UV‐induced DNA damage initiates release of MMP‐1 in human skin

Dong, Kelly; Damaghi, Niusha; Picart, Stephanie D.; Markova, Nedialka G.; Obayashi, Kei; Okano, Yasuaki; Hashimoto, Masaki; Grether‐Beck, Susanne; Krutmann, Jean; Smiles, Kenneth A.; Yarosh, Daniel B.

doi:10.1111/j.1600-0625.2008.00747.x

Cited by 162 publications

(160 citation statements)

References 34 publications

Supporting

Mentioning

154

Contrasting

Order By: Relevance

“…The ROS stimulated MMPs were detected accompanying with broken connective tissue in photoaged skin 4. Considerable researches have reported that antioxidative botanicals are potential to prevent skin damages caused by UV irradiation.…”

Section: Discussionmentioning

confidence: 99%

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Sun

Park

Hwang

et al. 2016

J Cellular Molecular Medi

View full text Add to dashboard Cite

Solar ultraviolet (UV) radiation‐induced reactive oxidative species is mainly responsible for the development of photoageing. Rosmarinic acid was one of the main bioactive components detected in Thymus vulgaris (TV) we extracted. In this study, UVB‐induced skin damages have been shown to be ameliorated by treatment with TV in hairless mice (HR‐1) skin, demonstrated by decreased matrix metalloproteinases (MMPs) and increased collagen production. However, the underlying molecular mechanism on which TV acted was unclear. We examined the photoprotective effects of TV against UVB and elucidated the molecular mechanism in normal human dermal fibroblasts. Thymus vulgaris remarkably prevented the UVB‐induced reactive oxygen species and lactate dehydrogenase. Dose‐dependent increase in glutathione, NAD(P)H: quinone oxidoreductase1 and heme oxygenase‐1, by TV was confirmed by increased nuclear accumulation of Nrf2. Furthermore, 5‐Methoxyindole‐2‐carboxylic acid was introduced as a specific inhibitor of dihydrolipoyl dehydrogenase (DLD). We demonstrated that Nrf2 expression was regulated by DLD, which was a tricarboxylic acid cycle‐associated protein that decreased after UVB exposure. Besides, TV significantly diminished UVB induced phosphorylation of mitogen activated protein kinases pathway, containing extracellular signal‐regulated kinase, Jun N‐terminal kinase and p38, which consequently reduced phosphorylated c‐fos and c‐jun. Our results suggest that TV is a potential botanical agent for use against UV radiation‐induced oxidative stress mediated skin damages.

show abstract

Section: Discussionmentioning

confidence: 99%

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Sun

Park

Hwang

et al. 2016

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…7) Among MMPs, MMP-1 is the most responsible for the degradation of type 1 collagen in the skin. [8][9][10] Collagen degradation and inhibition of collagen production impair the structural integrity of the skin during photoaging. Therefore, control of collagen metabolism may be useful for a variety of therapeutic and cosmetic applications.…”

mentioning

confidence: 99%

A Glycosidic Spinasterol from Koreana stewartia Promotes Procollagen Production and Inhibits Matrix Metalloproteinase-1 Expression in UVB-Irradiated Human Dermal Fibroblasts

Lee

et al. 2011

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

UV irradiation, particularly that of UVB (290-320 nm), causes various adverse effects to human skin, the most serious being skin cancer and premature skin aging (photoaging). [1][2][3] The functional properties of skin depend on the integrity of collagens, which are the most abundant structural proteins in dermis. 4) Type 1 collagen, which accounts for 70-90% of all collagen, is synthesized by fibroblasts as a soluble precursor, type 1 procollagen, secreted and proteolytically processed to form insoluble collagen fibers in the dermis. 5,6) The production and fibrillar organization of collagen in the skin are reduced by UV irradiation whereas gene expression of matrix metalloproteinases (MMPs) is increased. 7) Among MMPs, MMP-1 is the most responsible for the degradation of type 1 collagen in the skin. [8][9][10] Collagen degradation and inhibition of collagen production impair the structural integrity of the skin during photoaging. Therefore, control of collagen metabolism may be useful for a variety of therapeutic and cosmetic applications.Recently, a variety of herbs and plants have traditionally been used in cosmetic applications for the treatment of collagen synthesis and collagenase inhibition. [11][12][13][14][15] It has been widely reported that natural compounds from plants are potential sources of MMP inhibitors, which may prevent or mitigated UV-induced skin aging. [16][17][18] Stewartia koreana (Theaceae), a deciduous tree, is native to Korea and grows throughout the whole area of Korea. It prefers a partly shaded to full sun area, and favors a rich, moist, well-drained, and acidic loam. The leaves are dark green, elliptical, and mildly serrulate, and alternately arranged on stem. In autumn, the leaves turn a bright red to reddish purple. Usually in July, white solitary flowers bloom with 5-6 petals and yellow stamens. The flowers open wider with a more flattened look than most other species of Stewartia. The fruits ripen as an inconspicuous pentagon-shaped one in October.The bark is well exfoliated, ranging in color from soft grays and tans to orange and reddish browns. 19) We previously reported that Stewarita koreana extract induced angiogenesis and extracellular matrix (ECM) remodeling in a mouse model. It has also been reported that alcohol extracts from Stewartia koreana stimulate vasculization in vivo 20) but there is no report for the individual components of the plant. In addition, our preliminary experiments showed that the alcohol extracts were very effective in restoring wounds on punched skin of the back of mice. Therefore, this study was initiated to determine the principal components that manifest the biological activity. We isolated a number of compounds from Stewarita koreana extract. In the present study, we identified an active compound, 3-O-b-D-glucopyranosylspinasterol (spinasterol-Glc), and investigated its effect on the levels of collagen and on the expression of MMP-1 in UVB-treated fibroblast cells. We found that spinasterol-Glc inhibits collagen degradation in UVB-induced fib...

