Vaccination of channel catfish, Ictalurus punctatus (Rafinesque), by immersion and oral booster against Edwardsiella ictaluri

Abstract

A commercially prepared vaccine against Edwardsiella ictaluri was used to vaccinate 12-day-old channel catfish fry by immersion, or by immersion plus an oral booster 2 months later. One month after the fish were fed the booster vaecine, they were challenged by waterborne exposure to 21 x 10" eells ml~' of E. ictaluri. Immersion only vaccinated fish suffered 6-7% mortality and immersion plus oral-boosted fish had a 3-3% mortality. Mortality among non-vaccinated controls was 96-7% and was significantly (/*<0-01) above the vaccinated mortality. The relative per cent survival for the immersion-only fish was 93-1, while it was 96-6 for the immersion plus orai-boosted fish. Agglutinating antibody titres of the vaccinated fish were signifieantly {F<005) higher than the control fish. When the ponds were drained 6 months after stocking, 42 7% of non-vaecinated, 56-3% of immersion-only and 70-8% of immersion plus oral-boosted fish were harvested. Survival of immersion plus orally-boosted fish was significantly (P<0-05) higher than the controls of immersion-only fish. Duplieate populations of immersion plus oral-booster-vaccinated fish grew 34% and 56% faster, respectively, on an average daily gain than the control fish, while immersiononly fish in one pond grew 20% less per day and fish in the second pond grew 48% faster.

Cholera toxin has adjuvant properties in channel catfish when injected intraperitoneally

“…Increased survival following challenge of channel catfish with E. ictaluri has been previously observed after bath vaccination followed by an oral booster (Plumb & Vinitnantharat, 1993;Thune et al, 1993). Plumb & Vinitnantharat (1993) found that in fish the agglutination titres detected after immersion (killed bacterin at 12 days) plus oral vaccination (presented in the feed 3 months later) was higher than in fish …”

“…Plumb & Vinitnantharat (1993) found that in fish the agglutination titres detected after immersion (killed bacterin at 12 days) plus oral vaccination (presented in the feed 3 months later) was higher than in fish …”

“…Oral vaccination of fish has been studied in ayu (Plecoglossus altivelis) (Kawai et al, 1981), (Pleuronectes platessa L.) (Fletcher et al, 1973), tilapia (Oreochromis mossambicus) (Jenkins et al, 1994a(Jenkins et al, , 1994b, rainbow trout (Oncorhynchus mykiss) (Georgopoulou et al, 1986) and channel catfish (Ictalurus punctatus R.) (Plumb & Vinitnantharat, 1993;Plumb et al, 1994aPlumb et al, , 1994bThune et al, 1993;Ainsworth et al, 1995). Thune et al (1993) and Plumb & Vinitnantharat (1993) have shown evidence of the protective properties of oral vaccinations with E. ictaluri in channel catfish.…”

“…Thune et al (1993) and Plumb & Vinitnantharat (1993) have shown evidence of the protective properties of oral vaccinations with E. ictaluri in channel catfish. These studies, however, have come prior to definitive studies of mucosal immunity.…”

Vaccine efficacy of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Edwardsiella ictaluri against E. tarda in tilapia

“…Vaccine preparation: Preparation of the formalin-killed WC vaccine was prepared as previously described by Plumb and Vinitnantharat (1993) and Liu et al (2005) with modifications as follows. The E. ictaluri strain OT9606S was grown in BHIB with shaking at 150 rpm for 36 48 h at 25 C, to obtain a final concentration of 1 10 9 cells ml 1 .…”