UVA/UVB Irradiation Exerts a Distinct Phototoxic Effect on Human Keratinocytes Compared to Human Malignant Melanoma Cells

Gag, Otilia; Dinu, Ștefania; Manea, Horatiu Cristian; Marcovici, Iasmina; Pînzaru, Iulia; Popovici, Ramona Amina; Crăiniceanu, Zorin; Gyori, Zsolt; Iovănescu, Gheorghe; Chiriac, Sorin Dan

doi:10.3390/life13051144

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…Fortunately, UVB travels only to the stratum corneum of the epidermal layer. Moreover, energy loss of UVB occurs during its passage through the atmosphere, resulting in an energy level that is 100-fold less than that of UVA at sea level. , Therefore, the phototoxic potential of a test chemical against UVB is considered to be less important than that against UVA for systemic formulations. However, UVB-induced phototoxicity is related to topical formulations, especially UVB filter substances…”

Section: Resultsmentioning

confidence: 99%

BSA Adsorption on Titanium Dioxide Nanoparticle Surfaces for Controlling Their Cellular Uptake in Skin Cells

Thiramanas,

Wongngam,

Supanakorn

et al. 2024

ACS Appl. Bio Mater.

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Nanoparticles (NPs) are continuously being developed for many applications including imaging, biomedicine, and everyday products. It is difficult to avoid contact with NPs such as titanium dioxide (TiO 2 ) NPs, which are widely used in sunscreens. However, the safety of TiO 2 NPs for skin contact and inhalation remains controversial. If NPs cannot penetrate the skin, they will be unable to circulate in the bloodstream, accumulate in the body, or cause side effects, ensuring their safety. Therefore, this study aimed to modify TiO 2 NP surfaces to inhibit their uptake in skin cells. Inspired by protein corona studies, bovine serum albumin (BSA) was chosen to functionalize TiO 2 NP surfaces via physical adsorption. The maximum BSA adsorption occurred at pH 5.0. The physicochemical properties (size, ζ-potential, morphology, ultraviolet (UV) absorption efficiency, and sun protection factor (SPF)) of TiO 2 −BSA NPs were comparable to those of TiO 2 NPs, indicating that these properties did not affect cellular uptake. In the safety evaluation, TiO 2 NPs and TiO 2 −BSA NPs exhibited high biocompatibility with skin cells and no phototoxicity after UVA and UVB irradiation. In the efficacy evaluation, both NPs possessed the same photoprotection abilities, reducing membrane damage and DNA breakage after UVA irradiation. Compared with TiO 2 NPs, TiO 2 −BSA NPs showed substantially reduced skin penetration in Franz diffusion cells (91%) and human immortalized keratinocyte (HaCaT) cells (89%). A qualitative cellular uptake study using transmission electron microscopy and confocal laser scanning microscopy confirmed that TiO 2 NPs were more abundant than TiO 2 −BSA NPs inside the HaCaT cells. These findings indicate that TiO 2 surface functionalization with BSA inhibits cellular uptake in skin cells while maintaining safety and UV protection efficacy, which might be extended to other NP-based sunscreens.

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Section: Resultsmentioning

confidence: 99%

BSA Adsorption on Titanium Dioxide Nanoparticle Surfaces for Controlling Their Cellular Uptake in Skin Cells

Thiramanas,

Wongngam,

Supanakorn

et al. 2024

ACS Appl. Bio Mater.

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Recycled Cellulose Acetate/Cerium Vanadate Nanoparticles Composite Membranes with Tuned Ultraviolet and Blue Light Shielding Capabilities

Yousef,

Taha,

Ali

et al. 2024

ACS Appl. Polym. Mater.

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Ultraviolet radiation poses health risks with both immediate and long-term consequences. It is linked to various skin conditions such as skin cancer, sunburn, and aging. Radiation can negatively impact medication efficacy. UV radiation alters the color, texture, and taste of food, potentially making it unsuitable for consumption. Therefore, UV-shielding materials see significant research focus for various applications. Herein, different concentrations of CeVO 4 nanoparticles were embedded in recycled cellulose acetate (CA) matrix to fabricate UV-shielding nanocomposite membranes. The UVC, UVB, UVA, and blue light transmittance through those membranes has been investigated. The addition of CeVO 4 nanoparticles to the CA membrane resulted in a slight decrease in contact angle, indicating increased surface roughness and effective encapsulation of the nanoparticles within the CA matrix. The contact angle ranging from 64.22°to 65°indicates moderate hydrophilicity and a tendency for hydrophobicity, facilitating a stable and uniform distribution of the nanoparticles within the CA matrix. This balanced distribution is crucial to ensure sustained effectiveness in shielding against UV rays across the entire membrane. The recycled CA membrane and membranes containing 0.2 and 0.3 wt % CeVO 4 exhibited similar UVC blocking behavior. The blocking efficiency of UVB remained stable for all CeVO 4 -containing membranes except for the membrane containing 0.6 wt % CeVO 4 , which exhibited a slightly increased transmittance after irradiation. Moreover, increasing the concentration of CeVO 4 in the CA matrix resulted in enhanced blue light blocking. Therefore, the 0.1 wt % CeVO 4 @CA membrane is recommended for shielding against UVC and UVB, while the 0.6 wt % CeVO 4 @CA membrane is suitable for UVA and blue light blocking. This study introduces an approach that utilizes recycled CA and flowershaped CeVO 4 nanoparticles to fabricate membranes with enhanced thermal stability, superior light-blocking capabilities, and excellent optical properties. The results provide valuable insights into the development of advanced light-blocking membranes, addressing the limitations observed in previous studies and opening up possibilities for various applications requiring effective light shielding.

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UVA/UVB Irradiation Exerts a Distinct Phototoxic Effect on Human Keratinocytes Compared to Human Malignant Melanoma Cells

Cited by 2 publications

References 47 publications

BSA Adsorption on Titanium Dioxide Nanoparticle Surfaces for Controlling Their Cellular Uptake in Skin Cells

BSA Adsorption on Titanium Dioxide Nanoparticle Surfaces for Controlling Their Cellular Uptake in Skin Cells

Recycled Cellulose Acetate/Cerium Vanadate Nanoparticles Composite Membranes with Tuned Ultraviolet and Blue Light Shielding Capabilities

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