UV spectrophotometric simultaneous estimation of valdecoxib and paracetamol in combined tablet dosage form

Nagulwar, Vaishali; Dhurvey, Y. R.; Deshpande, Shilpa; Upadhye, Kanchan; Bakhle, Suparna; Wadetwar, Rita N.

doi:10.4103/0250-474x.29634

Cited by 7 publications

(3 citation statements)

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“…[19–23] Few UV, RP-HPLC, HPTLC, and LC/MS/MS methods have been reported for the simultaneous estimation of LAMI with nevirapine, stavudine, and zidovudine. [24–28] Also, for the estimation of EFV, UV, HPTLC, and isocratic HPLC methods in tablet formulation and HPLC method in human plasma have been reported. [25–33] Also, RP-HPLC method is reported for the simultaneous estimation of EFV with TDF and emtricitabine.…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC method for simultaneous estimation of tenofovir disoproxil fumarate, lamivudine, and efavirenz in combined tablet dosage form

Bhavsar¹,

Patel²,

Patel³

2012

Pharmaceutical Methods

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Background:A simple, precise, accurate, and rapid reverse phase-high performance liquid chromatography (RP-HPLC) method with UV-Visible detector has been developed and subsequently validated for the simultaneous determination of tenofovir disoproxil fumarate (TDF), lamivudine (LAMI), and efavirenz (EFV) in their combined tablet dosage form.Materials and Methods:The separation was based on the use of a Kromasil C18 analytical column (150 × 4.6 mm, i.d., 5 μm). The mobile phase consisted of a mixture of 70 volumes of methanol and 30 volumes of 10 mM phosphate buffer (pH 5.0). The separation was carried out at 40°C temperature with a flow rate of 1 ml/min.Results:Quantitation was achieved with UV detection at 254 nm, with linear calibration curves at concentration ranges of 1–6 μg/ml for TDF and LAMI and 2–12 μg/ml for EFV. The recoveries obtained were 99.46–101.36% for LAMI, 99.57–101.42% for TDF, and 99.96–100.87 for EFV.Conclusion:The method was validated according to International conference of harmonisation guidelines in terms of accuracy, precision, specificity, robustness, limits of detection and quantitation, and other aspects of analytical validation.

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Section: Introductionmentioning

confidence: 99%

RP-HPLC method for simultaneous estimation of tenofovir disoproxil fumarate, lamivudine, and efavirenz in combined tablet dosage form

Bhavsar¹,

Patel²,

Patel³

2012

Pharmaceutical Methods

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“…The objectives of this study were firstly to develop and then validate two analytical methods for the quantification of AZT containing solid dosage form. [3][4][5][6] 2. Experimental…”

Section: Introductionmentioning

confidence: 99%

A quantitative analysis of Zidovudine containing formulation by FT-IR and UV spectroscopy

2010

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In this study IR and UV spectroscopic procedures are describe for the quantitative determination of Zidovudine (AZT) from solid dosage form. For IR spectroscopic method (KBr disc technique) has been used and Ursodeoxycholic acid (UDCA) was used as an internal standard. The specific absorption bands at 2105 and 2931 cm À1 were chosen for AZT and UDCA respectively. In this method, beer's law was obeyed in the concentration range 0.8-2.0% w/w in KBr disc. The regression equation was found to be y ¼ 1.088x + 1.194 with correlation coefficient 0.9819, and the assay was found to be 99.8% with RSD of 2.157%. The UV spectroscopic method was also used in the quantitative determination of AZT in solid dosage form. 266 nm was chosen as l max . The regression equation was found to be y ¼ 0.0434x + 0.027, and the correlation coefficient 0.9989. The assay was found to be 99.81% with RSD of 0.273% by the UV spectroscopic method.

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“…ETO and PA combination is especially used for the arthritis and pain however with these combination minor side effects like nausea and headache are reported. High Performance Thin Layer Chromatographic 9-11 , spectrophotometric [12][13][14] methods for determination of PA either in single or in combination with other drugs was reported. Analytical methods have been reported for the determination of ETO includes Spectrophotometric [15][16][17] and HPLC [18][19] as single component or in combinations with other drugs.…”

mentioning

confidence: 99%

Untitled

2017

IJPSR

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ABSTRACT:In current research work two methods have been developed for simultaneous determination of etodolac and paracetamol in binary mixtures. HPTLC-densitometry was employed for the determination of the mixture for etodolac 50-400 ng band⁻¹ and for paracetamol 50-300 ng band⁻¹. Separation was carried out on a silica gel 60 F 254 HPTLC plates, using toluene: acetone: methanol: glacial acetic acid (6:2:1:0.5 v/v) as mobile phase. Etodolac, paracetamol and the toxic impurity para-aminophenol were well resolved with R f values of 0.61±0.04, 0.41±0.04 and 0.20±0.03. Determination has been carried out at 254 nm with a mean percentage recovery of 99.77±1.30 for etodolac and 99.86 ± 0.97 for paracetamol. First derivative (D1) spectrophotometry was employed for simultaneous determination of etodolac (217nm) and paracetamol (236 nm). Linearity ranges of both the compounds were found to be 2.5-12.5µg mL⁻¹ with a mean percentage recovery of 98.07±1.62 for etodolac and100.65±0.84 for paracetamol respectively. Methods were validated according to ICH guidelines and successfully implemented for the analysis of bulk powder and pharmaceutical formulations.

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UV spectrophotometric simultaneous estimation of valdecoxib and paracetamol in combined tablet dosage form

Cited by 7 publications

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RP-HPLC method for simultaneous estimation of tenofovir disoproxil fumarate, lamivudine, and efavirenz in combined tablet dosage form

RP-HPLC method for simultaneous estimation of tenofovir disoproxil fumarate, lamivudine, and efavirenz in combined tablet dosage form

A quantitative analysis of Zidovudine containing formulation by FT-IR and UV spectroscopy

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