Vaccination as a control strategy against Salmonella infection in pigs: A systematic review and meta-analysis of the literature

Cruz, Maria Luisa de la; Conrado, I.; Nault, Andre; Pérez, Andrés; Domı́nguez, Lucas; Álvarez, Julio

doi:10.1016/j.rvsc.2017.03.005

Cited by 48 publications

(33 citation statements)

References 51 publications

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“…Increasing the quality of colostrum (i.e. the amount of immunoglobulins) through vaccination of pregnant sows before farrowing should be considered another potential strategy to protect suckling piglets from infection [43, 45–47] and even Salmonella shedding in older pigs [48, 49].…”

Section: Discussionmentioning

confidence: 99%

Weaned piglets: another factor to be considered for the control of Salmonella infection in breeding pig farms

Casanova-Higes

Marín-Alcalá

Andrés-Barranco

et al. 2019

Vet Res

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Field studies on Salmonella infection in suckling piglets are scarce due to the intrinsic difficulties of collecting proper samples (i.e. tonsils or mesenteric lymph nodes), and most of them rely on the analysis of rectal swabs that limit their accuracy. We used 495 slaughtered 4-weeks-old male piglets intended for human consumption from 5 Salmonella -seropositive breeding farms to collect gastrointestinal packages and perform a thorough detection of Salmonella on mesenteric lymph nodes and intestinal content. The overall prevalence of both infection and shedding was high (≈ 36%) indicating that piglets played an active role in Salmonella maintenance in the farms. Major serotypes found in piglets included 4,[5],12:i: (35.4%), Rissen (17.1%), Derby (10.9%) and Bovismorbificans (10.3%). In most of the infected animals (72.8%) the same serotype was found in mesenteric lymph nodes and feces. Significant higher ELISA OD% values were found in meat juice samples from non-infected piglets compared to infected ones (median OD% of 12.0 and 17.3, respectively; P = 0.002) suggesting some protective effect of sow’s colostrum. Salmonella was also isolated from feces from weaned sows contemporary of the slaughtered piglets, and 89% of the serotypes identified in sows were also detected in piglets. Pulsed field gel electrophoresis analyses showed that 75% of the piglet isolates that were compared to those of sows were related to them, suggesting the circulation of Salmonella strains between sows and piglets. It appears that improving piglet colostrum intake along with the reduction of the shedding in sows may favor the control of Salmonella infection in breeding farms.

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Section: Discussionmentioning

confidence: 99%

Weaned piglets: another factor to be considered for the control of Salmonella infection in breeding pig farms

Casanova-Higes

Marín-Alcalá

Andrés-Barranco

et al. 2019

Vet Res

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“…It is nowadays widely accepted that vaccination for Salmonella in modern pig production can play an important role in the intervention in high prevalence herds [7][8][9][10]32]. In a previous study, we constructed a novel live attenuated Salmonella Enteritidis vaccine candidate, named ΔXIII, and showed its efficacy against Salmonella Typhimurium in a vaccination-challenge analysis carried out in BALBc mice [18].…”

Section: Discussionmentioning

confidence: 99%

“…At the pre-harvest level, measures can be addressed to the prevention of introduction of Salmonella into the herd; the prevention of in-herd transmission; and the increase of resistance to infection [6,7]. In this regard, Salmonella vaccines are currently regarded as an adjunct to other on-farm control measures [7][8][9][10], by helping to prevent Salmonella colonization and the development of a carrier state, characteristic of pigs colonized by non-adapted serovars, such as S. Enteritidis and S. Typhimurium [7,11].…”

Section: Introductionmentioning

confidence: 99%

A DIVA vaccine strain lacking RpoS and the secondary messenger c-di-GMP for protection against salmonellosis in pigs

