated pigs have been carried out at Moredun Research Institute (Burrells et al, 2015). Mouse bioassay and ITS1 nested PCR had already been completed for this study in brain, a pooled sample of heart, tongue, masseter and diaphragm, and a pooled sample of chop, loin, left hind limb, and left forelimb and results are available for comparison, within the present EFSA project, between bioassay and MC-PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Vaccination and challenge experiments had previously been peformed and published (Katzer et al, 2014;Burrells et al, 2015). In the present project, tissues were additionally tested by MC-PCR to analyse the effect of vaccination on parasite load.…”

Section: Relationship Between Direct and Indirect Detection Methods Imentioning

confidence: 99%

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Experimental studies on Toxoplasma gondii in the main livestock species (GP/EFSA/BIOHAZ/2013/01) Final report

Opsteegh

Schares

Blaga

et al. 2016

EFS3

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After extensive review of the literature, experimental studies were performed in the main livestock species (cattle, small ruminants, pigs, horses and poultry) to address the main knowledge gaps considering (1) the relationship between detection of antibodies and presence of T. gondii, (2) the anatomical distribution of tissue cysts, and (3) on-farm risk factors for infection. In slaughterhouse studies in cattle and horses, a lack of concordance between detection of antibodies by MAT and detection of T. gondii in tissues by mouse bioassay or MC-PCR was demonstrated. Positive mouse bioassay results were obtained in cattle and horses, indicating a potential infection risk for consumers. DISCLAIMERThe present document has been produced and adopted by the bodies identified above as author(s). In accordance with Article 36 of Regulation (EC) No 178/2002, this task has been carried out exclusively by the author(s) in the context of a grant agreement between the European Food Safety Authority and the author(s). The present document is published complying with the transparency principle to which the Authority is subject. It cannot be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. The present document has been produced and adopted by the bodies identified above as author(s). In accordance with Article 36 of Regulation (EC) No 178/2002, this task has been carried out exclusively by the author(s) in the context of a grant agreement between the European Food Safety Authority and the author(s). The present document is published complying with the transparency principle to which the Authority is subject. It cannot be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. 2In naturally infected pigs and chickens the agreement depended on assays and matrices/analytes used, but was considered fair to substantial. Calves, sheep, pigs, chickens and turkeys were experimentally infected with T. gondii. In calves various tissues were tested and T. gondii was recovered, but no clear predilection sites were identified. There was also little variation in parasite load between the different edible tissues (i.e. skeletal muscles) from pigs and sheep. Brain and heart were clearly identified as predilection sites in chickens and turkeys, with higher parasite loads compared to breast, thigh and drumstick muscle. The observation that drumstick, an edible tissue, tested positive in 25% (8/32) of the bioassay positive organic laying hens, indicated a potential risk for consumers. The seroprevalence of T. gondii in Dutch indoor-housed dairy goats appears to be relatively low and was associated with the presence of cats at the farm. I...

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“…All pigs in group 1, 2 and 3 were seropositive by ELISA by day 21 (for further details refer to (Burrells et al, 2015)). Mouse bioassay and MC-PCR results are presented in Table 13.…”

Section: Resultsmentioning

confidence: 99%

“…(Full details of the results of the vaccination trial, including all tissues tested by mouse bioassay and ITS1 PCR can be obtained from the published manuscript (Burrells et al, 2015)). …”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Relationship Between Direct and Indirect Detection Methods Imentioning

confidence: 99%

See 2 more Smart Citations

Experimental studies on Toxoplasma gondii in the main livestock species (GP/EFSA/BIOHAZ/2013/01) Final report

Opsteegh

Schares

Blaga

et al. 2016

EFS3

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show abstract

“…A vaccine is available for sheep and goats that can prevent miscarriage owing to toxoplasmosis, says Elisabeth Innes, an immunologist at Moredun Research Institute near Edinburgh, UK, and one of the vaccine's developers. Her team has shown that the vaccine can also reduce the number of tissue cysts in food animals, potentially making the food supply safer 6 .…”

Section: Vaccine Vexationmentioning

confidence: 99%

Parasites: Kitty carriers

DeWeerdt¹

2017

Nature

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Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia

et al. 2017

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Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.

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Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption

Cited by 44 publications

References 41 publications

Experimental studies on Toxoplasma gondii in the main livestock species (GP/EFSA/BIOHAZ/2013/01) Final report

Experimental studies on Toxoplasma gondii in the main livestock species (GP/EFSA/BIOHAZ/2013/01) Final report

Parasites: Kitty carriers

Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia

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