UV induced changes in proteome of rats plasma are reversed by dermally applied cannabidiol

Abstract

UV radiation is known to induce a multiple changes in the metabolism of skin-building cells, what can affect the functioning not only neighboring cells, but also, following signal transduction releasing into the blood vessels, the entire body. Therefore, the aim of this study was to analyze the proteomic disturbances occurred in plasma of chronically UVA/UVB irradiated rats and define the effect on these changes of skin topically applied cannabidiol (CBD). Obtained results showed significant changes in the expression of numerous anti-inflammatory and signaling proteins including: NFκB inhibitor, 14-3-3 protein, protein kinase C, keratin, and protein S100 after UV irradiation and CBD treatment. Moreover, the effects of UVA and UVB were manifested by increased level of lipid peroxidation products—protein adducts formation. CBD partially prevented all of these changes, but in a various degree depending on the UV radiation type. Moreover, topical treatment with CBD resulted in the penetration of CBD into the blood and, as a consequence, in direct modifications to the plasma protein structure by creating CBD adducts with molecules, such as proline-rich protein 30, transcription factor 19, or N-acetylglucosamine-6-sulfatase, what significantly changed the activity of these proteins. In conclusion, it may be suggested that CBD applied topically may be an effective compound against systemic UV-induced oxidative stress, but its effectiveness requires careful analysis of CBD's effects on other tissues of the living organism.

Photodissociation of leucine-enkephalin protonated peptide: an experimental and theoretical perspective

“…The interaction of biomolecules with ultraviolet (UV) light is of paramount interest due to the pivotal role that such interactions play in fundamental biological processes, 1 ranging from DNA stability and repair [2][3][4][5] to protein function, [6][7][8][9][10][11][12] cellular signaling, 13,14 photosynthesis, 15,16 neurotransmission, 17 immune response, 18,19 or cell cycle regulation. 20 In addition, the interaction of peptides with UV light has signicant implications in many other elds such as the study of the origin of life, 21,22 photodegradation of industrially important polymeric materials 23,24 or photodegradation of biopharmaceuticals that can be exposed to UV light during their manufacturing and administration process.…”

“…The interaction of biomolecules with ultraviolet (UV) light is of paramount interest due to the pivotal role that such interactions play in fundamental biological processes, 1 ranging from DNA stability and repair 2–5 to protein function, 6–12 cellular signaling, 13,14 photosynthesis, 15,16 neurotransmission, 17 immune response, 18,19 or cell cycle regulation. 20…”