Gil¹,

Latasa²,

García-Ona³

et al. 2020

Vet Res

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Salmonellosis is the second most common food-borne zoonosis in the European Union, with pigs being a major reservoir of this pathogen. Salmonella control in pig production requires multiple measures amongst which vaccination may be used to reduce subclinical carriage and shedding of prevalent serovars, such as Salmonella enterica serovar Typhimurium. Live attenuated vaccine strains offer advantages in terms of enhancing cell mediated immunity and allowing inoculation by the oral route. However, main failures of these vaccines are the limited cross-protection achieved against heterologous serovars and interference with serological monitoring for infection. We have recently shown that an attenuated S. Enteritidis strain (ΔXIII) is protective against S. Typhimurium in a murine infection model. ΔXIII strain harbours 13 chromosomal deletions that make it unable to produce the sigma factor RpoS and synthesize cyclic-di-GMP (c-di-GMP). In this study, our objectives were to test the protective effects of ΔXIII strain in swine and to investigate if the use of ΔXIII permits the discrimination of vaccinated from infected pigs. Results show that oral vaccination of pre-weaned piglets with ΔXIII cross-protected against a challenge with S. Typhimurium by reducing faecal shedding and ileocaecal lymph nodes colonization, both at the time of weaning and slaughter. Vaccinated pigs showed neither faecal shedding nor tissue persistence of the vaccine strain at weaning, ensuring the absence of ΔXIII strain by the time of slaughter. Moreover, lack of the SEN4316 protein in ΔXIII strain allowed the development of a serological test that enabled the differentiation of infected from vaccinated animals (DIVA).

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“…This disease is commonly found in animals with concurrent debilitating infections, or if raised in environments that facilitate exposure to high doses of the pathogen, such as modern intensive rearing systems [4,5]. While avirulent pig vaccines for S. enterica serovars are available, vaccination is not recommended in pigs as carriers can still shed the bacteria and infect humans [6,7]. Prevention and control are mainly based on disinfection of premises, biosecurity measures and mass treatment with antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Putting the microbiota to work: Epigenetic effects of early life antibiotic treatment are associated with immune-related pathways and reduced epithelial necrosis following Salmonella Typhimurium challenge in vitro

et al. 2020

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Salmonella enterica serovar Typhimurium is an animal welfare and public health concern due to its ability to parasite livestock and potentially contaminate pork products. To reduce Salmonella shedding and the risk of pork contamination, antibiotic therapy is used and can contribute to antimicrobial resistance. Here we hypothesized that immune system education by the microbiota can play a role in intestinal resilience to infection. We used amoxicillin (15mg/Kg) to modulate the intestinal microbiome of 10 piglets, paired with same age pigs that received a placebo (n = 10) from 0 to 14 days of age. Animals were euthanized at 4weeks old. Each pig donated colon sections for ex vivo culture (n = 20 explants/pig). Explants were inoculated with S. Typhimurium, PBS or LPS (n = 6 explants/pig/group, plus technical controls). The gut bacteriome was characterized by sequencing of the 16S rRNA at 7, 21 days of age, and upon in vitro culture. Explants response to infection was profiled through high-throughput mRNA sequencing. In vivo antibiotic treatment led to β-diversity differences between groups at all times (P<0.05), while α-diversity did not differ between amoxicillin and placebo groups on day 21 and at euthanasia (P<0.03 on day 7). Explant microbiomes were not different from in vivo. In vitro challenge with S. Typhimurium led to lower necrosis scores in explants from amoxicillin-treated pigs, when compared to explants placebo-treated pigs (P<0.05). This was coupled with the activation of immune-related pathways in explants from amoxicillin-treated pigs (IL-2 production, NO production, BCR activation), when compared to placebo-treated pigs. In addition, several DNA repair and intestinal wound healing pathways were also only activated in explants from amoxicillin-treated pigs. Taken together, these findings suggest that immune education by the amoxicillin-disturbed microbiota may have contributed to mitigate intestinal lesions following pathogen exposure.

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Vaccination as a control strategy against Salmonella infection in pigs: A systematic review and meta-analysis of the literature

Cited by 48 publications

References 51 publications

Weaned piglets: another factor to be considered for the control of Salmonella infection in breeding pig farms

Weaned piglets: another factor to be considered for the control of Salmonella infection in breeding pig farms

A DIVA vaccine strain lacking RpoS and the secondary messenger c-di-GMP for protection against salmonellosis in pigs

Putting the microbiota to work: Epigenetic effects of early life antibiotic treatment are associated with immune-related pathways and reduced epithelial necrosis following Salmonella Typhimurium challenge in vitro

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