Abstract:Simple, rapid, sensitive, precise and specific UV Spectrophotometric for the determination of cetilistat in API and pharmaceutical dosage form were developed and validated. In this method solutions of cetilistat were prepared in n-hexane. Cetilistat standard solution was scanned in the UV range (400-200nm) in a 1cm quartz cell in a double beam UV spectrophotometer. The standard solution of cetilistat showed maximum absorption at wavelength 320.0 nm. The method obeys Beer's law in the concentration range from 2… Show more
“…One spectrophotometric (Kshirsagar et al, 2018) anda few liquid chromatographic methods (El-Shanawani, 1998;Pajchel & Tyski, 2002;Laviana, Fernandez, Bayod & Blanco, 2003;Ganesh et al, 2018) have been reported previously for the quantitative determination of sultamicillin in pharmaceutical dosage form. In the method of Kshirsagar et al 2018 absorbance measurements were made at 232 nm which is the absorption maximum of sultamicillin in methanol. For the assay of sultamicillin in pharmaceutical dosage forms, more selective analytical methods are required to avoid possible interferences of the excipients.…”
Background and Aims: A new sensitive and selective method for the determination of sultamicillin in bulk and pharmaceutical preparations has been developed. Methods: The developed method is based on the derivatization of sultamicillin with 4-chloro-7-nitrobenzofurazan in pH 9.0 borate buffer at 70ºC for 60 min. The derivative was measured spectrophotometrically at 432 nm after extraction with chloroform. Results: The method was validated by linearity, LOD, LOQ, accuracy, precision and recovery parameters. The linear range for sultamicillin was 10-50 µg.mL-1 with a correlation coefficient of 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.47 and 4.41 µg/mL-1 , respectively. Conclusion: The suggested method was validated, and can be applied for routine sultamicillin analysis in dosage formsfor industrial analysis.
“…One spectrophotometric (Kshirsagar et al, 2018) anda few liquid chromatographic methods (El-Shanawani, 1998;Pajchel & Tyski, 2002;Laviana, Fernandez, Bayod & Blanco, 2003;Ganesh et al, 2018) have been reported previously for the quantitative determination of sultamicillin in pharmaceutical dosage form. In the method of Kshirsagar et al 2018 absorbance measurements were made at 232 nm which is the absorption maximum of sultamicillin in methanol. For the assay of sultamicillin in pharmaceutical dosage forms, more selective analytical methods are required to avoid possible interferences of the excipients.…”
Background and Aims: A new sensitive and selective method for the determination of sultamicillin in bulk and pharmaceutical preparations has been developed. Methods: The developed method is based on the derivatization of sultamicillin with 4-chloro-7-nitrobenzofurazan in pH 9.0 borate buffer at 70ºC for 60 min. The derivative was measured spectrophotometrically at 432 nm after extraction with chloroform. Results: The method was validated by linearity, LOD, LOQ, accuracy, precision and recovery parameters. The linear range for sultamicillin was 10-50 µg.mL-1 with a correlation coefficient of 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.47 and 4.41 µg/mL-1 , respectively. Conclusion: The suggested method was validated, and can be applied for routine sultamicillin analysis in dosage formsfor industrial analysis.
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