V, 2.Ribosomal frameshifting in astroviruses

Brierley, Ian; Vidakovic, Marijana

doi:10.1016/s0168-7069(03)09035-9

Cited by 3 publications

(2 citation statements)

References 73 publications

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“…Furthermore, recent studies comparing multiple human and animal astroviruses have identified a functional ORF, encoding a viroporin, and previously suggested by in silico analyses [ 17 , 37 , 38 ]. These studies and other studies demonstrate the usefulness of in silico analysis [ 39 , 40 ].…”

Section: Introductionsupporting

confidence: 71%

Comparative Analysis of Novel Strains of Porcine Astrovirus Type 3 in the USA

Ferreyra

Harmon

Bradner

et al. 2021

Viruses

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Porcine astrovirus type 3 (PoAstV3) has been previously identified as a cause of polioencephalomyelitis in swine and continues to cause disease in the US swine industry. Herein, we describe the characterization of both untranslated regions, frameshifting signal, putative genome-linked virus protein (VPg) and conserved antigenic epitopes of several novel PoAstV3 genomes. Twenty complete coding sequences (CDS) were obtained from 32 diagnostic cases originating from 11 individual farms/systems sharing a nucleotide (amino acid) percent identity of 89.74–100% (94.79–100%), 91.9–100% (96.3–100%) and 90.71–100% (93.51–100%) for ORF1a, ORF1ab and ORF2, respectively. Our results indicate that the 5′UTR of PoAstV3 is highly conserved highlighting the importance of this region in translation initiation while their 3′UTR is moderately conserved among strains, presenting alternative configurations including multiple putative protein binding sites and pseudoknots. Moreover, two predicted conserved antigenic epitopes were identified matching the 3′ termini of VP27 of PoAstV3 USA strains. These epitopes may aid in the design and development of vaccine components and diagnostic assays useful to control outbreaks of PoAstV3-associated CNS disease. In conclusion, this is the first analysis predicting the structure of important regulatory motifs of neurotropic mamastroviruses, which differ from those previously described in human astroviruses.

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Section: Introductionsupporting

confidence: 71%

Comparative Analysis of Novel Strains of Porcine Astrovirus Type 3 in the USA

Ferreyra

Harmon

Bradner

et al. 2021

Viruses

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“…First, the ORF1a stop codon is located after the highly conserved heptamer signal and before the stem-loop structure, a typical characteristic of avian astroviruses (Fig. 4 A) [ 43 , 44 ]. Ribosomal frameshifting is induced by the above two cis-acting elements [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of astroviruses detected in wild aquatic birds in Hong Kong

Ng,

Sun,

Sit

et al. 2024

Virol J

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Wild waterfowl serve as a reservoir of some astroviruses. Fecal samples from wild waterfowl collected at Hong Kong's Marshes were tested using pan-astrovirus reverse transcription-PCR. Positive samples underwent subsequent host identification using DNA barcoding. Based on deduced partial sequences, noteworthy samples from three astrovirus groups (mammalian, avian and unclassified astroviruses) were further analyzed by next-generation sequencing. One sample of Avastrovirus 4 clade, MP22-196, had a nearly complete genome identified. The results of ORF2 phylogenetic analysis and genetic distance analysis indicate that Avastrovirus 4 is classified as a distinct subclade within Avastrovirus. MP22-196 has typical astrovirus genome characteristics. The unique characteristics and potential differences of this genome, compared to other avian astrovirus sequences, involve the identification of a modified sgRNA sequence situated near the ORF2 start codon, which precedes the ORF1b stop codon. Additionally, the 3' UTR of MP22-196 is shorter than other avian astroviruses. This study expands our understanding of the Avastrovirus 4 clade.

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