show abstract

“…It has been reported that the culture medium of UVB-irradiated keratinocytes stimulates MMP-1 release from fibroblasts more efficiently than the direct irradiation of the fibroblasts. 10,11) The primary mechanism by which UV irradiation initiates molecular responses in human skin is through the photochromic generation of reactive oxygen species (ROS). ROS cause oxidative damage and decrease the levels of non-enzymatic antioxidants, such as glutathione (GSH), resulting in the subsequent activation of complex signalling pathways and MMP induction.…”

mentioning

confidence: 99%

The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Maruki‐Uchida

Kurita

Sugiyama

et al. 2013

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The use of naturally occurring botanicals with substantial antioxidant activity to prevent photoageing is receiving increasing attention. We have previously identified piceatannol and scirpusin B, which is a dimer of piceatannol, as strong antioxidants that are present in passion fruit (Passiflora edulis) seeds. In the present study, the effects of passion fruit seed extract, piceatannol, and scirpusin B on human keratinocytes were investigated. The passion fruit seed extract and piceatannol upregulated the glutathione (GSH) levels in keratinocytes in a dose-dependent manner, indicating that piceatannol is an active component of the passion fruit seed extract in keratinocytes. The pretreatment with piceatannol also supressed the UVB-induced generation of reactive oxygen species (ROS) in the keratinocytes. In addition, the transfer of the medium from the UVBirradiated keratinocytes to non-irradiated fibroblasts enhanced matrix-metalloproteinase (MMP)-1 activity, and this MMP-1 induction was reduced when the keratinocytes were pretreated with piceatannol. These results suggest that piceatannol attenuates the UVB-induced activity of MMP-1 along with a reduction of ROS generation in keratinocytes. Thus, piceatannol and passion fruit seed extract containing high amounts of piceatannol are potential anti-photoageing cosmetic ingredients.Key words piceatannol; passion fruit seed; keratinocyte; ultraviolet; reactive oxygen species; matrix-metalloproteinase Solar UV irradiation damages human skin causing it to age prematurely, a process referred to as photoageing, and to develop the characteristics of thickened epidermis and increased melanogenesis.1-3) In contrast, intrinsic (chronological) ageing is characterised by skin atrophy accompanied by a loss of elasticity and reduced metabolic activity. [4][5][6][7][8] A hallmark of photoageing is the disorganisation of collagen, the major structural component of the skin. Collagen is produced mainly by dermal fibroblasts and is degraded by the matrix metalloproteinases (MMPs) that are secreted by various cells, including keratinocytes, fibroblasts, and inflammatory cells.2,8,9) UV irradiation, particularly UVB (290-320 nm) and UVA (315-400 nm), induces the expression of matrix metalloproteinase-1 (MMP-1) in the fibroblasts, which is mainly responsible for the degradation of the dermal collagen in the ageing process of human skin. Therefore, UV-induced MMP-1 is considered to contribute substantially to the connective tissue damage that causes severe collagen deficiency and wrinkling during photoageing.2,8) Although keratinocytes do not produce MMP-1 in response to UVB irradiation, damaged epidermal keratinocytes indirectly play important roles in the release of MMP-1. It has been reported that the culture medium of UVB-irradiated keratinocytes stimulates MMP-1 release from fibroblasts more efficiently than the direct irradiation of the fibroblasts. 10,11)The primary mechanism by which UV irradiation initiates molecular responses in human skin is through the photochromic genera...

show abstract

UV‐induced DNA damage initiates release of MMP‐1 in human skin

Cited by 162 publications

References 34 publications

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

A Glycosidic Spinasterol from Koreana stewartia Promotes Procollagen Production and Inhibits Matrix Metalloproteinase-1 Expression in UVB-Irradiated Human Dermal Fibroblasts

The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Contact Info

Product

Resources

About