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Comparative study of reproduction of sindbis virus sensitive and resistant to adamantane derivatives

“…ACAA is an effective selective inhibitor of SV reproduction [1], it effectively protects cells from death in a wide range of concentrations: for CDs0 ACAA is 191.25 pg/ml and IDs0=l 1.25 pg/ml (for remantadine CDs0 51.25 ~tg/ml and ID50=21.25 /ag/ml; CDs0 is the concentration of the compound at which 50% cells survive and IDs0 is the concentration protecting 50% infected cells from death).…”

“…SV strain Ag 339 was from Virus Museum Laboratory of the Institute. Resistant SV strain was obtained by serial passages with ascending ACAA concentrations [1].…”

“…Antiviral activity of the test compounds was evaluated routinely by the decrease in infective titer [1]. The development of virus-specific cytopathic effect in infected cells was evaluated using trypan blue staining [ 1] (multiplicity of infection (MI) of plaque-forming units (PFU)/cell).…”

“…The development of virus-specific cytopathic effect in infected cells was evaluated using trypan blue staining [ 1] (multiplicity of infection (MI) of plaque-forming units (PFU)/cell).…”

“…Serial passages of SV with 1-adamantane carbonic acid amide (ACAA), the most potent antiviral drug, resulted in derivation of resistant SV variant [1]. In this study we compared characteristics of resistant and original sensitive variants of the virus.…”