UVH6, a Plant Homolog of the Human/Yeast TFIIH Transcription Factor Subunit XPD/RAD3, Regulates Cold-stress Genes in Arabidopsis thaliana

Hall, Jennifer; Cobb, Jason; Iqbal, Maaz; Abidali, Moustapha; Liu, Zongrang; Mount, David W.

doi:10.1007/s11105-008-0076-x

Cited by 4 publications

(4 citation statements)

References 56 publications

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“…As a component of TFIIH, XPD/UVH6 plays an important role in transcription. uvh6 mutants have been reported to exhibit aberrant levels of several RNAs and proteins ( Jenkins et al 1997 ; Liu et al 2008 ; Hall et al 2009 ). With respect to its role in heat tolerance, uvh6 was reported to contain increased levels of HSP21 ( Jenkins et al 1997 ) but normal levels of HSP101 and sHSPs ( Larkindale et al 2005 ).…”

Section: Resultsmentioning

confidence: 99%

“…For example, Liu et al (2008) show that heat treatment reduced levels of AtKu70 and AtKu80 transcript in wild type, but this down-regulation did not occur in uvh6 . In response to another stress, cold, Hall et al (2009) found that uvh6 mutants failed to induce some cold stress genes but not all. Thus the uvh6-1 mutant appears to exhibit abnormal regulation of a subset of genes rather than global defects in transcription ( Hall et al 2009 ).…”

Section: Discussionmentioning

confidence: 99%

“…In response to another stress, cold, Hall et al (2009) found that uvh6 mutants failed to induce some cold stress genes but not all. Thus the uvh6-1 mutant appears to exhibit abnormal regulation of a subset of genes rather than global defects in transcription ( Hall et al 2009 ). In det1 mutants, we also detect abnormal levels of HSP transcripts, consistent with previous studies implicating DET1 in regulation of gene expression ( Benvenuto et al 2002 ; Schroeder et al 2002 ; Ma et al 2003 ; Lau et al 2011 ).…”

Section: Discussionmentioning

confidence: 99%

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Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Kim

Hatherell

et al. 2012

G3 Genes|Genomes|Genetics

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Plants must adapt to a variety of abiotic inputs, including visible light, ultraviolet (UV) light, and heat. In Arabidopsis thaliana, DE-ETIOLATED 1 (DET1) plays a role in visible light signaling, UV tolerance, and development. UV-HYPERSENSITIVE 6 (UVH6) mutants are UV and heat sensitive, as well as dwarf and pale, like det1. In this study, we examine the genetic interactions between these two genes. In dark-grown seedlings, uvh6 exhibits a weak de-etiolated phenotype but does not affect the stronger de-etiolated phenotype of det1. In the light, det1 is epistatic to uvh6 with regard to chlorophyll level, but their effect on all size parameters is additive and therefore independent. With regard to UV tolerance, det1 UV resistance is epistatic to uvh6 UV sensitivity. In heat stress experiments, det1 enhances heat-induced tissue damage in the uvh6 background but suppresses heat-induced growth inhibition. Thus, det1 acts epistatically to uvh6 with respect to de-etiolation, chlorophyll level, UV tolerance, and heat-induced growth inhibition, whereas det1 and uvh6 act additively to regulate plant size and heat-induced cell death. These data provide insight into interplay between light and heat signaling.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Kim

Hatherell

et al. 2012

G3 Genes|Genomes|Genetics

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“…Hall et al . () found that uvh6 ‐ 1 mutants are sensitive to cold stress, and abnormally regulate a subset of cold stress‐responsive genes during transcription. Thus, UVH6 in Arabidopsis may function as a transcriptional regulator that induces the gene expression required to facilitate HS tolerance.…”

Section: Discussionmentioning

confidence: 99%

Histone acetyltransferase GCN5 is essential for heat stress‐responsive gene activation and thermotolerance in Arabidopsis

et al. 2015

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SUMMARYExposure to temperatures exceeding the normal optimum levels, or heat stress (HS), constitutes an environmental disruption for plants, resulting in severe growth and development retardation. Here we show that loss of function of the Arabidopsis histone acetyltransferase GCN5 results in serious defects in terms of thermotolerance, and considerably impairs the transcriptional activation of HS-responsive genes. Notably, expression of several key regulators such as the HS transcription factors HSFA2 and HSFA3, Multiprotein Bridging Factor 1c (MBF1c) and UV-HYPERSENSITIVE 6 (UVH6) is down-regulated in the gcn5 mutant under HS compared with the wild-type. Chromatin immunoprecipitation (ChIP) assays indicated that GCN5 protein is enriched at the promoter regions of HSFA3 and UVH6 genes, but not in HSFA2 and MBF1c, and that GCN5 facilitates H3K9 and H3K14 acetylation, which are associated with HSFA3 and UVH6 activation under HS. Moreover, constitutive expression of UVH6 in the gcn5 mutant partially restores heat tolerance. Taken together, our data indicate that GCN5 plays a key role in the preservation of thermotolerance via versatile regulation in Arabidopsis. In addition, expression of the wheat TaGCN5 gene re-establishes heat tolerance in Arabidopsis gcn5 mutant plants, suggesting that GCN5-mediated thermotolerance may be conserved between Arabidopsis and wheat.

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Genome stability in the uvh6 mutant of Arabidopsis thaliana

et al. 2014

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Plant XPD homolog UVH6 is the protein involved in the repair of strand breaks, and the excision repair and uvh6 mutant is not impaired in transgenerational increase in HRF. While analyzing the transgenerational response to stress in plants, we found that the promoter and gene body of Arabidopsis thaliana (Arabidopsis) XPD homolog UVH6 underwent hypomethylation and showed an increase in the level of transcript. Here, we analyzed the mutant of this gene, uvh6-1, by crossing it to two different reporter lines: one which allows for analysis of homologous recombination frequency (HRF) and another which makes it possible to analyze the frequency of point mutations. We observed that uvh6-1 plants exhibited lower rate of spontaneous homologous recombination but higher frequencies of spontaneous point mutations. The analysis of strand breaks using ROPS and Comet assays showed that the mutant had a much higher level of strand breaks at non-induced conditions. Exposure to stresses such as UVC, heat, cold, flood and drought showed that the mutant was not impaired in an increase in somatic HRF. The analysis of spontaneous HRF in the progeny of control plants compared to that of the progeny of stressed plants demonstrated that uvh6-1 was mildly affected in response to temperature, UV and drought. Our data suggest that UVH6 may be involved in the repair of strand breaks and excision repair, but it is unlikely that UVH6 is required for transgenerational increase in HRF.

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UVH6, a Plant Homolog of the Human/Yeast TFIIH Transcription Factor Subunit XPD/RAD3, Regulates Cold-stress Genes in Arabidopsis thaliana

Cited by 4 publications

References 56 publications

Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Histone acetyltransferase GCN5 is essential for heat stress‐responsive gene activation and thermotolerance in Arabidopsis

Genome stability in the uvh6 mutant of Arabidopsis thaliana

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