Vaccination-induced IgG response to Galα1–3GalNAc glycan epitopes in lambs protected against Haemonchus contortus challenge infection

Stijn, Caroline M.W. van; Broek, Marloes van den; Vervelde, Lonneke; Alvarez, Richard A.; Cummings, Richard D.; Tefsen, Boris; Die, Irma van

doi:10.1016/j.ijpara.2009.07.009

Cited by 37 publications

(33 citation statements)

References 33 publications

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“…The enzymatic origin of the second GalFuc modification is certainly less clear; it can be assumed that one of the five ␣1,3-fucosyltransferase homologues in C. elegans (26) is responsible for transfer of fucose to the distal core GlcNAc. On the other hand, there are no clear ␣-galactosyltransferase homologues in C. elegans; perhaps the only ␣-galactosyltransferase activity known to date in nematode extracts, specifically in H. contortus, uses GalNAc and not fucose as an acceptor (42). Perhaps coincidentally, the N-glycans modified by fucose on the distal core GlcNAc lack ␣1,6-mannose residues; thus, a mannosidase activity is either a pre-requisite for modification of the distal GlcNAc or this modification is a "GO" signal for a mannosidase.…”

Section: Biosynthesis and Structure Of N-glycan Core Modifications Inmentioning

confidence: 99%

Galactosylated Fucose Epitopes in Nematodes

Yan

Bleuler-Martinez

Plaza

et al. 2012

Journal of Biological Chemistry

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Background: Galactosylation of core fucose residues is a peculiar feature of invertebrate N-glycans. Results: Two variants of galactosylated fucose were observed in a Caenorhabditis elegans mutant and in two nematode parasites. Conclusion: Galactosylation of fucose in nematodes is more widespread than previously thought and is affected by processing hexosaminidases. Significance: Galactosylated fucose motifs may present new targets for anthelminthic agents.

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Section: Biosynthesis and Structure Of N-glycan Core Modifications Inmentioning

confidence: 99%

Galactosylated Fucose Epitopes in Nematodes

Yan

Bleuler-Martinez

Plaza

et al. 2012

Journal of Biological Chemistry

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“…The latter are easier to produce and distribute, and many of them are proteases, cysteine proteases, metalloproteases, aspartyl proteases (PEPs), all proteins from the helminth intestine, and some with unknown biological functions (8)(9)(10)(11). Due to the homology in their sequences, some of these antigens have proved to be effective against different nematode species (3,12,13). In addition, most of the adjuvants used are oily suspensions such as Freund's adjuvant, Montanide, or Lipovant (14-16).…”

mentioning

confidence: 99%

Intranasal Immunization of Lambs with Serine/Threonine Phosphatase 2A against Gastrointestinal Nematodes

Fawzi

Cruz‐Bustos

Samblas

et al. 2013

Clin Vaccine Immunol

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b Seven 3-month-old, female, helminth-free lambs were immunized intranasally with three doses (1 mg total) of a recombinant part of the catalytic region of the serine/threonine phosphatase 2A (PP2Ar) (group 1 [G1]). In addition, four lambs were used as an adjuvant control group (G2), four as unimmunized, infected controls (G3), and four as unimmunized, uninfected controls (G4). Fifteen days after the last immunization, lambs from G1, G2, and G3 were challenged with 10,000 larval stage 3 (L3) organisms in a plurispecific nematode infection composed of ca. 40% Trichostrongylus colubriformis, 40% Haemonchus contortus, and 20% Teladorsagia circumcincta. All the lambs were clinically monitored throughout the experiment. Parasitological (fecal egg output and immunological response), biopathological (packed-cell volume and leukocyte and eosinophil counts), and zootechnical (live-weight gain) analyses were conducted. On day 105 of the experiment, all the animals were slaughtered and the adult worm population in their abomasa examined. Intranasal administration of PP2Ar with bacterial walls as an adjuvant elicited a strong immune response in the immunized lambs, as evidenced by their humoral immune response. Immunized animals and animals receiving the adjuvant shed significantly (P < 0.001) fewer numbers of parasites' eggs in their feces. The immunization significantly reduced the helminth burden in the abomasa by the end of the experiment (>68%), protection being provided against both Haemonchus and Teladorsagia. Live-weight gain in the immunized lambs was similar to that in the uninfected controls versus the infected or adjuvanted animal groups. Our results suggest that heterologous immunization of ruminants by intranasal administration may be efficacious in the struggle to control gastrointestinal helminths in these livestock.

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“…Vaccination of lambs with alhydrogel-adjuvant excreted/secreted products of the nematode H. contortus conferred a high level of protection which was correlated with IgG antibodies to LDNF and Galα1-3GalNAc (257, 272). Other studies that used natively purified activation-associated secreted proteins (ASPs) from the cattle nematode O. ostertagi also afforded protection, and showed that the recombinantly produced ASPs from E. coli were unable to induce protection or any antibodies to native ASPs (273).…”

Section: Adaptive Immune Responses To Helminth-derived Glycansmentioning

confidence: 97%

“…Studies have shown that LDNF, which is more easily produced in the lab than tyvelose, is also a sensitive indicator of T. spiralis infection (256). Similarly, results from glycan microarray analyses have shown that the sheep nematode H. contortus possesses Galα1-3GalNAc, which is antigenic and uncommon among nematodes and trematodes (257). These new microarray technologies have the promising ability to screen a single sample for antibodies to multiple glycans from different helminth species, many of which are co-endemic, in a microscale assay.…”

Section: Adaptive Immune Responses To Helminth-derived Glycansmentioning

confidence: 99%

Glycoconjugates in Host-Helminth Interactions

Prasanphanich¹,

Mickum²,

Heimburg-Molinaro³

et al. 2013

Front. Immunol.

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Helminths are multicellular parasitic worms that comprise a major class of human pathogens and cause an immense amount of suffering worldwide. Helminths possess an abundance of complex and unique glycoconjugates that interact with both the innate and adaptive arms of immunity in definitive and intermediate hosts. These glycoconjugates represent a major untapped reservoir of immunomodulatory compounds, which have the potential to treat autoimmune and inflammatory disorders, and antigenic glycans, which could be exploited as vaccines and diagnostics. This review will survey current knowledge of the interactions between helminth glycans and host immunity and highlight the gaps in our understanding which are relevant to advancing therapeutics, vaccine development, and diagnostics.

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Vaccination-induced IgG response to Galα1–3GalNAc glycan epitopes in lambs protected against Haemonchus contortus challenge infection

Cited by 37 publications

References 33 publications

Galactosylated Fucose Epitopes in Nematodes

Galactosylated Fucose Epitopes in Nematodes

Intranasal Immunization of Lambs with Serine/Threonine Phosphatase 2A against Gastrointestinal Nematodes

Glycoconjugates in Host-Helminth Interactions

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