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Varbanets, L.D.; Vasil'ev, V N; Brovarskaya, O. S.

doi:10.1023/a:1022261619561

Cited by 12 publications

(10 citation statements)

References 10 publications

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“…Within the detected glycoproteins, we observed heterogenous glycans decorating the proteins. The most abundant oligosaccharides detected were similar to the O antigen characterized in a number of R. solanacearum strains (Varbanets et al 2003). Varbanets et al (2003) detected N-acetyl glucosamine, rhamnose (deoxyhexose) and xylose ( pentose) in the O-antigens characterized.…”

Section: Discussionmentioning

confidence: 58%

“…The most abundant oligosaccharides detected were similar to the O antigen characterized in a number of R. solanacearum strains (Varbanets et al 2003). Varbanets et al (2003) detected N-acetyl glucosamine, rhamnose (deoxyhexose) and xylose ( pentose) in the O-antigens characterized. Our in silico analysis of R. solanacearum GMI1000 revealed a homologue of a rhamnosyltransferase (RSc0687) within a typical glycan biosynthetic cluster that also contain enzymes usually involved in the synthesis of the O antigen.…”

Section: Discussionmentioning

confidence: 58%

“…The majority of the glycopeptides were found modified with a pentasaccharide composed of HexNAc-(Pen)-dHex 3 , which is similar to the O antigen subunit characterized in the LPS structures of multiple R. solanacearum strains ( Figure 2A) (Varbanets et al 2003). However, the whole glycoproteome exhibited eight unique glycoforms (Figure 2, Supplementary data, Figure S3) ranging in size from a single HexNAc (N-acetylhexosamine) (Supplementary data, Figure S3A) to an octasaccharide composed of HexNAc-(Pen)-dHex 3 -Pen 3 (dHex; deoxyhexose, pen; pentose) (Supplementary data, Figure S3F).…”

Section: Pgll Rs Is Essential For O-glycosylation In R Solanacearummentioning

confidence: 89%

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Protein O-linked glycosylation in the plant pathogenRalstonia solanacearum

et al. 2015

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Ralstonia solanacearum is one of the most lethal phytopathogens in the world. Due to its broad host range, it can cause wilting disease in many plant species of economic interest. In this work, we identified the O-oligosaccharyltransferase (O-OTase) responsible for protein O-glycosylation in R. solanacearum. An analysis of the glycoproteome revealed that 20 proteins, including type IV pilins are substrates of this general glycosylation system. Although multiple glycan forms were identified, the majority of the glycopeptides were modified with a pentasaccharide composed of HexNAc-(Pen)-dHex(3), similar to the O antigen subunit present in the lipopolysaccharide of multiple R. solanacearum strains. Disruption of the O-OTase led to the total loss of protein glycosylation, together with a defect in biofilm formation and reduced pathogenicity towards tomato plants. Comparative proteomic analysis revealed that the loss of glycosylation is not associated with widespread proteome changes. Only the levels of a single glycoprotein, the type IV pilin, were diminished in the absence of glycosylation. In parallel, disruption of glycosylation triggered an increase in the levels of a surface lectin homologous to Pseudomonas PA-IIL. These results reveal the important role of glycosylation in the pathogenesis of R. solanacearum.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 58%

Section: Pgll Rs Is Essential For O-glycosylation In R Solanacearummentioning

confidence: 89%

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Protein O-linked glycosylation in the plant pathogenRalstonia solanacearum

et al. 2015

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“…[17] We thus considered (R)-3-hydroxymyristyl-ACP to be the methyl donor of the PhcB catalytic reaction. [20] Therefore, PhcB might use (R)-3-hydroxymyristyl-ACP to produce (R)-3-OH MAME.T his appears to be reasonable for the rapid synthesis of the phc QS signal when required. [19] Lipid Af rom several strains of R. solanacearum has been shown to contain 3-hydroxymyristic acid as the major acyl chain.…”

Section: Discussionmentioning

confidence: 99%

“…[19] Lipid Af rom several strains of R. solanacearum has been shown to contain 3-hydroxymyristic acid as the major acyl chain. [20] Therefore, PhcB might use (R)-3-hydroxymyristyl-ACP to produce (R)-3-OH MAME.T his appears to be reasonable for the rapid synthesis of the phc QS signal when required. However, we did not detect any apparent transmembrane domains or signal peptides in PhcB proteinso nu sing several prediction programsf or cellular localization; therefore, the correct localization of PhcB in R. solanacearum cells remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Methyl 3‐Hydroxymyristate, a Diffusible Signal Mediating phc Quorum Sensing in Ralstonia solanacearum

et al. 2015

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Ralstonia solanacearum, a plant pathogenic bacterium causing "bacterial wilt" on crops, uses a quorum sensing (QS) system consisting of phc regulatory elements to control its virulence. Methyl 3-hydroxypalmitate (3-OH PAME) was previously identified as the QS signal in strain AW1. However, 3-OH PAME has not been reportedly detected from any other strains, and this suggests that they produce another unknown QS signal. Here we identify (R)-methyl 3-hydroxymyristate [(R)-3-OH MAME] as a new QS signal that regulates the production of virulence factors and secondary metabolites. (R)-3-OH MAME was synthesized by the methyltransferase PhcB and sensed by the histidine kinase PhcS. The phylogenetic trees of these proteins from R. solanacearum strains were divided into two groups, according to their QS signal types--(R)-3-OH MAME or (R)-3-OH PAME. These results demonstrate that (R)-3-OH MAME is another crucial QS signal and highlight the unique evolution of QS systems in R. solanacearum.

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Overexpression of an apple broad range agglutinating lectin does not promote in planta resistance to fire blight and bacterial wilt

Bodelot,

Chavonet,

Brisset

et al. 2023

J Plant Pathol

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Untitled

Cited by 12 publications

References 10 publications

Protein O-linked glycosylation in the plant pathogenRalstonia solanacearum

Protein O-linked glycosylation in the plant pathogenRalstonia solanacearum

Methyl 3‐Hydroxymyristate, a Diffusible Signal Mediating phc Quorum Sensing in Ralstonia solanacearum

Overexpression of an apple broad range agglutinating lectin does not promote in planta resistance to fire blight and bacterial wilt

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