UVA-induced antimicrobial activity of ZnO/Ag nanocomposite covered surfaces

Visnapuu, Meeri; Rosenberg, Merilin; Truska, Egle; Nõmmiste, E.; Šutka, Andris; Kahru, Anne; Rähn, Mihkel; Vija, Heiki; Orupõld, Kaja; Kisand, Vambola; Ivask, Angela

doi:10.1016/j.colsurfb.2018.05.009

Cited by 38 publications

(39 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ag from the nano-ZnO/Ag surfaces decreased planktonic viability of only E. coli, but not S. aureus in oligotrophic conditions ( Fig. 2A, 2B) which is in concordance with our previous findings that S. aureus is more sensitive to Zn and E. coli more sensitive to Ag [20]. Maximum calculated Ag concentration in the test system in case of complete release of Ag from the surface coating was estimated to reach 15.27±2.21 ng/ml ( Supplementary Table 1).…”

Section: Bacterial Biofilm Formation On Surfaces Coated With Nano-znosupporting

confidence: 93%

“…To assess anti-adhesion and metal release-associated antimicrobial properties of the coating materials, biofilm formation on the sparsely and densely coated nano-ZnO and sparsely coated nano-ZnO/Ag surfaces was studied. Biofilms were either grown in static oligotrophic environment (1:500 diluted nutrient broth in synthetic tap water, not supporting growth) at room temperature to mimic real-life-like use in moist environments (similar to previously used standard conditions for planktonic testing [20]) or in nutrient-rich growth mediums (LB, YPD; supporting exponential growth) resembling classical laboratory approach for studying biofilms [21]. E. coli MG1655 was used as a Gram-negative, S. aureus ATCC25923 as a Gram-positive and C. albicans CA14 as a human-relevant fungal model organism.…”

Section: Resultsmentioning

confidence: 99%

“…Nano-ZnO/Ag composite particles were used only in sparse coating density. Physical and chemical properties of the nanoparticles used as well photocatalytic and antibacterial activity of the surfaces used in this study have been previously described [20]. Total Zn content in the nano-coatings was analyzed using TXRF (S2 Picofox, Bruker) and Ag content with AAS (ContrAA 800, Analytic Jena AG) after 1 h treatment in 0.5 ml concentrated HNO3.…”

Section: Surfacesmentioning

confidence: 99%

“…We have previously reported developing novel multi-effective antimicrobial coatings based on nano-ZnO/Ag composite particles [20]. The novelty of these coatings rises from a combined and complex effect of different antimicrobial mechanisms: (i) antimicrobial activity of Zn 2+ ions, (ii) antimicrobial activity of Ag + ions, and (iii) antimicrobial activity of ROS, generated at the surface of nano-ZnO and nano-ZnO/Ag via photocatalytic processes under UV-A illumination.…”

Section: Introductionmentioning

confidence: 99%

“…Photodepositing Ag onto the nano-ZnO increased its photocatalytic activity and acted as an additional antimicrobial agent in the absence of UV-A exposure. [20] With the intent to further develop these materials for use on high-touch surfaces in the public spaces, in this study we additionally assessed the efficacy of the surface materials against biofilm formation in application-appropriate oligotrophic environment and in the absence of UV-A exposure. For that we opted for a static model to grow biofilms directly on the studied surfaces and to be able to monitor dissolutiondriven toxicity to the planktonic cells in a small closed system.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Rosenberg

