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Хидырова, Н. К.; Mamatkulova, N. M.; Kiktev, M. M.; Shakhidoyatov, Kh. M.

doi:10.1023/a:1017571910675

Cited by 3 publications

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“…Flowers of the plant are used in medicine to ameliorate coughing. Various aqueous decoctions and infusions of the plant are used as mucosal, softening, binding, and anti-inflammatory agents [6].Alcohol extracts of the dried and ground plant leaves were obtained by the literature method [7] in yields of 22.5 and 23.2%, respectively. The alcohol extracts were condensed to 1/3 the volume and worked up with weak NaOH solution in order to remove free acids.…”

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Neutral Constituents of Alcea rosea Leaves

et al. 2015

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Plants of the family Malvaceae are distinguished among leafy plants by high contents of polyprenols in the aerial parts [1][2][3][4]. In continuation of research on polyisoprenoids from plants of this family, we report the polyisoprenoid compositions of two varieties of Alcea rosea L. (rose hollyhock, 1, and ruby hollyhock, 2) growing in Samarkand Province. Plant material was collected on Aug. 26, 2011 during fruiting in Pai Aryk District.Alcea rosea L. is a beautiful garden flower that numbers ~60 varieties distributed in the eastern Mediterranean region, the Middle East, and Central Asia [5]. Flowers of the plant are used in medicine to ameliorate coughing. Various aqueous decoctions and infusions of the plant are used as mucosal, softening, binding, and anti-inflammatory agents [6].Alcohol extracts of the dried and ground plant leaves were obtained by the literature method [7] in yields of 22.5 and 23.2%, respectively. The alcohol extracts were condensed to 1/3 the volume and worked up with weak NaOH solution in order to remove free acids. The residue was saponified as before [8] to isolate total neutral substances (NS) in yields of 8.5% [1, with polyprenol (PP) content 84.7%] and 8.25% (2, with PP content 80.7%) of the air-dried mass (ADM). Then, column chromatography separated the obtained total NS from A. rosea leaves. The adsorbent was KSK silica gel (60-100 mesh); mobile phase, petroleum ether-Et 2 O mixtures (50:1, 30:1, 25:1). Fractions (100 mL) were collected and monitored by TLC. Identical fractions were combined. PP fractions were isolated. The PP yield for 1 was 1.88% (of ADM); for 2, 1.76%. Furthermore, hydrocarbon, triterpenoid, and phytol fractions were isolated. These were identified by preparative TLC as before [9]. PP fractions were analyzed using an Agilent 1100 chromatograph with an Eclipse XDB-C-18 column (0.46 u 150 mm) with UV detection at 210 nm. The mobile phase was a gradient (%B); 0-20 min, 0-75; 20-25 min, 75-100; 25-30 min, 100-0 at flow rate 0.75 mL/min. The analysis time was 30 min. The mobile-phase gradient consisted of systems A (MeOH-H 2 O, 9:1, v/v) and B (MeOH-hexane-i-PrOH, 2:1:1) [9]. PP from cotton leaves with a known composition were used as standards [10]. Table 1 presents the HPLC analytical data. Table 1 shows the constituent ratio of the rose and ruby hollyhock leaf fractions in addition to the PP in each sample. It can be seen that undecaprenol (n = 11) and dodecaprenol (n = 12) dominated the PP-homologs. The decaprenol (n = 10) content in rose hollyhock was 9.4%; in ruby, 8.2%. The tridecaprenol fractions were identical at 8.6%.The other constituents of NS from A. rosea (rose hollyhock) were identified on an Agilent 7890 GC-5975 without additional separation by comparing their retention times with those of known compounds and comparing the mass spectra with hose in the NIST 02MS electronic library. The accuracy of identification was 75-90%. Table 2 presents the results. Table 2 shows that the principal components of the NS were E-sitosterol (20.9%), phytol (20.55%),...

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“…Alcohol extracts of the dried and ground plant leaves were obtained by the literature method [7] in yields of 22.5 and 23.2%, respectively. The alcohol extracts were condensed to 1/3 the volume and worked up with weak NaOH solution in order to remove free acids.…”

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Neutral Constituents of Alcea rosea Leaves

et al. 2015

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“…In particular, the preparation gamapren (OOO GamaVetFarm, Russia) based on moraprenols is manufactured by phosphorylation of polyprenols isolated from Morus spp. The preparation exhibits antiviral action against Herpes viruses by suppressing the synthesis of viral proteins and stimulating the production of interferons [4].In continuation of research on plant polyprenols [5][6][7][8] and in order to compare the quantitative contents of M. alba and M. nigra polyprenols, we present results from a study of polyisoprenols from these two mulberry varieties growing in Tashkent Oblast.Total polyisoprenoids were isolated by the previously published method [9]. Dried and ground leaves (10 g) produced total polyisoprenoids in 2.6 and 3.2% yields.…”

