UV protolysis of aromatic amino acids and related dipeptides and tripeptides

Khoroshilova, E. V.; Repeyev, Yuri A.; Nikogosyan, David N.

doi:10.1016/1011-1344(90)85153-n

Cited by 44 publications

(10 citation statements)

References 11 publications

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“…In our study, the difference in LDH enzymatic activity between the direct treatment and indirect treatment can be attributed to the difference in the RS in the two modes. Compared to the indirect treatment, besides the effects of the long-lived RS (hydrogen peroxide, ozone, and nitrate ions in the PBS solution), there are short-lived RS such as OH˙, O 2 − , and O 2 ( 1 Δg) as well as UV in the direct treatment 31 33 34 35 36 . Hence, the RS may be responsible for the slightly larger effects in the direct treatment.…”

Section: Resultsmentioning

confidence: 99%

Effects and Mechanism of Atmospheric-Pressure Dielectric Barrier Discharge Cold Plasmaon Lactate Dehydrogenase (LDH) Enzyme

Zhang

Shen

et al. 2015

Sci Rep

129

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Proteins are carriers of biological functions and the effects of atmospheric-pressure non-thermal plasmas on proteins are important to applications such as sterilization and plasma-induced apoptosis of cancer cells. Herein, we report our detailed investigation of the effects of helium-oxygen non-thermal dielectric barrier discharge (DBD) plasmas on the inactivation of lactate dehydrogenase (LDH) enzyme solutions. Circular dichroism (CD) and dynamic light scattering (DLS) indicate that the loss of activity stems from plasma-induced modification of the secondary molecular structure as well as polymerization of the peptide chains. Raising the treatment intensity leads to a reduced alpha-helix content, increase in the percentage of the beta-sheet regions and random sequence, as well as gradually decreasing LDH activity. However, the structure of the LDH plasma-treated for 300 seconds exhibits a recovery trend after storage for 24 h and its activity also increases slightly. By comparing direct and indirect plasma treatments, plasma-induced LDH inactivation can be attributed to reactive species (RS) in the plasma, especially ones with a long lifetime including hydrogen peroxide, ozone, and nitrate ion which play the major role in the alteration of the macromolecular structure and molecular diameter in lieu of heat, UV radiation, and charged particles.

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Section: Resultsmentioning

confidence: 99%

Effects and Mechanism of Atmospheric-Pressure Dielectric Barrier Discharge Cold Plasmaon Lactate Dehydrogenase (LDH) Enzyme

Zhang

Shen

et al. 2015

Sci Rep

129

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“…Since proteins strongly absorb ~280 nm or higher wavelengths, UV-B can affect the aromatic amino acids such as tyrosine, phenylalanine, and tryptophan [89]. Proteins can undergo photomodification directly through photooxidation reactions or indirectly by photosensitized production of active oxygen species and free radicals.…”

Section: Uv Induced Damage and Cell Deathmentioning

confidence: 99%

UV-Induced Cell Death in Plants

Nawkar

Maibam

Park

et al. 2013

IJMS

206

128

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Plants are photosynthetic organisms that depend on sunlight for energy. Plants respond to light through different photoreceptors and show photomorphogenic development. Apart from Photosynthetically Active Radiation (PAR; 400–700 nm), plants are exposed to UV light, which is comprised of UV-C (below 280 nm), UV-B (280–320 nm) and UV-A (320–390 nm). The atmospheric ozone layer protects UV-C radiation from reaching earth while the UVR8 protein acts as a receptor for UV-B radiation. Low levels of UV-B exposure initiate signaling through UVR8 and induce secondary metabolite genes involved in protection against UV while higher dosages are very detrimental to plants. It has also been reported that genes involved in MAPK cascade help the plant in providing tolerance against UV radiation. The important targets of UV radiation in plant cells are DNA, lipids and proteins and also vital processes such as photosynthesis. Recent studies showed that, in response to UV radiation, mitochondria and chloroplasts produce a reactive oxygen species (ROS). Arabidopsis metacaspase-8 (AtMC8) is induced in response to oxidative stress caused by ROS, which acts downstream of the radical induced cell death (AtRCD1) gene making plants vulnerable to cell death. The studies on salicylic and jasmonic acid signaling mutants revealed that SA and JA regulate the ROS level and antagonize ROS mediated cell death. Recently, molecular studies have revealed genes involved in response to UV exposure, with respect to programmed cell death (PCD).

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“…As reported by Khoroshilova et al [115] {Khoroshilova, 1990 #8013}, the disulfide bond in cystine and aromatic amino acids can be photolysis by UV. Based on the result of Surowsky et al {97}, quenching occurred in tryptophan emission spectrum after cold plasma treatment of peroxide and polyphenol oxide enzymes, which was attributed to tryptophan oxidation and also decreased distance between tryptophan and hem resulting in higher energy transfer between them.…”

Section: Impact Of Non-thermal Processing On Amino Acidsmentioning

confidence: 87%

Bridging the Knowledge Gap for the Impact of Non-Thermal Processing on Proteins and Amino Acids

Esteghlal

Gahruie

Niakousari

et al. 2019

Foods

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Proteins represent one of the major food components that contribute to a wide range of biophysical functions and dictate the nutritional, sensorial, and shelf-life of food products. Different non-thermal processing technologies (e.g., irradiation, ultrasound, cold plasma, pulsed electric field, and high-pressure treatments) can affect the structure of proteins, and thus their solubility as well as their functional properties. The exposure of hydrophobic groups, unfolding followed by aggregation at high non-thermal treatment intensities, and the formation of new bonds have been reported to promote the modification of structural and functional properties of proteins. Several studies reported the reduction of allergenicity of some proteins after the application of non-thermal treatments. The composition and concentration of free amino acids could be changed after non-thermal processing, depending on the processing time and intensity. The present review discusses the effects of different non-thermal treatments on protein properties in detail, and highlights the opportunities and disadvantages of these technologies in relation to protein functionality.

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UV protolysis of aromatic amino acids and related dipeptides and tripeptides

Cited by 44 publications

References 11 publications

Effects and Mechanism of Atmospheric-Pressure Dielectric Barrier Discharge Cold Plasmaon Lactate Dehydrogenase (LDH) Enzyme

Effects and Mechanism of Atmospheric-Pressure Dielectric Barrier Discharge Cold Plasmaon Lactate Dehydrogenase (LDH) Enzyme

UV-Induced Cell Death in Plants

Bridging the Knowledge Gap for the Impact of Non-Thermal Processing on Proteins and Amino Acids

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