Visnapuu

Vija

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Spread of pathogenic microbes and antibiotic-resistant bacteria in health-care settings and public spaces is a serious public health challenge. Materials and surface-treatments that prevent solid surface colonization and biofilm formation or impede touch-transfer of viable microbes could provide means to decrease pathogen transfer from high-touch surfaces in critical applications. Both, ZnO and Ag nanoparticles have shown a great potential in antimicrobial applications. Although antimicrobial properties of such nanoparticle suspensions and their biocompatibility with human cells are well studied, less is known about nano-enabled solid surfaces. Here we demonstrate that surfaces coated with nano-ZnO or nano-ZnO/Ag composites possess species-selective medium-dependent antibiofilm activity against Escherichia coli, Staphylococcus aureus and Candida albicans. Colonization of nano-ZnO surfaces by E. coli and S. aureus was decreased in oligotrophic (nutrient-poor, no growth) conditions with E. coli showinghigher sensitivity to Ag and S. aureus to Zn, respectively. Minor to no effect was observed for bacteria in growth medium (nutrient-rich, exponential growth). Inversely, compared to uncoated surfaces, nano-ZnO surfaces enhanced biofilm formation by C. albicans in oligotrophic conditions and just a minor transient negative effect was seen in nutrient-rich medium. However, enhanced C. albicans biofilm formation on nano-ZnO surfaces was effectively counteracted by the addition of Ag. Our results not only showed that nano-ZnO/Ag coated solid surfaces have the potential to effectively decrease surface colonization by the bacteria E. coli and S. aureus but also indicated the importance of the use of application-appropriate test conditions and exposure medium in antimicrobial surface testing. Possible selective enhancement of biofilm formation by the yeast C. albicans on Zn-enabled surfaces should be taken into account in antimicrobial surface development.

show abstract

Section: Bacterial Biofilm Formation On Surfaces Coated With Nano-znosupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Surfacesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Rosenberg

Visnapuu

Vija

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Bacterial Lysis via Photocatalysis ‐ A Critical Mechanistic Review

Gupta

Modak

2020

ChemCatChem

View full text Add to dashboard Cite

This Review discusses one of the most important area of photocatalytic reactions. In current research scenario, photocatalysis is well utilized strategy for a wide variety of applications including organic/ inorganic decontamination, solar fuel generation, microbial disinfection etc. This Review touches upon the mechanistic aspect of microbial disinfection, the growth in the research, issues to be addressed in future to make it a sustainable technology for this application. This Review discusses the fundamental understanding of photocatalysis, important strategies to tune their optical properties to enhance the spectral range of absorption in visible and near infrared wavelength region. Various photocatalysis reports these days don't focus on the stability, reusability and recovery of the photocatalysts. However, these issues are very important to deal with for large scale viability of the process. Therefore, we have tried to address these issues along with the detailed experimental information and eliminate the misconceptions about bacterial disinfection. The details provided in this Review would help the researchers working in this area to a large extent and would open various questions for them to answer.

show abstract

Unraveling the mechanisms of inhibition of silver‐doped bioactiveglass–ceramicparticles

Pajares-Chamorro

Lensmire

Hammer

et al. 2022

J Biomedical Materials Res

View full text Add to dashboard Cite

Infections are a major concern in orthopedics. Antibacterial agents such as silver ions are of great interest as broad‐spectrum biocides and have been incorporated into bioactive glass–ceramic particles to control the release of ions within a therapeutic concentration and provide tissue regenerative properties. In this work, the antibacterial capabilities of silver‐doped bioactive glass (Ag‐BG) microparticles were explored to reveal the unedited mechanisms of inhibition against methicillin‐resistant Staphylococcus aureus (MRSA). The antibacterial properties were not limited to the delivery of silver ions but rather a combination of antibacterial degradation by‐products. For example, nano‐sized debris punctured holes in bacteria membranes, osmotic effects, and reactive oxygen species causing oxidative stress and almost 40% of the inhibition. Upon successive Ag‐BG treatments, MRSA underwent phenotypic and genomic mutations which were not only insufficient to develop resistance but instead, the clones became more sensitive as the treatment was re‐delivered. Additionally, the unprecedented restorative functionality of Ag‐BG allowed the effective use of antibiotics that MRSA resists. The synergy mechanism was mainly identified for combinations targeting cell‐wall activity and their action was proven in biofilm‐like and virulent conditions. Unraveling these mechanisms may offer new insights into how to tailor healthcare materials to prevent or debilitate infections and join the fight against antibiotic resistance in clinical cases.

show abstract

UVA-induced antimicrobial activity of ZnO/Ag nanocomposite covered surfaces

Cited by 38 publications

References 44 publications

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Bacterial Lysis via Photocatalysis ‐ A Critical Mechanistic Review

Unraveling the mechanisms of inhibition of silver‐doped bioactiveglass–ceramicparticles

Contact Info

Product

Resources

About