V.—the Determination of Calcium in Histological Sections

Stock, Alexander

doi:10.1111/j.1365-2818.1949.tb00957.x

Cited by 17 publications

(8 citation statements)

References 2 publications

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“…18) of the posterior end of the abdomen were treated with the following methods. Staining with toluidine blue for the detection of mucous cells (Sylven, 1941); staining with purpurin (Lison, 1936) and gallamine blue (Stock, 1949) blue. Other sections stained with toluidine blue and compared with alternate sections, which are incinerated, show that the ventral epithelial mucous cells contain a large amount of calcium.…”

Section: The Posterior Calcareous Gratingmentioning

confidence: 99%

Tube Formation by Pomatoceros Triqueter (Polychaeta)

Hedley

1958

J. Mar. Biol. Ass.

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Section: The Posterior Calcareous Gratingmentioning

confidence: 99%

Tube Formation by Pomatoceros Triqueter (Polychaeta)

Hedley

1958

J. Mar. Biol. Ass.

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“…Calcium salts are stored in the digestive gland, and tissue treated for the identification of calcium deposits by von Kossa's (Glick, 1949) method, or the galamine blue process (Stock, 1949), showed that it is widely distributed in the basal part of the cytoplasm of all the digestive cells, not localised in certain lime cells. The tubules of the digestive gland are united by a thin layer of connective tissue and muscular cells and blood vessels penetrate through the connective tissue.…”

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confidence: 99%

ALIMENTARY AND REPRODUCTIVE SYSTEMS OF OXYCHILUS CELLARIUS (MÜLLER) (STYLOMMATOPHORA)

Rigby

1963

Proceedings of the Zoological Society of London

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Detailed study of the life cycle and the anatomy, histology and functioning of the alimentary and reproductive systems of Oxychilua cellariua (Müller) lead one to regard this snail as a typical and relatively unspecialized member of the Stylommatophora. Feeding currents within the oesophagus and staomach have been examined during dissection of fresh specimens and the form of the stomach region has been homologized with that of more primitive gastropods. Histochemical methods have been used to determine the nature of cell contents and secretions along the reproductive tract and the possible part played by these substances during copulation and egg‐laying has been investigated. The path used by sperm prior to, and following, copulation has been traced. Comparison is made with members of the higher Basommatophora, the Hygrophila. Much greater structural and functional differences become apparent between members of the Stylommatophora and the Basommatophora than have hitherto been suspected.

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“…Some evidence that calcium does not react under the conditions of test comes from the distribution of calcium in insect tissues which, when determined by buffered Gallamine blue (Stock 1949), was found to be far more widespread than that of rhodizonate staining. Tissues that stain with rhodizonate frequently also stain with Gallamine blue, but there are many tissues that stain intensely with Gallamine blue but do not react with rhodizonate (e.g.…”

Section: (C) Di8tribution Of Barium and Strontium In Insects On Metalmentioning

confidence: 99%

“…When granules, smeared on a slide, were treated with Gallamine blue, which forms a specific purple lake with calcium (Stock 1949), all or almost all of the granules became stained, indicating the presence of calcium. This suggests that calcium may be an interfering metal.…”

Section: }'{Eswlts ( A) Specificity Of the Rhodizonate Reactionmentioning

confidence: 99%

The Occurrence of Barium and Strontium in Insects

Waterhouse¹

1951

Aust. Jnl. Of Bio. Sci.

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SummaryBarium and strontium may be detected histochemically by the formation of reddish compounds following the treatment of tissues with sodium rhodizonate. Calcium does not appear to react under the neutral conditions employed in the test. Treatment with chromate inhibits the reaction with barium, but not with strontium, providing a means for distinguishing between these elements. Permanent histological sections can bc produced in the usual manner, so long as a neutral fixative is employed.Both barium and strontium were detected in the tissues of many insects. They occurred most frequently in the malpighian tubules, less often in the midgut and reproductive organs, and very occasionally in the hindgut and fat body. It is probable that both elements are absorbed in the midgut of most insects. The distribution of barium and strontium was characteristic for each species. When staining occurred it was almost always of granules, which frequently had a characteristically restricted distribution within the cell. Nucleoli stained occasionally, although the remainder of the nucleus seldom reacted.The possible importance of barium and strontium in insect metabolism is discussed. However, the granules detected are regarded as being either reserves for future use or as a form of storage excretion.

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V.—the Determination of Calcium in Histological Sections

Cited by 17 publications

References 2 publications

Tube Formation by Pomatoceros Triqueter (Polychaeta)

Tube Formation by Pomatoceros Triqueter (Polychaeta)

ALIMENTARY AND REPRODUCTIVE SYSTEMS OF OXYCHILUS CELLARIUS (MÜLLER) (STYLOMMATOPHORA)

The Occurrence of Barium and Strontium in Insects

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