Vaccination against ticks (Boophilus spp.): the experience with the Bm86-based vaccine Gavac™

Fuente, José de la; Rodrı́guez, Manuel; Montero, Carlos; Redondo, María C.; García-García, Jose Carlos; Méndez, Luis; Serrano, E.; Valdés, Mario; Enríquez, A.; Canales, Mario; Ramos, Eduardo Antônio Gonçalves; Boué, Oscar; Machado, Humberto; Lleonart, Ricardo

doi:10.1016/s1050-3862(99)00018-2

Cited by 164 publications

(102 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One anti-tick vaccine has even been commercialized. However, its efficiency is limited and it is generally used in combination with acaricides [31].…”

Section: Discussionmentioning

confidence: 99%

Protective immunity against Ixodes ricinus induced by a salivary serpin

Prévôt

Couvreur

Denis

et al. 2007

Vaccine

View full text Add to dashboard Cite

Iris is a specific elastase inhibitor expressed in the salivary glands of the hard tick Ixodes ricinus. It belongs to the superfamily of serpins and interferes with both haemostasis and the immune response of the host. In this study, we first show that Iris is expressed in nymphs but not in the female midgut nor in males. We also show that Iris is present in the saliva. To examine its potency as anti-tick vaccine candidate, we set up three models of I. ricinus infestation on immunized animals: nymphs on mice, and adults and nymphs on rabbits. We report the rise of neutralizing antibodies following immunization of rabbits and mice. This comes with a significant protective immunity against ticks in rabbits only, resulting in a 30% mortality rate and a diminution of weight gain in both nymphs and adults and a prolongation of blood feeding time in adults. This is the first report on an anti-tick vaccine trial on I. ricinus using a protein able to interact with both host immunity and haemostasis, as a vaccinating antigen.

show abstract

“…One anti-tick vaccine has even been commercialized. However, its efficiency is limited and it is generally used in combination with acaricides [31].…”

Section: Discussionmentioning

confidence: 99%

Protective immunity against Ixodes ricinus induced by a salivary serpin

Prévôt

Couvreur

Denis

et al. 2007

Vaccine

View full text Add to dashboard Cite

show abstract

“…This is globally distributed with an important presence in Asia, parts of Australia, Madagascar, Southeastern Africa, the Caribbean, South and Central America and Mexico [49,50]. Tick saliva contains numerous molecules like sphingomyelinase-D (SMD) that might modulate host immune responses [51] and combined with other proteins has been proposed as blood meal strategy for the tick [52].…”

Section: Introductionmentioning

confidence: 99%

Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli

et al. 2014

View full text Add to dashboard Cite

Background: Inclusion bodies (IBs) are aggregated proteins that form clusters when protein is overexpressed in heterologous expression systems. IBs have been considered as non-usable proteins, but recently they are being used as functional materials, catalytic particles, drug delivery agents, immunogenic structures, and as a raw material in recombinant therapeutic protein purification. However, few studies have been made to understand how culture conditions affect the protein aggregation and the physicochemical characteristics that lead them to cluster. The objective of our research was to understand how pH affects the physicochemical properties of IBs formed by the recombinant sphingomyelinase-D of tick expressed in E. coli BL21-Gold (DE3) by evaluating two pH culture strategies.

show abstract

“…microplus vaccine products in other parts of the world. The level of efficacy reported for vaccines containing a recombinant form of Bm86 varies according to, among other factors, the expression system used for vaccine production, characteristics of the tick population targeted, and host factors (DE LA FUENTE et al, 1999;PATARROYO et al, 2002;RAND et al, 1989;RODRÍGUEZ et al, 1995;SITTE et al, 2002;ANDREOTTI et al, 2008;PARIZI et al, 2009). It has been postulated that sequence variation in the bm86 locus is associated with low susceptibility of certain R.…”

Section: Introductionmentioning

confidence: 99%

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Cunha

León

Pinto

et al. 2012

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The southern cattle fever tick, Rhipicephalus (Boophilus) microplus, is no doubt the most economically important ectoparasite of cattle globally. The inappropriate use of chemical acaricides has driven the evolution of resistance in populations of R. (B.) microplus. Anti-tick vaccines represent a technology that can be combined with acaricides in integrated control programs to mitigate the impact of R. (B.) microplus. The recombinant form of Bm86 antigen from the Campo Grande (rBm86-CG) strain of R. (B.) microplus was produced using the Pichia pastoris expression system to test its ability to immunoprotect cattle against tick infestation. Secretion of rBm86-CG by P. pastoris through the bioprocess reported here simplified purification of the antigen. A specific humoral immune response was detected by ELISA in vaccinated cattle. Immunoblot results revealed that polyclonal antibodies from vaccinated cattle recognized a protein in larval extracts with a molecular weight corresponding to Bm86. The rBm86-CG antigen showed 31% efficacy against the Campo Grande strain of R. (B.) microplus infesting vaccinated cattle. The rBm86-CG is an antigen that could be used in a polyvalent vaccine as part of an integrated program for the control of R. (B.) microplus in the region that includes Mato Grosso do Sul.Keywords: Tick, Rhipicephalus (Boophilus) microplus, anti-tick vaccine, recombinant Bm86, Pichia pastoris. ResumoO carrapato Rhipicephalus (Boophilus) microplus é, sem dúvidas, o ectoparasito economicamente mais importante para o gado a nível mundial. A utilização inadequada de acaricidas tem impulsionado a evolução da resistência em populações de R. (B.) microplus. Vacinas contra o carrapato representam uma tecnologia que pode ser combinada com acaricidas em programas de controle integrado para diminuir o impacto de R. (B.) microplus. A forma recombinante da Bm86 da cepa Campo Grande (rBm86-CG) de R. (B.) microplus foi produzido utilizando o sistema de expressão em Pichia pastoris para testar sua capacidade de imunoproteção ao gado contra a infestação de carrapatos. A secreção de rBm86-CG em P. pastoris pelo bioprocesso, simplificou a purificação do antígeno. A resposta imune humoral específica foi detectada por ELISA em soros de bovinos vacinados. Resultados de "imunoblot" revelaram que anticorpos policlonais de bovinos vacinados reconheceram uma proteína em extratos de larvas com um peso molecular correspondente à Bm86. O antígeno rBm86-CG mostrou eficácia de 31% contra a amostra CG de R. (B.) microplus utilizada para infestar os bovinos vacinados. Pelos resultados obtidos, concluímos que a rBm86-CG é um antígeno que pode ser usado em uma vacina polivalente, como parte de um programa integrado para o controle de R. (B.) microplus no estado do Mato Grosso do Sul, Brasil.

show abstract

Vaccination against ticks (Boophilus spp.): the experience with the Bm86-based vaccine Gavac™

Cited by 164 publications

References 19 publications

Protective immunity against Ixodes ricinus induced by a salivary serpin

Protective immunity against Ixodes ricinus induced by a salivary serpin

Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Contact Info

Product

Resources

About