UV/VIS spectroelectrochemistry with 3D printed electrodes

Vaněčková, Eva; Bouša, Milan; Vivaldi, Federico; Gál, Miroslav; Rathouský, Jiřı́; Kolivoška, Viliam; Sebechlebská, Táňa

doi:10.1016/j.jelechem.2019.113760

Cited by 38 publications

(11 citation statements)

References 48 publications

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“…Similarly, for UV/VIS flow chemistry applications, polystyrene slides were used by Michalec et al [25], whilst, Michalec and Tymecki [23] 3D printed cuvette inserts for flow chemistry applications using commercial plastic cuvettes to obtain good spectroscopic data. Vaněčková et al [22] utilised quartz cuvettes to enable optical detection in their 3D printed spectroelectrochemistry devices.…”

Section: Discussionmentioning

confidence: 99%

“…In the chemical and biological sciences there is already a significant number of examples of published 3D printed devices [1][2][3][4][5][6], including reactionware [7][8][9][10] and microfluidic devices [11][12][13], together with accounts of spectroscopic applications [14][15][16][17][18][19][20][21][22][23][24][25]. There are, however, several challenges that need to be addressed before 3D printed devices can be routinely constructed for spectrophotometric applications, particularly for FFF printers.…”

Section: Introductionmentioning

confidence: 99%

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3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes

Sirjani

Migas

Cragg

et al. 2020

Additive Manufacturing

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Transparent materials for fused filament fabrication printers are widely available and may be useful for constructing 3D printed devices with applications in UV/VIS spectroscopy. In this study, colourless polylactic acid, HD Glass and T-Glase were evaluated as construction materials for biochemical sensors, which contain immobilised enzymes, for analysis by UV/VIS spectrophotometry. Experiments were conducted on both the native 3D print and after coating with XTC-3D ® , a transparent epoxy resin used to improve optical transparency of 3D prints. A combination of HD Glass or T-Glase with XTC-3D ® , gave the highest transparency, probably due to the similar refractive indices of these materials. Individual enzymes were immobilised within the 3D prints by coupling the enzymes to tosyl-activated magnetic beads and attracted to the print surface by magnets embedded in the 3D print. A transparent 3D printed device was demonstrated using enzymatic assays of lactose and glucose. This device can be configured to contain up to three separate reaction chambers and features a separate colorimetric analysis chamber. Further studies showed that enzyme assays performed in these 3D printed devices are reproducible, accurate and of comparable sensitivity to the same assays performed in polystyrene cuvettes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes

Sirjani

Migas

Cragg

et al. 2020

Additive Manufacturing

View full text Add to dashboard Cite

show abstract

“…We summarized such recent hybrid systems here, Sebechlebska et al demonstrated a 3D printed hybrid integrated sensor that integrates electrochemistry and UV/Vis absorption spectroscopy, dubbed as a first ever report of UV/Vis absorption spectroelectrochemical apparatus, employing 3D printed optically transparent working electrodes [ 110 ]. PLA based 3D printed electrodes made from carbon nanotubes ( Figure 9 A) were utilized in this study due to their higher electrical conductivity compared to PLA doped with carbon black and graphene.…”

Section: Hybrid 3d Printed Sensorsmentioning

confidence: 99%

“…All components arrangement, working- counter—and reference electrode arrangement along with optical viewing window shown. Reproduced with permission from [ 110 ]. Copyright (2019) Elsevier.…”

Section: Figurementioning

confidence: 99%

3D-Printed Immunosensor Arrays for Cancer Diagnostics

Sharafeldin

Kadimisetty

Bhalerao

et al. 2020

Sensors

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Detecting cancer at an early stage of disease progression promises better treatment outcomes and longer lifespans for cancer survivors. Research has been directed towards the development of accessible and highly sensitive cancer diagnostic tools, many of which rely on protein biomarkers and biomarker panels which are overexpressed in body fluids and associated with different types of cancer. Protein biomarker detection for point-of-care (POC) use requires the development of sensitive, noninvasive liquid biopsy cancer diagnostics that overcome the limitations and low sensitivities associated with current dependence upon imaging and invasive biopsies. Among many endeavors to produce user-friendly, semi-automated, and sensitive protein biomarker sensors, 3D printing is rapidly becoming an important contemporary tool for achieving these goals. Supported by the widely available selection of affordable desktop 3D printers and diverse printing options, 3D printing is becoming a standard tool for developing low-cost immunosensors that can also be used to make final commercial products. In the last few years, 3D printing platforms have been used to produce complex sensor devices with high resolution, tailored towards researchers’ and clinicians’ needs and limited only by their imagination. Unlike traditional subtractive manufacturing, 3D printing, also known as additive manufacturing, has drastically reduced the time of sensor and sensor array development while offering excellent sensitivity at a fraction of the cost of conventional technologies such as photolithography. In this review, we offer a comprehensive description of 3D printing techniques commonly used to develop immunosensors, arrays, and microfluidic arrays. In addition, recent applications utilizing 3D printing in immunosensors integrated with different signal transduction strategies are described. These applications include electrochemical, chemiluminescent (CL), and electrochemiluminescent (ECL) 3D-printed immunosensors. Finally, we discuss current challenges and limitations associated with available 3D printing technology and future directions of this field.

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“…The developed MOF-based 3D-printed device presents distinct and significant advantages over existing MOF-based electrodes used for the ASV of Pb(II) [13][14][15][16][17]. The use of 3D-printing technology for the preparation of the device offers plenty of smart features including desktop-sized equipment, very low costs, production speed, strict control of the printing parameters, and ease of printing operation, while it generates negligible non-toxic waste [20][21][22][23][24][25][26][27][28][29][30][31][32]. The adopted process of mixing a small quantity of MOF with GP to produce the WE is by far simpler than drop-casting leading to stand-alone sensors, as the surface of the WE is renewed via a slight pressure on the syringe plunger.…”

Section: Reagents and Apparatusmentioning

confidence: 99%

Voltammetric Determination of Pb(II) by a Ca-MOF-Modified Carbon Paste Electrode Integrated in a 3D-Printed Device

Vlachou

Margariti

Papaefstathìou

et al. 2020

Sensors

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In this work, a voltammetric method based on a metal organic framework (Ca-MOF)-modified carbon paste electrode for lead determination was developed. The MOF-based electrode was packed in a new type of 3D-printed syringe-type integrated device, which was entirely fabricated by a dual extruder 3D printer. After optimization of the operational parameters, a limit of detection of 0.26 µg L−1 Pb(II) was achieved, which is lower than that of existing MOF-based lead sensors. The device was used for Pb(II) determination in fish feed and bottled water samples with high accuracy and reliability. The proposed sensor is suitable for on-site analyses and provides a low-cost integrated transducer for the ultrasensitive routine detection of lead in practical applications.

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UV/VIS spectroelectrochemistry with 3D printed electrodes

Cited by 38 publications

References 48 publications

3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes

3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes

3D-Printed Immunosensor Arrays for Cancer Diagnostics

Voltammetric Determination of Pb(II) by a Ca-MOF-Modified Carbon Paste Electrode Integrated in a 3D-Printed Device

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