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Allagulova, Ch.R.; Gimalov, F. R.; Шакирова, Ф. М.; Вахитов, В. А.

doi:10.1023/a:1026077825584

Cited by 202 publications

(70 citation statements)

References 73 publications

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“…Dehydrins act as chaperons, stabilizing vesicles, proteins, and membrane structures in stressed plants (Close, 1997;Allagulova et al, 2010). However, the precise function of these proteins remains unknown.…”

Section: Differential Expression Of Sccbf1 Pyrroline-5-carboxylate Smentioning

confidence: 99%

“…Studies in wheat have reported an association between the CBF genes and LEA expression upon abiotic stress Kobayashi et al, 2005). Dehydrins like genes (DHNs) are members of the LEA protein family, and the most abundant class of dehydrins, such as the barley dehydrins DHN1, DHN2, DHN3, DHN4, DHN6, and DHN9, is induced in response to drought or ABA (Allagulova et al, 2010). Some studies in potato and tobacco have also demonstrated an association of CBF overexpression with LEA protein expression.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene

et al. 2013

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Cultivated potato crops are sensitive to drought stress, reducing yield and tuber quality when the soil water potential drops to -0.3 MPa. However, drought not only affects plant growth and physiological activity, but this stress also induces biochemical and molecular changes in the cellular gene expression profile, triggering genes that play a direct role in plant protection and gene regulation. The genes involved in regulation include C-repeat Binding Factors/Dehydration responsive element binding (CBF/DREB) transcription factors, which increase cold, drought and salt tolerance in different plant species. The aim of this research was to evaluate whether the overexpression of the ScCBF1 gene from Solanum commersonii enhances drought stress tolerance in transgenic Solanum tuberosum and S. commersonii plantlets grown in vitro and induces drought adaptation mechanisms, such as osmoprotectors and genes for osmotic adjustment and membrane stability. Drought conditions were simulated through the addition of polyethylene glycol (PEG4000) to hormone-free MS medium. The vegetative growth, root development, proline content, and ScCBF1, Δ1-pyrroline-5-carboxylate synthetase (P5CS) and Dehydrins like genes (DNH10) expression were evaluated. The constitutive in vitro overexpression of ScCBF1 in both potato species showed better overall plantlet growth and root development under drought stress and higher proline levels in the stems and leaves. A significant increase in DNH10 expression was also associated with drought stress. In summary, the expression of ScCBF1 in potatoes induces responses associated with drought adaptation mechanisms, resulting in better overall plant growth.

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Section: Differential Expression Of Sccbf1 Pyrroline-5-carboxylate Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene

et al. 2013

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“…Metabolic rates are lowered which create energy imbalance. Freezing (0°C and below) temperatures cause membrane injury [3], decrease the respiration rate and ATP content [4], resulting in poor germination, reduced seedling emergence, decreased seedling vigour, and ultimately severe loss in yield of the food crops of tropical and subtropical origins [5][6][7][8][9]. Embryos, excised from seed coats of soybeans, leak profusely during the first minutes of imbibition.…”

Section: Germination Physiology In Low Temperaturementioning

confidence: 99%

Engineering Germination Fitness for Sub-Physiological Temperatures in Plants

Javeed¹,

Cheng²,

Song³

et al. 2012

Int J Mol Biol

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Abstract-Germination is a key event in plant life cycle and imbibition temperature is an important factor for the major reorganization processes in the germinating seeds. Sub-physiological temperature at this stage affects virtually all aspects of cellular function including protein folding, kinetic parameters of membrane fluidity, protein assembly, and general metabolic processes. In this article we will review the research which has been carried out to decipher the basic mechanisms of low temperature (LT) stress with special emphasis on germination. With the better understanding of LT tolerance in some plant species we will also discuss how these attributes can be transferred in the important food crops to attain better germination stamina at sub-physiological temperatures. At germination stage, the cellular machinery in the embryo is not at its proper place and is going through reorganisation and any environmental severity has devastating effects on the fragile plant. But LT resistant species have evolved the ability to acclimatise, with the remodelling of cell and tissue structures and the reprogramming of metabolism and gene expression. Metabolic networks are redirected towards the synthesis of cryoprotectant molecules, which in association with other proteins bring about physical and biochemical restructuring of cell membranes through changes in the lipid composition and induction of other non-enzymatic proteins that alter the freezing point of water. Genetic engineering of crops for enhanced seed germination performance will certainly help to achieve optimum agricultural yields.. Germination physiology in low temperatureAll environmental stresses disrupt the normal functioning of a living system. To re-establish metabolic homeostasis, living systems require an adjustment of metabolic pathways in a process usually referred to as acclimation. For temperate plants, low temperature (LT) is a major non-living element of the environmental that affects water and nutrient uptake, membrane fluidity and protein and nucleic acid conformation, drastically influencing cellular metabolism directly by reducing the rates of biochemical reactions [1], accompanied by changes in the transcriptome, proteome and metabolome [2]. Chilling temperatures (0 to 15°C) frequently occur in nature and affect many species of plants, especially those of tropical origin (such as rice, corn, tomato and soybean), by causing wilting, chlorosis, or necrosis, thus restricting their growth and development. Metabolic rates are lowered which create energy imbalance. Freezing (0°C and below) temperatures cause membrane injury [3], decrease the respiration rate and ATP content [4], resulting in poor germination, reduced seedling emergence, decreased seedling vigour, and ultimately severe loss in yield of the food crops of tropical and subtropical origins [5][6][7][8][9]. Embryos, excised from seed coats of soybeans, leak profusely during the first minutes of imbibition. In the embryos imbibed at 10°C or lower, disproportionately more solutes leak ...

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“…Most of dehydrins are associated mainly with the process of embryogenesis, but they can also be accumulated in vegetative parts during the plant lifecycle. Generally, dehydrins are enriched with glycine and lysine residues, but they lack cysteine and tryptophan (Allagulova et al, 2003). The characteristic feature of all dehydrins is conservative sequences denominated as K-, S-and Y-segmets which are their structural attribute.…”

Section: Introductionmentioning

confidence: 99%

PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490

Zimová¹,

Boszorádová²,

Gregorová³

et al. 2017

J microb biotech food sci

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This work is focused on preparation of a plant transformation vector pDH2 containing the expression unit of the dehydrin gene At2g21490 under a control of the constitutive dCaMV 35S promoter and the selectable marker neomycin phosphotransferase gene controlled by the nos promoter. The gene At2g21490 is classified as a dehydrin of the type K2SY2. The gene was isolated from Arabidopsis thaliana by PCR approach. The plasmid pDH2 was transformed into the strain Agrobacterium tumefaciens LBA 4404. The stability of the plasmid pDH2 in Agrobacterium has been verified by restriction analysis after re-transformation of the pDH2 into Escherichia coli.

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Cited by 202 publications

References 73 publications

Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene

Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene

Engineering Germination Fitness for Sub-Physiological Temperatures in Plants

PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490

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