V-Type ATPase Mediates Airway Surface Liquid Acidification in Cultured Pig Small Airway Epithelial Cells

Li, X.; Thornell, Ian M.; Rada, R. Villacreses; Brommel, Christian M; Lu, Lijun; Mather, Steven E; Ehler, Annie; Karp, Philip H.; Welsh, Michael J.; Zabner, Joseph

doi:10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a2573

Cited by 2 publications

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“…The contribution of V-ATPase to ASL pH remains unclear. Whereas some groups observed that bafilomycin, a specific V-ATPase blocker, raises ASL pH in Calu3 cells and in distal pig bronchi [ 56 , 62 , 189 ], others found that bafilomycin has minimal or no effects in human, pig, and cow airway epithelia [ 70 , 171 , 182 , 190 ]. Of note, a study on primary nasal epithelial cells and cultured lung showed that Pseudomonas aeruginosa inactivates V-ATPase, thereby reducing the expression and trafficking of CFTR [ 191 ].…”

Section: Acid Transport In Airway Cellsmentioning

confidence: 99%

Airway Surface Liquid pH Regulation in Airway Epithelium Current Understandings and Gaps in Knowledge

Zając

Dreano

Edwards

et al. 2021

IJMS

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Knowledge on the mechanisms of acid and base secretion in airways has progressed recently. The aim of this review is to summarize the known mechanisms of airway surface liquid (ASL) pH regulation and their implication in lung diseases. Normal ASL is slightly acidic relative to the interstitium, and defects in ASL pH regulation are associated with various respiratory diseases, such as cystic fibrosis. Basolateral bicarbonate (HCO3−) entry occurs via the electrogenic, coupled transport of sodium (Na+) and HCO3−, and, together with carbonic anhydrase enzymatic activity, provides HCO3− for apical secretion. The latter mainly involves CFTR, the apical chloride/bicarbonate exchanger pendrin and paracellular transport. Proton (H+) secretion into ASL is crucial to maintain its relative acidity compared to the blood. This is enabled by H+ apical secretion, mainly involving H+/K+ ATPase and vacuolar H+-ATPase that carry H+ against the electrochemical potential gradient. Paracellular HCO3− transport, the direction of which depends on the ASL pH value, acts as an ASL protective buffering mechanism. How the transepithelial transport of H+ and HCO3− is coordinated to tightly regulate ASL pH remains poorly understood, and should be the focus of new studies.

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Section: Acid Transport In Airway Cellsmentioning

confidence: 99%

Airway Surface Liquid pH Regulation in Airway Epithelium Current Understandings and Gaps in Knowledge

Zając

Dreano

Edwards

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…al. describe how they tackled these hurdles using ex vivo pig small airway explants and in vitro pig small airway cell cultures [12]. Their studies reveal a small airway-specific mechanism regulating ASL pH via a vacuolar type H + ATPase, ATP6V0D2 (Figure 1).…”

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confidence: 99%