Calcareous nannofossils for provenance analysis is a new-old topic for cultural heritage. Several studies already mentioned it for ceramic but less for paintings. Preparatory layers of the paintings are often made with chalk which is composed of microfossils. In order to extract the calcareous nannofossils assemblage from these layers, we need to disaggregate it. Actually, the method is to plunge the micro-samples into the water and heat it if the water alone does not work. The resulting disaggregation takes long and is not efficient in terms of quantitative results or time. In this work, we aimed to develop a disaggregation method that will extract the most nannofossils from the preparatory layer with the least destruction. As these samples are unique and we should make the most of them, we observe if gas chromatography-mass spectrometer analysis could be done on the disaggregated sample in order to determine the binders after the nannofossils retrieval. The method we are presenting is relatively easy to use, can be applied for most paintings and have a low impact on binders for their determination.