UV-induced self-aggregation of E . coli after low and medium pressure ultraviolet irradiation

Kollu, Kerim; Örmeci, Banu

doi:10.1016/j.jphotobiol.2015.04.013

Cited by 19 publications

(12 citation statements)

References 25 publications

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“…The proportion between small clusters and total cells increased during the experiment, regardless of the disinfection treatment and of the regrowth tests. This is in agreement with what was observed in previous studies where aggregation, or the selection towards small clusters, was a common response of bacterial communities when exposed to a stress such as UV light exposure (Kollu and Örmeci, 2015), predation (Corno and Jurgens, 2006), antibiotic pressure (Corno et al, 2014), and chemical disinfection (Di Cesare et al, 2016). However, a significant increase of the relative abundance of small clusters as a consequence of the disinfection treatment was not observed.…”

Section: Discussionsupporting

confidence: 92%

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

et al. 2017

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Peracetic acid (PAA) is an organic compound used efficiently as disinfectant in wastewater treatments. Yet, at low doses it may cause selection; thus, the effect of low doses of PAA on Enterococcus faecium as a proxy of human-related microbial waste was evaluated. Bacteria were treated with increasing doses of PAA (from 0 to 25 mg L min) and incubated in regrowth experiments under non-growing, limiting conditions and under growing, favorable conditions. The changes in bacterial abundance, in bacterial phenotype (number and composition of small cell clusters), and in the abundance of an antibiotic resistance gene (ARG) was evaluated. The experiment demonstrated that the selected doses of PAA efficiently removed enterococci, and induced a long-lasting effect after PAA inactivation. The relative abundance of small clusters increased during the experiment when compared with that of the inoculum. Moreover, under growing favorable conditions the relative abundance of small clusters decreased and the number of cells per cluster increased with increasing PAA doses. A strong stability of the measured ARG was found, not showing any effect during the whole experiment. The results demonstrated the feasibility of low doses of PAA to inactivate bacteria. However, the stress induced by PAA disinfection promoted a bacterial adaptation, even if potentially without affecting the abundance of the ARG.

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Section: Discussionsupporting

confidence: 92%

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

et al. 2017

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“…The tailing phenomenon was observed during UV irradiation. Tailing could be attributed to the occurrence of microbial self‐aggregates caused by the change in surface characteristics (lipids) and the production of extracellular polymers during UV irradiation (Kollu and Örmeci ). Similar results were reported previously (Blatchley et al .…”

Section: Discussionmentioning

confidence: 99%

Effects of ultraviolet disinfection on antibiotic-resistantEscherichia colifrom wastewater: inactivation, antibiotic resistance profiles and antibiotic resistance genes

Zhang

Wang

et al. 2017

J Appl Microbiol

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“…The underlying mechanism is still mysterious. Cell aggregation has been proposed to be an important adaptive strategy ensuring the survival and growth of bacteria in adverse environments [ 34 , 35 ]. Thus, we inferred that aggregation induced by HicA may be an adaptation adopted by E. coli cells to cope with the stress triggered by toxin overproduction.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of the HicAB Toxin-Antitoxin System in the Opportunistic Pathogen Pseudomonas aeruginosa

Shen

et al. 2016

Toxins

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Toxin-antitoxin (TA) systems are small genetic modules that are widely distributed in the genomes of bacteria and archaea and have been proposed to fulfill numerous functions. Here, we describe the identification and characterization of a type II TA system, comprising the hicAB locus in the human opportunistic pathogen Pseudomonas aeruginosa. The hicAB locus consists of genes hicA and hicB encoding a toxin and its cognate antitoxin, respectively. BLAST analysis revealed that hicAB is prevalent in approximately 36% of P. aeruginosa strains and locates in the same genomic region. RT-PCR demonstrated that hicAB forms a bicistronic operon that is cotranscribed under normal growth conditions. Overproduction of HicA inhibited the growth of Escherichia coli, and this effect could be counteracted by co-expression of HicB. The Escherichia coli kill/rescue assay showed that the effect of HicA is bacteriostatic, rather than bactericidal. Deletion of hicAB had no effect on the biofilm formation and virulence of P. aeruginosa in a mice infection model. Collectively, this study presents the first characterization of the HicAB system in the opportunistic pathogen P. aeruginosa.

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UV-induced self-aggregation of E . coli after low and medium pressure ultraviolet irradiation

Cited by 19 publications

References 25 publications

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

Effects of ultraviolet disinfection on antibiotic-resistantEscherichia colifrom wastewater: inactivation, antibiotic resistance profiles and antibiotic resistance genes

Identification and Characterization of the HicAB Toxin-Antitoxin System in the Opportunistic Pathogen Pseudomonas aeruginosa

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