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Murad, M. M.

doi:10.1023/a:1022567919705

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…The thermal phase transition of the PNIPAM solution in the microchannel-microheater chip was also applied to controlling the concentration/accumulation of a trace amount of an analyte by the switching on/off of a bias voltage to the heater. As a water-soluble hydrophobic dye, we chose AO because AO shows strong fluorescence in hydrophobic environments while being a weak emitter in water . In the actual experiments, an aqueous PNIPAM solution (0.25 wt %) containing AO (1.0 × 10 -3 M) was introduced to the chip at ν = 2 μL/min, and the fluorescence from AO was monitored by the switching on/off of V .…”

Section: Resultsmentioning

confidence: 99%

Thermal Phase Transition of an Aqueous Poly(N-isopropylacrylamide) Solution in a Polymer Microchannel-Microheater Chip

et al. 2003

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Plastic microchannel-microheater chips were fabricated on the basis of imprinting and photolithography/etching techniques. Knowing the temperature-dependent fluorescence lifetime of Rhodamine B in water, the temperature profile of the solution phase in the microchannel in the vicinity of the heater was evaluated by space- and time-resolved fluorescence microspectroscopies. The fabricated chip was then applied to control the thermal phase transition of an aqueous poly(N-isopropylacrylamide) (PNIPAM) solution. PNIPAM particle formation by the phase transition was controlled by both the applied voltage to the heater and the solution-flow rate. The temperature profile showing below or above the phase-transition temperature (i.e., 32 °C) and solution-flow profile in the microchannel were visualized successfully on the basis of the PNIPAM particle formation. The continuous and automated concentration of a hydrophobic solute by the PNIPAM particles produced by the phase transition was also explored under solution-flow conditions by using the fabricated chip.

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Section: Resultsmentioning

confidence: 99%

Thermal Phase Transition of an Aqueous Poly(N-isopropylacrylamide) Solution in a Polymer Microchannel-Microheater Chip

et al. 2003

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“…2, N-10-tetradecyl acridine orange (C14-AO) and n-10-octadecyl acridine orange (C18-AO) were synthesized by the reaction of acridine orange with 1-bromotetradecane and 1-bromooctadecane, respectively [13][14][15][16]. Toluene was used as a solvent for synthesis and the alkyl bromide was added drop-wise over a 1-h time span to the acridine orange.…”

Section: Synthesismentioning

confidence: 99%

Comparison of N-alkyl acridine orange dyes as fluorescence probes for the determination of cardiolipin

Kaewsuya

Miller

Danielson

et al. 2008

Analytica Chimica Acta

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“…[9][10][11] Lateral diffusion is measured very accurately by either fluorescence recovery after photobleaching [4][5][6][7][8][12][13][14] or fluorescence correlation spectroscopy, [15][16][17][18] where the latter avoids perturbing the sample. The interpretation of the results is most straightforward when there is a single diffusing species; however, the data can, in principle, be analyzed for multiple species.…”

Section: Introductionmentioning

confidence: 99%

“…There has been a considerable amount of interest in the lateral diffusion of adsorbates at chemical interfaces for a variety of important systems, including biological membranes, artificial membranes, , chromatographic interfaces, − and liquid−liquid interfaces. − Lateral diffusion is measured very accurately by either fluorescence recovery after photobleaching − ,− or fluorescence correlation spectroscopy, − where the latter avoids perturbing the sample. The interpretation of the results is most straightforward when there is a single diffusing species; however, the data can, in principle, be analyzed for multiple species. , …”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Study of an Adsorbed Oligonucleotide Undergoing Both Lateral Diffusion and Strong Adsorption

Wirth

Swinton

2001

J. Phys. Chem. B

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Single-molecule fluorescence spectroscopy was used to probe the lateral transport of an adsorbed oligonucleotide, the SP6 promoter primer, which is a 24-mer labeled at the 5′ end with tetramethylrhodamine. The oligonucleotide was adsorbed to the interface of an aqueous solution of 0.01 M KCl and silica chemically modified by chlorodimethyloctadecylsilane. Confocal fluorescence microscopy achieved single-molecule resolution, with a molecule in the beam 7% of the time. Autocorrelation of the data fit well to a model having two species, one diffusing and the other one undergoing transient strong adsorption. A small number of bursts, 0.3%, had unusually long durations, accounting for the slow component in the autocorrelation. When these long bursts were excised from the data, the autocorrelation fit well to simple diffusion, with D ) 4 × 10 -6 cm 2 /s. Autocorrelation of the long bursts alone gave a rate constant for desorption of 3 s -1 . The strongly adsorbed molecules were found to comprise 10% of the total population of adsorbates. It is concluded that the lateral transport of the SP6 promoter primer is described by fast lateral diffusion interrupted by rare, reversible, strong adsorption to defect sites. During strong adsorption, the tetramethylrhodamine label undergoes hindered motion, suggesting it is not the adsorbing moiety.

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Cited by 11 publications

References 14 publications

Thermal Phase Transition of an Aqueous Poly(N-isopropylacrylamide) Solution in a Polymer Microchannel-Microheater Chip

Thermal Phase Transition of an Aqueous Poly(N-isopropylacrylamide) Solution in a Polymer Microchannel-Microheater Chip

Comparison of N-alkyl acridine orange dyes as fluorescence probes for the determination of cardiolipin

Single-Molecule Study of an Adsorbed Oligonucleotide Undergoing Both Lateral Diffusion and Strong Adsorption

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