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Chen, Jinfeng; Staub, Jack E.; Jiang, Jiming

doi:10.1023/a:1008603608879

Cited by 29 publications

(2 citation statements)

References 23 publications

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“…In Cucumis, studies involving molecular cytogenetics were mainly focused on important cultivated crops (e.g., cucumbers, melons) (Chen et al 1998;Han et al 2008;Liu et al 2010). Few relevant studies have been reported concerning the genome organization and chromosomal structure of wild species, except for some concerning chromosome morphology and karyotype (Chen et al 1999;Zhao et al 2011;Yagi et al 2014Yagi et al , 2015.…”

Section: Introductionmentioning

confidence: 99%

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Zhang

Yang

et al. 2016

Genome

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Ribosomal DNAs are useful cytogenetic markers for chromosome analysis. Studies investigating site numbers and distributions of rDNAs have provided important information for elucidating genome organization and chromosomal relationships of many species by fluorescence in situ hybridization. But relevant studies are scarce for species of the genus Cucumis, especially in wild species. In the present study, FISH was conducted to investigate the organization of 45S and 5S rDNA among 20 Cucumis accessions, including cultivars and wild accessions. Our results showed that the number of 45S rDNA sites varied from one to five pairs in different accessions, and most of these sites are located at the terminal regions of chromosomes. Interestingly, up to five pairs of 45S rDNA sites were observed in C. sativus var. sativus, the species which has the lowest chromosome number, i.e., 2n = 14. Only one pair of 5S rDNA sites was detected in all accessions, except for C. heptadactylus, C. sp, and C. spp that had two pairs of 5S rDNA sites. The distributions of 5S rDNA sites showed more variation than 45S rDNA sites. The phylogenetic analysis in this study showed that 45S and 5S rDNA have contrasting evolutionary patterns. We find that 5S rDNA has a polyploidizationrelated tendency towards the terminal location from an interstitial location but maintains a conserved site number, whereas the 45S rDNA showed a trend of increasing site number but a relatively conserved location.

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Section: Introductionmentioning

confidence: 99%

Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution inCucumis

Zhang

Yang

et al. 2016

Genome

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“…Cucumber is a diploid plant species (2n = 2x = 14) [26]. Since cucumber genes have one allele in each sister chromosome, the CRISPR/Cas9 system can induce four variants of mutations in alleles of the target gene (Figure 7).…”

Section: Crispr/cas9-mediated Genome Editing In Transgenic Cucumber R...mentioning

confidence: 99%

Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle in Cucumber (Cucumis sativus)?

Kiryushkin,

Ilina,

Kiikova

et al. 2024

IJMS

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The architecture of the root system is fundamental to plant productivity. The rate of root growth, the density of lateral roots, and the spatial structure of lateral and adventitious roots determine the developmental plasticity of the root system in response to changes in environmental conditions. One of the genes involved in the regulation of the slope angle of lateral roots is DEEPER ROOTING 1 (DRO1). Its orthologs and paralogs have been identified in rice, Arabidopsis, and several other species. However, nothing is known about the formation of the slope angle of lateral roots in species with the initiation of lateral root primordia within the parental root meristem. To address this knowledge gap, we identified orthologs and paralogs of the DRO1 gene in cucumber (Cucumis sativus) using a phylogenetic analysis of IGT protein family members. Differences in the transcriptional response of CsDRO1, CsDRO1-LIKE1 (CsDRO1L1), and CsDRO1-LIKE2 (CsDRO1L2) to exogenous auxin were analyzed. The results showed that only CsDRO1L1 is auxin-responsive. An analysis of promoter–reporter fusions demonstrated that the CsDRO1, CsDRO1L1, and CsDRO1L2 genes were expressed in the meristem in cell files of the central cylinder, endodermis, and cortex; the three genes displayed different expression patterns in cucumber roots with only partial overlap. A knockout of individual CsDRO1, CsDRO1L1, and CsDRO1L2 genes was performed via CRISPR/Cas9 gene editing. Our study suggests that the knockout of individual genes does not affect the slope angle formation during lateral root primordia development in the cucumber parental root.

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