UV induces reactive oxygen species, damages sperm, and impairs fertilisation in the sea urchin Anthocidaris crassispina

Lü, Xiaoying; Wu, Rongben

doi:10.1007/s00227-005-0049-7

Cited by 34 publications

(19 citation statements)

References 39 publications

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“…In the present study, the exposure of catla to different doses of UV-B radiation substantially increased the TBARS level indicating lipid damage. Higher level of lipid peroxidation was reported in UV-B irradiated sea chub Girella laevifrons [3], rohu [6], freshwater prawn Macrobrachium olfersi [45] and sea urchin, Strongylocentrotus droebachiensis sperm [46]. Fish contain high amount of polyunsaturated fatty acids that are easily modified by peroxidation and hence, it has been used a biomarker of pollution [41].…”

Section: Discussionmentioning

confidence: 98%

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Singh

Sharma

Chakrabarti

2015

Journal of Photochemistry and Photobiology B: Biology

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Section: Discussionmentioning

confidence: 98%

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Singh

Sharma

Chakrabarti

2015

Journal of Photochemistry and Photobiology B: Biology

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“…Sperm also have greater surface area to volume ratios and high levels of polyunsaturated fatty acids (PUFAs) in their cellular and intracellular membranes making them prone to lipid peroxidation and low DNA repair capabilities (Lu and Wu, 2005b). Here we present support for limited antioxidant capacities in sperm, but found low levels of oxidative damage to gametes overall providing no support for higher susceptibility of the male gamete in this species.…”

Section: Discussionmentioning

confidence: 68%

Pollutant resilience in embryos of the Antarctic sea urchin Sterechinus neumayeri reflects maternal antioxidant status

2015

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“…Similarly, Lu and Wu (2005) showed that MMP of sea urchin sperm was more sensitive to UVR (ultraviolet radiation) than membrane integrity. Graham (2001) also observed that measuring multiple attributes simultaneously on individual sperm in a population should provide a better estimate of the percentage of sperm that could fertilize an oocyte.…”

Section: Mitochondrial Membrane Potential (Mmp)mentioning

confidence: 99%

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Goïc

Hégaret

Fabioux

et al. 2013

Aquat. Living Resour.

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-The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only penetrates cells with intact membranes. Cell mortality was measured with propidium iodide (PI), which penetrates cells with membrane damage. Mitochondrial membrane potential, used as an estimate of mitochondrial function, was measured using JC-1 dye, which selectively enters into mitochondria and reversibly changes colour from green to orange as the membrane potential increases. To assess the effect of toxic algae on oyster sperm, broodstock (ripe oysters) were fed toxic (A. catenella) or non toxic (Heterocapsa triquetra) dinoflagellates at 250 cell ml −1 for 9 days. After this exposure period, mature oysters were stripped and cellular responses of sperm analysed. Average DNA staining, as measured by SYBR-14, appeared lower and more variable in gametes from A. catenella-exposed oysters than in those from control oysters fed H. triquetra. Additionally, mitochondrial membrane potential of sperm from A. catenella-exposed oysters was significantly higher (1.5 fold) than that of sperm produced by oysters fed H. triquetra. Both the increase of mitochondrial membrane potential and the modification of DNA structure can be expected to impact spermatozoa ability to fertilize oocytes and could thus impact related reproductive processes.

show abstract

UV induces reactive oxygen species, damages sperm, and impairs fertilisation in the sea urchin Anthocidaris crassispina

Cited by 34 publications

References 39 publications

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation

Pollutant resilience in embryos of the Antarctic sea urchin Sterechinus neumayeri reflects maternal antioxidant status

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

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