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“…Standard samples of D-tocopherol and sitosterol were obtained from cotton leaves. Standard samples were prepared as described before [9]. Table 1 presents the results.…”

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Polyisoprenoids from leaves of Morus alba and M. nigra

et al. 2012

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Trees or bushes of the genus Morus (mulberry) have been cultivated since antiquity. Mainly two species grow in Uzbekistan, M. alba and M. nigra. These species also include little studied varieties such as khasak-tut (used to feed silk worms), balkhi-tut (for fruit), ilon-tut and plakuchaya (as decorative plants), and shotut (M. nigra, for fruit) [1].Three polyisoprenoid alcohols, undeca-and dodecaprenols and solanesol, were isolated from silk-worm cocoons and leaves of M. alba [2]. Solanesol was not observed in the leaves. Moraprenols (polyprenols) from leaves of M. alba growing in Fergan Oblast were studied [3]. However, the quantitative composition of the polyprenologs was not reported. Polyprenols from M. nigra have not been reported.Recently a rather large number of preparations based on the use of polyprenols has appeared. In particular, the preparation gamapren (OOO GamaVetFarm, Russia) based on moraprenols is manufactured by phosphorylation of polyprenols isolated from Morus spp. The preparation exhibits antiviral action against Herpes viruses by suppressing the synthesis of viral proteins and stimulating the production of interferons [4].In continuation of research on plant polyprenols [5][6][7][8] and in order to compare the quantitative contents of M. alba and M. nigra polyprenols, we present results from a study of polyisoprenols from these two mulberry varieties growing in Tashkent Oblast.Total polyisoprenoids were isolated by the previously published method [9]. Dried and ground leaves (10 g) produced total polyisoprenoids in 2.6 and 3.2% yields. The content of total polyprenols was 1.37 and 1.97%, respectively, of the airdried leaf mass. The qualitative and quantitative compositions of the total polyisoprenoids were determined by high-performance TLC (HPTLC, Camag), by densitometry with UV detection at 210 nm, and by other alternate methods. HPTLC analysis used Sorbfil HPTLC-AF-UV plates (10 u 10). Plates were rinsed in distilled CHCl 3 and dried in air at 20-25°C for 10 min. The eluent was toluene:EtOAc (19:1). Compounds (1.0 mg) were dissolved in solvent (1 mL) and sampled (5 PL). The distance from the origin to the solvent front was 70.0 mm. The distance between tracks was 7.7 mm. Plates were dried in air at 20-25°C for 15 min.The standards were authentic samples of polyprenols from leaves of Asimina triloba (at least 96% pure) and those isolated by us from cotton leaves (at least 95% pure) of composition C 50 -C 65 . Standard samples of D-tocopherol and sitosterol were obtained from cotton leaves. Standard samples were prepared as described before [9]. Table 1 presents the results.According to the results, total polyisoprenoids contained polyprenols and D-tocopherol and sitosterol. The sitosterol contents in M. nigra and M. alba were almost identical. However, the amount of D-tocopherol was almost 13 times greater in leaves of M. nigra than in those of M. alba. The homologous composition of polyprenols from both M. nigra and M. alba included deca-, undeca-, and dodecaprenols with undecaprenol do...

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Growth-stimulating and anti-stress activity of lipids of the seed of Zygophyllum oxianum Boriss.under salt stress

Svetlana,

Rano,

Shahrukh

et al. 2023

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It is known that the lipid components of plant cells play an important role in protecting plants from the influence of stress factors. Changes in membrane lipids in response to salinity are observed in many plant species, including halophytes and glycophytes. Lipid messenger molecules are involved in many important processes of the adaptive mechanism that allow plants to cope with salt stress. The aim of the work was the studying of the lipids of the seeds of the plant Zygophyllum oxianum Boriss. (family of Zygophyllaceae) and their effect on the growth of wheat shoots grown under saline conditions, as well as the effect of plant lipids on the lipid composition of wheat seedlings. As a result of the study, we found that pre-sowing treatment of wheat and cucumber seeds with neutral components of Zygophyllum moxianum seed lipids in low doses stimulates the growth and development of seedlings of these crops under normal conditions and under the influence of salt stress, promotes the formation of total lipids and the biosynthesis of eicosatrienoic and arachidonic acids in wheat seedlings grown under saline conditions.

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Cited by 3 publications

References 1 publication

Neutral Constituents of Alcea rosea Leaves

Neutral Constituents of Alcea rosea Leaves

Polyisoprenoids from leaves of Morus alba and M. nigra

Growth-stimulating and anti-stress activity of lipids of the seed of Zygophyllum oxianum Boriss.under salt stress

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