Vaccination against tick-borne encephalitis virus, a flavivirus, prevents disease but not infection, although viremia is undetectable

Kreil, Thomas R.; Maier, Elisabeth; Fraiss, Sabine; Attakpah, Eva; Burger, Ingrid; Mannhalter, Josef W.; Eibl, Martha M.

doi:10.1016/s0264-410x(98)80102-3

Cited by 22 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Neutralizing antibodies are long-lasting following natural infection and protection against disease on re-exposure is complete, although re-exposure often is accompanied by a boost in antibody indicating limited infection. Studies employing passive transfer of polyclonal immune serum or monoclonal antibodies to Japanese encephalitis (JE) (10, 26–28), West Nile (WN) (16, 29–31), tick-borne encephalitis (TBE) (32–35) and YF (36,37) demonstrated that nAbs against the envelope protein confers protective immunity against virus challenge.…”

Section: Discussionmentioning

confidence: 99%

Immune correlates of protection against yellow fever determined by passive immunization and challenge in the hamster model

Julander

Trent

Monath

2011

Vaccine

View full text Add to dashboard Cite

Live, attenuated yellow fever (YF) 17D vaccine is highly efficacious but causes rare, serious adverse events resulting from active replication in the host and direct viral injury to vital organs. We recently reported development of a potentially safer β-propiolactone-inactivated whole virion YF vaccine (XRX-001) which was highly immunogenic in mice, hamsters, monkeys, and humans (Vaccine 2010; 28:3827–40; New Engl J Med 2011;364:1326–33). To characterize the protective efficacy of neutralizing antibodies stimulated by the inactivated vaccine, graded doses of serum from hamsters immunized with inactivated XRX-001 or live 17D vaccine were transferred to hamsters by the intraperitoneal (IP) route 24 hours prior to virulent, viscerotropic YF virus challenge. Neutralizing antibody (PRNT50) titers were determined in the sera of treated animals 4 hours before challenge and 4 and 21 days after challenge. Neutralizing antibodies were shown to mediate protection. Animals having 50% plaque reduction neutralization test (PRNT50) titers of ≥40 four hours before challenge were completely protected from disease as evidenced by viremia, liver enzyme elevation, and protection against illness (weight change) and death. Passive titers of 10–20 were partially protective. Immunization with the XRX-001 vaccine stimulated YF neutralizing antibodies that were equally effective (based on dose response) as antibodies stimulated by live 17D vaccine. The results will be useful in defining the level of seroprotection in clinical studies of new yellow fever vaccines.

show abstract

Section: Discussionmentioning

confidence: 99%

Immune correlates of protection against yellow fever determined by passive immunization and challenge in the hamster model

Julander

Trent

Monath

2011

Vaccine

View full text Add to dashboard Cite

show abstract

“…Cross-reactions due to family-wide envelope protein epitopes may occur when classic techniques such as hemagglutination-inhibition (HI), complement fixation (CF) and immunofluorescence [8][9][10] are used. For example, individuals vaccinated against yellow fever have yielded false-positive results in HI tests or in a commercially produced enzyme-linked immunosorbent assay (ELISA) for antibody to tickborne encephalitis (Immuno AG, Vienna, Austria; unavailable in the United States).…”

Section: Introductionmentioning

confidence: 99%

Enzootic transmission of deer tick virus in New England and Wisconsin sites.

Ebel¹,

Campbell²,

Goethert³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. To determine whether rodents that are intensely exposed to the deer tick-transmitted agents of Lyme disease, human granulocytic ehrlichiosis, and human babesiosis are also exposed to deer tick virus (DTV), we assayed serum samples from white-footed mice (Peromyscus leucopus) and meadow voles (Microtus pennsylvanicus) in sites densely infested by deer ticks. To conduct serosurveys, we developed an enzyme-linked immunosorbent assay (ELISA) and Western blot assay by cloning, expressing, and purifying a portion of the DTV envelope glycoprotein (DTV rE) for use as test antigen. Sera from mice and voles trapped in Massachusetts, Rhode Island, and Wisconsin were screened by ELISA for IgG reactive to DTV rE. Samples that were positive or borderline by ELISA were subsequently analyzed by immunoblotting. Samples reactive in both assays were considered to be positive. Three percent of 264 mouse samples collected from sites in Rhode Island, 3.8% of 52 samples from mice trapped in Wisconsin, and 3.9% of 282 samples collected from mice trapped on Nantucket Island, MA were positive. No samples from either Great Island, MA, or voles from any study site were reactive. A reverse transcriptase-polymerase chain reaction yielded molecular evidence of DTV infecting questing adult deer ticks in sites where seroreactive mice were trapped, but not from ticks collected where serologic evidence of virus perpetuation was absent. White-footed mice appear to be exposed to DTV in certain sites where other deer tick-borne agents perpetuate. This virus may be maintained in the same enzootic cycle.

show abstract

“…Так, в исследовании T.R. Kreil et al [33] мышей вна-чале иммунизировали вакциной FSME-Immune (по схеме, трижды) и затем заражали летальной дозой ВКЭ. Как и ожидалось, после иммуни-зации индуцировались высокие титры антител против белка E, что приводило к 100% выжива-емости.…”

Section: крылова нв Medical Immunology (Russia)/meditsinskaya Immununclassified

Formation of Innate and Adaptive Immune Response Under the Influence of Different Flavivirus Vaccines

Владимировна¹

2015

Med. immunol.

View full text Add to dashboard Cite

Vaccination against tick-borne encephalitis virus, a flavivirus, prevents disease but not infection, although viremia is undetectable

Cited by 22 publications

References 17 publications

Immune correlates of protection against yellow fever determined by passive immunization and challenge in the hamster model

Immune correlates of protection against yellow fever determined by passive immunization and challenge in the hamster model

Enzootic transmission of deer tick virus in New England and Wisconsin sites.

Formation of Innate and Adaptive Immune Response Under the Influence of Different Flavivirus Vaccines

Contact Info

Product

Resources

About