UV-nanoimprint lithography as a tool to develop flexible microfluidic devices for electrochemical detection

Chen, Juhong; Zhou, Yiliang; Wang, Danhui; He, Fei; Rotello, Vincent M.; Carter, Kenneth R.; Watkins, James J.; Nugen, Sam R.

doi:10.1039/c5lc00515a

Cited by 86 publications

(65 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As compared to most of the additive methods discussed above, subtractive manufacturing methods based on lithography provide similar resolution for fabricating micro‐ and nano‐scale structures, but these methods are typically limited to planar or 2.5D structures . Multiphoton ablation (MPA), a method that utilize ultrafast lasers to remove material, is capable of creating embedded hollow microfeatures such as channels, grooves in glass and polymer substrates .…”

Section: Introductionmentioning

confidence: 99%

Hybrid Laser Printing of 3D, Multiscale, Multimaterial Hydrogel Structures

Kunwar

Xiong

Zhu

et al. 2019

Advanced Optical Materials

View full text Add to dashboard Cite

Fabrication of multiscale, multimaterial 3D structures at high resolution is difficult using current technologies. This is especially significant when working with mechanically weak hydrogels. Here, a new hybrid laser printing (HLP) technology is reported to print complex, multiscale, multimaterial, 3D hydrogel structures with microscale resolution. This technique utilizes sequential additive and subtractive modes of fabrication, that are typically considered as mutually exclusive due to differences in their material processing conditions. Further, compared to current laser writing systems that enforce stringent processing depth limits, HLP is shown to fabricate structures at any depth inside the material. As a proof‐of‐principle, a Mayan pyramid with embedded cube frame is printed using synthetic polyethylene glycol diacrylate (PEGDA) hydrogel. Printing of ready‐to‐use open‐well chips with embedded microchannels is also demonstrated using PEGDA and gelatin methacrylate (GelMA) hydrogels for potential applications in biomedical sciences. Next, HLP is used in additive–additive modes to print multiscale 3D structures spanning in size from centimeter to micrometers within minutes, which is followed by printing of 3D, multimaterial, multiscale structures using this technology. Overall, this work demonstrates that HLP's fabrication versatility can potentially offer a unique opportunity for a range of applications in optics and photonics, biomedical sciences, microfluidics, etc.

show abstract

Section: Introductionmentioning

confidence: 99%

Hybrid Laser Printing of 3D, Multiscale, Multimaterial Hydrogel Structures

Kunwar

Xiong

Zhu

et al. 2019

Advanced Optical Materials

View full text Add to dashboard Cite

show abstract

“…The electrochemical cell includes an inkjet‐printed gold counter electrode (CE), working electrode (WE), and silver reference electrode (RE). Reproduced with permission . Copyright 2015, the Royal Society of Chemistry.…”

Section: Immunoassaysmentioning

confidence: 99%

“…Chen et al demonstrated how the use of printing techniques can enable the mass production and commercial viability of a microfluidic biosensor (Figure b) (CFM, IJP). The authors fabricated microchannels in a polyethylene terephthalate (PET) substrate using nanoimprint lithography .…”

Section: Immunoassaysmentioning

confidence: 99%

Printed Microfluidics

Dixon

Lamanna

Wheeler

2017

Adv Funct Materials

View full text Add to dashboard Cite

Microfluidics has become an important tool that is useful for a wide range of applications. A drawback for microfluidics is that many of the techniques that are commonly used to fabricate devices are not widely accessible, not scalable to high‐volume manufacturing processes, or both. Recently, a number of printing strategies that were originally developed for other applications have been applied to microfluidic device fabrication. These techniques, which include inkjet printing (IJP), screen printing (SP), and solid wax printing (SWP), are proposed to have a transformative effect on the field. Here microfluidics and printing, are introduced and a list of favorite examples is provided that highlights the accessibility and scalability that the combination is bringing to the field.

show abstract

“…This slow time to answer creates an urgent need to develop rapid and reliable methods to detect bacteria in environmental, food, and water matrices. Compounding the challenge is the desirability for these methods to be useful in in resource‐limited settings . A colorimetric assay that does not rely upon advanced instrumentation and skilled operators would be a promising method for rapid detection of bacterial pathogens.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Detection of Escherichia coli Based on the Enzyme-Induced Metallization of Gold Nanorods

Chen

Jackson

Rotello

et al. 2016

Small

Self Cite

138

View full text Add to dashboard Cite

A novel enzyme-induced metallization colorimetric assay was developed to monitor and measure beta-galactosidase (β-gal) activity, and was further employed for colorimetric bacteriophage (phage)-enabled detection of Escherichia coli (E. coli). This assay relies on enzymatic reaction-induced silver deposition on the surface of gold nanorods (AuNRs). In the presence of β-gal, the substrate p-aminophenyl β-D-galactopyranoside (PAPG) is hydrolyzed to produce p-aminophenol (PAP). Reduction of silver ions by PAP generates a silver shell on the surface of AuNRs, resulting in the blue shift of the longitudinal localized surface plasmon resonance (LSPR) peak and multi-color changes of the detection solution from light green to orange-red. Under optimized conditions, the detection limit for β-gal was 128 pM, which was lower than the conventional colorimetric assay. Additionally, the assay had a broader dynamic range for β-gal detection. The specificity of this assay for the detection of β-gal was demonstrated against several protein competitors. Additionally, this technique was successfully applied to detect E. coli bacteria cells in combination with bacteriophage infection. Due to the simplicity and short incubation time of this enzyme-induced metallization colorimetric method, the assay is well suited for the detection of bacteria in low-resource settings.

show abstract

UV-nanoimprint lithography as a tool to develop flexible microfluidic devices for electrochemical detection

Cited by 86 publications

References 47 publications

Hybrid Laser Printing of 3D, Multiscale, Multimaterial Hydrogel Structures

Hybrid Laser Printing of 3D, Multiscale, Multimaterial Hydrogel Structures

Printed Microfluidics

Colorimetric Detection of Escherichia coli Based on the Enzyme-Induced Metallization of Gold Nanorods

Contact Info

Product

Resources

About