UV LASER INDUCED RNA‐PROTEIN CROSSLINKS AND RNA CHAIN BREAKS IN TOBACCO MOSAIC VIRUS RNA <i>in situ</i>

Добров, Е. Н.; Arbieva, Z. Kh.; Timofeeva, E.K.; Esenaliev, Rinat O.; Oraevsky, Alexander A.; Nikogosyan, David N.

doi:10.1111/j.1751-1097.1989.tb08429.x

Cited by 28 publications

(22 citation statements)

References 14 publications

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“…With double-stranded D N A monophotonic ssb formation has been observed in low yields (Table 7) using low intensity continuous U V irradiation (Zavigelsky et al, 1984;Dobrov et al, 1986Dobrov et al, , 1989Croke et al, 1988;Bothe et al, 1990). The same effect has also been reported for single-stranded DNA and polynucleotides (Table 7).…”

Section: Monophotonic Strand Break Formationsupporting

confidence: 60%

LASER‐INDUCED STRAND BREAK FORMATION IN DNA and POLYNUCLEOTIDES

Schulte‐Frohlinde

Simic

Görner

1990

Photochem & Photobiology

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Section: Monophotonic Strand Break Formationsupporting

confidence: 60%

LASER‐INDUCED STRAND BREAK FORMATION IN DNA and POLYNUCLEOTIDES

Schulte‐Frohlinde

Simic

Görner

1990

Photochem & Photobiology

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“…Finally, Dobrov et al. investigated RNA–protein crosslinking in tobacco mosaic virus with a 10 ns Nd:YAG laser (10 MW cm −2 ) and with a 23 ps Nd:YAG laser (1.5 GW cm −2 ) (8). Irradiation of samples with ∼5–100% of the maximum intensity from each laser individually yielded intensity‐independent crosslinking quantum yields, but the quantum yield using the nanosecond laser was 18 times lower than using the picosecond laser.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Femtosecond Laser and Continuous Wave UV Sources for Protein–Nucleic Acid Crosslinking

Fecko¹,

Munson²,

Saunders³

et al. 2007

Photochem & Photobiology

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Crosslinking proteins to the nucleic acids they bind affords stable access to otherwise transient regulatory interactions. Photochemical crosslinking provides an attractive alternative to formaldehyde-based protocols, but irradiation with conventional UV sources typically yields inadequate product amounts. Crosslinking with pulsed UV lasers has been heralded as a revolutionary technique to increase photochemical yield, but this method had only been tested on a few protein-nucleic acid complexes. To test the generality of the yield enhancement, we have investigated the benefits of using approximately 150 fs UV pulses to crosslink TATA-binding protein, glucocorticoid receptor and heat shock factor to oligonucleotides in vitro. For these proteins, we find that the quantum yields (and saturating yields) for forming crosslinks using the high-peak intensity femtosecond laser do not improve on those obtained with low-intensity continuous wave (CW) UV sources. The photodamage to the oligonucleotides and proteins also has comparable quantum yields. Measurements of the photochemical reaction yields of several small molecules selected to model the crosslinking reactions also exhibit nearly linear dependences on UV intensity instead of the previously predicted quadratic dependence. Unfortunately, these results disprove earlier assertions that femtosecond pulsed laser sources provide significant advantages over CW radiation for protein-nucleic acid crosslinking.

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“…, 1988). A value of @& = 1.7 X lo-' has been found by Dobrov et a/. (1989) for R N A using ns laser pulses (266 nm, IL = 4.7 x 10' Wcm-').…”

Section: Introductionmentioning

confidence: 88%

“…The study of the mechanism of strand break formation in D N A and polynucleotides, induced by pulsed high-intensity laser light, is of increasing interest (Gurzadyan et a/., 1982;Nikogosyari and Gurzadyan, 1984;Zavilgelsky et a/., 1984;Schulte-Frohlinde, 1986a, 1986bDobrov et a/., 1986Dobrov et a/., , 1989Croke et a/., 1988). Pulsed UVlaser light allows one to investigate the chemical reactions following ionization of the base moiety of nucleic acids (Schulte-Frohlinde et a/.…”

Section: Introductionmentioning

confidence: 99%

SINGLE‐ and DOUBLE‐STRAND BREAK FORMATION IN DOUBLE‐STRANDED DNA UPON NANOSECOND LASER‐INDUCED PHOTOIONIZATION

Bothe¹,

Görner²,

Opitz³

et al. 1990

Photochem & Photobiology

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Double-stranded (ds) calf thymus DNA (0.4 mM), excited by 20 ns laser pulses at 248 nm, was studied in deoxygenated aqueous solution at room temperature and pH 6.7 in the presence of a sodium salt (10 mM). The quantum yields for the formation of hydrated electrons (phi c-), single-strand breaks (phi ssb) and double-strand breaks (phi dsb) were determined for various laser pulse intensities (IL). phi c- and phi ssb increase linearly with increasing IL; however, phi ssb has a tendency to reach saturation at high IL (greater than 5 X 10(6) Wcm-2). The ratio phi ssb/phi c-, representing the number of ssb per radical cation, is about 0.08 at IL less than or equal to 5 X 10(6) Wcm-2. For comparison, the number of ssb per OH radical reacting with dsDNA is 0.22. On going from argon to N2O saturation, phi ssb and phi dsb become larger by factors of approximately 5 and 10-15, respectively. This enhancement is produced by attack on DNA bases by OH radicals generated by N2O-scavenging of the photoelectrons. While phi ssb is essentially independent of the dose (Etot), phi dsb depends linearly on Etot in both argon- and N2O-saturated solutions. The linear dependence of phi dsb implies a square dependence of the number of dsb on Etot. This portion of dsb formation is explained by the occurrence of two random ssb, generated within a critical distance (h) in opposite strands. For both argon- and N2O-saturated solutions h was found to be of the order of 40-70 phosphoric acid diester bonds. On addition of electron scavengers such as 2-chloroethanol (or N2O plus t-butanol), phi dsb is similar to that in neat, argon-saturated solutions. Thus, hydrated electrons are not involved in the chemical pathway leading to laser-pulse-induced dsb of DNA.

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UV LASER INDUCED RNA‐PROTEIN CROSSLINKS AND RNA CHAIN BREAKS IN TOBACCO MOSAIC VIRUS RNA in situ

Cited by 28 publications

References 14 publications

LASER‐INDUCED STRAND BREAK FORMATION IN DNA and POLYNUCLEOTIDES

LASER‐INDUCED STRAND BREAK FORMATION IN DNA and POLYNUCLEOTIDES

Comparison of Femtosecond Laser and Continuous Wave UV Sources for Protein–Nucleic Acid Crosslinking

SINGLE‐ and DOUBLE‐STRAND BREAK FORMATION IN DOUBLE‐STRANDED DNA UPON NANOSECOND LASER‐INDUCED PHOTOIONIZATION

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