UV-Visible Spectrophotometric as a Prospective Tool in Neonatal Sepsis

Suhartono, Eko; Yunanto, Ari; Hartoyo, Edi; Kania, Nia; Utama, Adelia Anggraini; Sari, Ratih Kumala; Putri, Niarsari Anugrahing; Thalib, Iskandar

doi:10.18585/inabj.v10i1.360

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…The HOSCN that produced in sepsis condition not only play role in killing the invading pathogen, but also promote a host tissue damage such as the protein oxidation. AOPP is already known as one of the oxidized protein and has been used as oxidative stress marker for several disease such as NS [23]. The AOPP level measurement in both case and control group shows in Figure 4.…”

Section: Resultsmentioning

confidence: 97%

Placental neutrophil count as an early predictor in neonatal sepsis

2018

J. adv. health med. sci.

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This present study aims to assess the Placenta Neutrophil Count (PNC) as an early marker in the diagnosis of Neonatal Sepsis (NS). This study was conducted in February-May 2017. Cord blood samples and placental tissues were taken from 15 each of newborns at risk of sepsis (case group) and without risk of sepsis (control group). The PNC was estimated using conventional light microscope. We also measured some oxidative stress parameters from the cord blood samples, i.e., Hydrogen Peroxide (H 2 O 2 ), thiocyanate (SCN), myeloperoxidase (MPO) enzyme and Advance Oxidation Protein Products (AOPPs) to investigate the role of neutrophil in placenta. The results show that the PNC, the level of AOPPs and SCN, and MPO activity of cord blood were higher in the case group than the control group, while for the H 2 O 2 level show the opposite result.From this results, it can be assumed thatneutrophil have a role in NS and might be use as a potential marker for early prediction of NS. These indings need to be veri ied by further investigation of fresh samples, in a larger, prospective cohort, and with sophisticated analysis evidenced by deep sequencing. The placental pathologic and histologic examination has been used as a diagnostic tool for neonatologist in many neonatal diseases, except NS. This result suggested that PNC might can be used as an early predictor for NS.

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Section: Resultsmentioning

confidence: 97%

Placental neutrophil count as an early predictor in neonatal sepsis

2018

J. adv. health med. sci.

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“…This might have relation to the occurrence of EONS, since premature and LBW are risk factors for neonatal sepsis. (5,30) Increasing the risk of infection later born to eventually become neonatal sepsis lack of immunoglobulin G (IgG) antibodies causes premature neonates to have an immature immune system. (31,32) The median value of presepsin levels in this study was 108.33 ng/L, with minimum presepsin level was 71.43 ng/L and maximum level was 1287.76 ng/L.…”

Section: Discussionmentioning

confidence: 99%

Presepsin Levels and Neutrophil-to-Lymphocyte Ratio are Positively Correlated with Procalcitonin Levels in Early Onset Neonatal Sepsis

et al. 2023

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BACKGROUND: Early-onset neonatal sepsis (EONS) is an acute infection and sepsis occurring within the first 24 hours of new-born life. An increase in procalcitonin levels, presepsin levels, and neutrophil-to-lymphocyte ratio (NLR) in EONS subjects have been reported. However, whether presepsin levels and NLR affect the procalcitonin levels in EONS patients who have received antibiotic therapy has not been certainly known. This study was conducted to determine the correlation between presepsin levels and NLR with procalcitonin levels in EONS subjects.METHODS: A cross-sectional study involving 52 EONS subjects were conducted, and blood samples from subjects were collected. Presepsin levels were examined by enzyme-linked immunosorbent assay (ELISA) method, NLR was calculated from the absolute number of neutrophils divided by the absolute number of lymphocytes, and procalcitonin levels were examined by chemiluminescent microparticle immunoassay (CMIA) method. RESULTS: Median of procalcitonin levels and presepsin levels were 0.435 (0.12-9.11) ng/mL and 108.33 (71.43-1287.76) ng/L, respectively. While NLR value was 1.68 (0.2-7.52). There was significant difference between procalcitonin and presepsin levels (p=0.000), and so does between procalcitonin levels and NLR (p=0.001). Based on the multivariate analysis, presepsin levels also affected the procalcitonin levels (p=0.000; 95% CI: 0.001-0.004).CONCLUSION: The results of the study showed that there was significant correlation between presepsin levels and NLR with procalcitonin levels in EONS patients, suggesting that presepsin levels, NLR, and procalcitonin levels are potential candidates for EONS biomarkers.KEYWORDS: EONS, NLR, presepsin, procalcitonin

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“…A blank solution is a mixture between 200 ml of phosphate buffer solution, 10 ml of potassium iodide, and 20 ml of acetic acid. AOPPs concentrations were expressed as mmol/l of chloramine-T equivalents [13].…”

Section: Aopps Concentration Analysismentioning

confidence: 99%

New Molecular Mechanism of Ceftazidime-Induced Human Red Blood Cell Hemolysis Through the Photohemolysis Reaction

Mashuri

Zaini

Rahmanisa

et al. 2018

Asian J Pharm Clin Res

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Objective: The present study was undertaken to investigate the photohemolysis reaction through photosensitization reaction by ceftazidime as a photosensitizer in human red blood cell (RBC).Methods: In this present study, human erythrocytes have used a sample. The sample then divided into six groups consisting of Group 1 (T1) served a negative control which consists of erythrocytes and buffers phosphate with pH 6.8; Group 2 (T2) served as a positive control which consists erythrocytes and buffers phosphate with pH 6.8 and exposed to UV-light; and Group 3, 4, 5, and 6 (T3, T4, T5, and T6) served as an experimental group which consists of erythrocytes, buffer phosphate with pH 6.8, ceftazidime with concentration 10%, 20%, 30%, and 40%, respectively, and also exposed to UV-light. UV-light exposure was done in 2 h. After the treatment period, the level of hydrogen peroxide (H2O2), conjugated diene (CD), advanced oxidation protein products (AOPPs), and percentage of RBC hemolysis (RBCH) were measured.Results: The results of this present studies showed that ceftazidime significantly increases the levels of H2O2, CD, AOPPs, and percentage of RBCH during the UV radiation.Conclusion: The present study demonstrated that ceftazidime acts as a photosensitizer and induced the photohemolysis reaction in human RBC. Furthermore, the hemolysis of RBC seems through the protein damage than lipid damage.

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UV-Visible Spectrophotometric as a Prospective Tool in Neonatal Sepsis

Cited by 6 publications

References 9 publications

Placental neutrophil count as an early predictor in neonatal sepsis

Placental neutrophil count as an early predictor in neonatal sepsis

Presepsin Levels and Neutrophil-to-Lymphocyte Ratio are Positively Correlated with Procalcitonin Levels in Early Onset Neonatal Sepsis

New Molecular Mechanism of Ceftazidime-Induced Human Red Blood Cell Hemolysis Through the Photohemolysis Reaction

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