Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen

Subharat, Supatsak; Shu, Dairu; Zheng, Tao; Buddle, Bryce M.; Janssen, Peter H.; Luo, Dongwen; Wedlock, D. Neil

doi:10.1016/j.vetimm.2015.02.008

Cited by 26 publications

(20 citation statements)

References 25 publications

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“…IgA in plasma provides the source for secretory IgA via gut epithelia or through saliva, which in ruminants represent the main vehicle of introducing immunoglobulins into the rumen [ 2 ]. Subharat et al [ 64 ] recently showed that IgA resist longer in the rumen to degradation than IgG, due to the secretory component of IgA which makes the immunoglobulin more resistant to proteases [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Natural and artificial feeding management before weaning promote different rumen microbial colonization but not differences in gene expression levels at the rumen epithelium of newborn goats

et al. 2017

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The aim of this work was to evaluate the effect of feeding management during the first month of life (natural with the mother, NAT, or artificial with milk replacer, ART) on the rumen microbial colonization and the host innate immune response. Thirty pregnant goats carrying two fetuses were used. At birth one kid was taken immediately away from the doe and fed milk replacer (ART) while the other remained with the mother (NAT). Kids from groups received colostrum during first 2 days of life. Groups of four kids (from ART and NAT experimental groups) were slaughtered at 1, 3, 7, 14, 21 and 28 days of life. On the sampling day, after slaughtering, the rumen content was sampled and epithelial rumen tissue was collected. Pyrosequencing analyses of the bacterial community structure on samples collected at 3, 7, 14 and 28 days showed that both systems promoted significantly different colonization patterns (P = 0.001). Diversity indices increased with age and were higher in NAT feeding system. Lower mRNA abundance was detected in TLR2, TLR8 and TLR10 in days 3 and 5 compared to the other days (7, 14, 21 and 28). Only TLR5 showed a significantly different level of expression according to the feeding system, presenting higher mRNA abundances in ART kids. PGLYRP1 showed significantly higher abundance levels in days 3, 5 and 7, and then experienced a decline independently of the feeding system. These observations confirmed a highly diverse microbial colonisation from the first day of life in the undeveloped rumen, and show that the colonization pattern substantially differs between pre-ruminants reared under natural or artificial milk feeding systems. However, the rumen epithelial immune development does not differentially respond to distinct microbial colonization patterns.

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Section: Discussionmentioning

confidence: 99%

Natural and artificial feeding management before weaning promote different rumen microbial colonization but not differences in gene expression levels at the rumen epithelium of newborn goats

et al. 2017

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“…Developing vaccines against methanogens in the rumen of ruminant animals offers an attractive mitigation approach to reduce enteric CH 4 emissions from ruminant animals, due to its low cost, prolonged efficacy and little side effect [ 28 ]. The bioinformation techniques can provide information to identify potential vaccine targets in theory [ 17 ] and one of which, GT2, as a vaccination given to cattle, could produce stable salvia antibody in rumen [ 29 ]. The EhaF, which is another potential vaccine target, plays an essential anaplerotic role in hydrogenotrophic methanogenesis and is essential for growth of methanogens[ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Immunization against Rumen Methanogenesis by Vaccination with a New Recombinant Protein

et al. 2015

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Vaccination through recombinant proteins against rumen methanogenesis provides a mitigation approach to reduce enteric methane (CH4) emissions in ruminants. The objective of present study was to evaluate the in vivo efficacy of a new vaccine candidate protein (EhaF) on methanogenesis and microbial population in the rumen of goats. We amplified the gene mru 1407 encoding protein EhaF using fresh rumen fluid samples of mature goats and successfully expressed recombinant protein (EhaF) in Escherichia coli Rosetta. This product was evaluated using 12 mature goats with half for control and other half injected with 400ug/goat the purified recombinant protein in day 1 and two subsequent booster immunizations in day 35 and 49. All measurements were undertaken from 63 to 68 days after the initial vaccination, with CH4 emissions determined using respiration calorimeter chambers. The results showed that the vaccination caused intensive immune responses in serum and saliva, although it had no significant effect on total enteric CH4 emissions and methanogen population in the rumen, when compared with the control goats. However, the vaccination altered the composition of rumen bacteria, especially the abundance of main phylum Firmicutes and genus Prevotella. The results indicate that protein EhaF might not be an effective vaccine to reduce enteric CH4 emissions but our vaccine have potential to influence the rumen ecosystem of goats.

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“…This will require the identification of some common features, such as a shared surface protein, unique to Archaea, so as not to interfere with or compromise the function of other bacterial consortia playing crucial roles in ruminal digestion. Recent trials using a recombinant protein as a potential antigen against methanogens elicited strong antibody responses in both cattle (Subharat et al 2015) and sheep (Subharat et al 2016), but neither study quantified rumen methanogens or enteric methane production post-immunisation. Using a different protein, but employing a similar approach, Zhang et al (2015) also observed strong immune responses in saliva and plasma, yet failed to detect any reduction in either rumen methanogens or enteric methane production in inoculated goats.…”

Section: Host Response: Immunisationmentioning

confidence: 99%

Creating a low enteric methane emission ruminant: what is the evidence of success to the present and prospects for developing economies?

Goopy¹

2019

Anim. Prod. Sci.

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Enteric methane emissions from livestock constitute a greater part of anthropogenic greenhouse gases (GHGs) in Africa, than in more industrialised economies, providing a strong incentive for the development of low methane phenotype ruminants. Although dietary and husbandry options already exist for lowering methane production, means of changing 'methane status' of animals enduringly has a strong appeal. This paper is a critical review the empirical success to date of attempts to alter this status. Introduction of reductive acetogens, defaunation, anti-methanogen vaccines, early life programming and genetic selection at both the rumen and animal level are considered in turn. It is concluded that to date, there is little in vivo evidence to support the practical success of any of these strategies, save selective breeding, and this at a high cost with unknown efficacy. Finally, it is suggested that for developing economies management and nutritional strategies to reduce emissions will have the greatest and most immediate impact, at the lowest cost.

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Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen

Cited by 26 publications

References 25 publications

Natural and artificial feeding management before weaning promote different rumen microbial colonization but not differences in gene expression levels at the rumen epithelium of newborn goats

Natural and artificial feeding management before weaning promote different rumen microbial colonization but not differences in gene expression levels at the rumen epithelium of newborn goats

Immunization against Rumen Methanogenesis by Vaccination with a New Recombinant Protein

Creating a low enteric methane emission ruminant: what is the evidence of success to the present and prospects for developing economies?

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