Vaccination of lambs with the recombinant protein rHc23 elicits significant protection against Haemonchus contortus challenge

Fawzi, Elshaima M.; González‐Sánchez, Elena; Corral, María Jesús; Alunda, José María; Cuquerella, Montserrat

doi:10.1016/j.vetpar.2015.04.029

Cited by 24 publications

(28 citation statements)

References 24 publications

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“…This protein was then used to vaccinate five-to six-month-old lambs three times in the context of aluminium hydroxide adjuvant. 87 The lambs were subsequently challenged with 15 000 H, contortus third-stage larvae and were found to be significantly protected against challenge, with more than an 80% reduction in FECs and average abomasal parasite burdens (compared to challenge controls) 45 days after challenge. This latest work with the Hc23 antigen indicates that it is now lead subunit vaccine candidate for this nematode.…”

Section: A Recombinant Vaccine For Haemonchosismentioning

confidence: 97%

“…Subsequently, the gene encoding the Hc23 antigen was identified, and a recombinant version expressed in E. coli . This protein was then used to vaccinate five‐ to six‐month‐old lambs three times in the context of aluminium hydroxide adjuvant . The lambs were subsequently challenged with 15 000 H, contortus third‐stage larvae and were found to be significantly protected against challenge, with more than an 80% reduction in FECs and average abomasal parasite burdens (compared to challenge controls) 45 days after challenge.…”

Section: Current Status Of Subunit Vaccines For the Main Gin Targets mentioning

confidence: 99%

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Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Matthews

Geldhof

Tzelos

et al. 2016

Parasite Immunology

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SummaryThe global increase in anthelmintic resistant nematodes of ruminants, together with consumer concerns about chemicals in food, necessitates the development of alternative methods of control for these pathogens. Subunit recombinant vaccines are ideally placed to fill this gap. Indeed, they are probably the only valid option for the long-term control of ruminant parasitic nematodes given the increasing ubiquity of multidrug resistance in a range of worm species across the world. The development of a subunit multicellular parasite vaccine to the point of practical application would be a groundbreaking step in the control of these important endemic infections of livestock. This review summarizes the current status of subunit vaccine development for a number of important gastrointestinal nematodes of cattle and sheep, with a focus on the limitations and problems encountered thus far, and suggestions as to how these hurdles might be overcome. K E Y W O R D Sgastrointestinal nematodes, ruminants, vaccines

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Section: A Recombinant Vaccine For Haemonchosismentioning

confidence: 97%

Section: Current Status Of Subunit Vaccines For the Main Gin Targets mentioning

confidence: 99%

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Matthews

Geldhof

Tzelos

et al. 2016

Parasite Immunology

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“…Until now, many native and recombinant proteins have been investigated in different immunization trials against H. contortus and most cases demonstrated variable reduction in fecal egg output and worm burdens [3,[6][7][8][9]. DNA vaccines have also been tested and showed partial protection against H. contortus [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Immunization of Goats with Recombinant Protein 14-3-3 Isoform 2(rHcftt-2) Induced Moderate Protection against Haemonchus contortus Challenge

Jia

Tian

et al. 2020

Pathogens

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A previous study identified that isoform 2 (Hcftt-2) of the 14-3-3 protein of Haemonchus contortus (H. contortus) could suppress immune functions of goat peripheral blood mononuclear cells (PBMCs) and might be a potential vaccine target, as neutralization of the protein function may enhance anti-parasite immunity. In this research, the recombinant Hcftt-2 was evaluated for its immunoprotective efficacy against H. contortus infection in goats. Five experimental goats were immunized twice with rHcftt-2 along with Freund’s adjuvant. The five immunized goats and five nonimmunized goats (adjuvant only) were challenged with 5000 L3-stage H. contortus larvae after 14 days of second immunization. Five nonimmunized and uninfected goats (adjuvant only) were set as the uninfected group. A significant increase in the serum immunoglobin G(IgG) and serum IgA levels were identified in the rHcftt-2 immunized animals. The mean eggs per gram in feces (EPG) and the worm burdens of rHcftt-2 immunized group were reduced by 26.46% (p < 0.05) and 32.33%, respectively. In brief, immunization of goats with rHcftt-2 induced moderate protection against H. contortus challenge.

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“…Lambs vaccinated with the recombinant Hc23 antigen were significantly protected against an artificial challenge infection, with more than 80% reduction in fecal egg counts (FECs) and average abomasal parasite burdens, compared with challenge controls. 13 This work is a promising step toward a recombinant Haemonchus vaccine, but further experiments are needed to confirm these results and to test the vaccine in natural infection conditions.…”

Section: Haemonchus Contortusmentioning

confidence: 84%

Helminth Vaccines in Ruminants

Claerebout

Geldhof

2020

Veterinary Clinics of North America: Food Animal Practice

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KEYWORDS Gastrointestinal nematodes Lungworm Liver fluke Vaccines KEY POINTSOnly a few vaccines against parasitic helminths of livestock are currently on the market; namely those against Dictyocaulus viviparus in cattle, Haemonchus contortus in sheep, and the tapeworm Echinococcus granulosus in sheep.Research is ongoing to develop vaccines based on purified or recombinantly expressed worm proteins for Ostertagia ostertagi and Cooperia oncophora in cattle, for Teladorsagia circumcincta and H contortus in sheep, and for Fasciola hepatica in ruminants. Recombinant expression of worm antigens with the correct conformation to induce protective immunity is still a major challenge in vaccine development. Vaccination is likely to be part of integrated worm control, together with other control measures, such as anthelmintic treatments, biological control, and grazing management. Interdisciplinary research, including social sciences, should investigate drivers of farmers' and veterinarians' decision making, to optimize uptake of vaccination by the end users.Vet Clin Food Anim 36 (2020) 159-171

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Vaccination of lambs with the recombinant protein rHc23 elicits significant protection against Haemonchus contortus challenge

Cited by 24 publications

References 24 publications

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Immunization of Goats with Recombinant Protein 14-3-3 Isoform 2(rHcftt-2) Induced Moderate Protection against Haemonchus contortus Challenge

Helminth Vaccines in Ruminants

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