UV-enhanced cytotoxicity of thiol-capped CdTe quantum dots in human pancreatic carcinoma cells

Chang, Shuquan; Dai, Yaodong; Kang, Bin; Han, Wei; Mao, Ling; Chen, Da

doi:10.1016/j.toxlet.2009.03.013

Cited by 52 publications

(47 citation statements)

References 35 publications

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“…Notably, the concentration of CdTe QDs required to promote endothelial toxicity (TC 50 ) in our study was much lower than most of that in other cell types (Chen et al 2010;Choi et al 2007,(Su et al 2010, (Su et al 2009;Wu et al 2010), (Chang et al 2009;Chen et al 2010;Choi et al 2007;Lovric et al 2005;Shiohara et al 2004;Tang et al 2008). A possible explanation for this difference is that the tested cell types have different cellular sensitivity to QDs.…”

Section: Discussioncontrasting

confidence: 52%

“…molarity was about 0.227 nM to 227 nM). According to previous reports, the concentration which we tested in our following experiments is similar to the doses that have actually used in in vivo bioimaging (up to hundreds of nanomole) (Ballou et al 2007;Ballou et al 2004;Cai et al 2006;Gao et al 2004;Jayagopal et al 2007;Larson et al 2003;Tada et al 2007) or tested in other in vitro toxicological studies (up to hundreds of nanomole or μg/mL) 20 (Chang et al 2009;Chen et al 2010;Choi et al 2007;Lovric et al 2005;Su et al 2009;Su et al 2010;Tang et al 2008;Wu et al 2010). Then, our cell viability assays (Fig.…”

Section: Discussionmentioning

confidence: 92%

“…Core QDs and QDs with different coatings were also reported to be toxic to primary rat hepatocytes (Shiohara et al 2004). In addition, the cadmium based QDs were demonstrated to induce dramatic cell loss in pancreatic carcinoma cells (PANC-1), rat pheochromocytoma cells (PC12) and murine microglial cells (N9) (Chang et al 2009;Lovric et al 2005). Although these toxicological studies have shown the toxicity results of cell death for some primary or immortalized cell lines (Derfus et al 2004;Su et al 2009;Tang et al 2008), the understanding of QDs cytotoxicity remains limited.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An in vitro study of vascular endothelial toxicity of CdTe quantum dots

Yan¹,

Zhang²,

Xu³

et al. 2011

Toxicology

112

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Quantum dots (QDs), as novel bioimaging and drug delivery agents, are generally introduced into vascular system by injection, and thus directly exposed to vascular endothelial cells (ECs). However, the adverse effects of QDs on ECs are poorly understood. In this study, employing human umbilical vein ECs (HUVECs), we investigated the potential vascular endothelial toxicity of mercaptosuccinic acid (MSA)-capped CdTe QDs in vitro. In the experiment, water-soluble and pH stable CdTe QDs were synthesized; and the cell viability assays showed that CdTe QDs (0.1-100 mu g/mL) dose-dependently decreased the cell viability of HUVECs, indicating CdTe QDs induced significant endothelial toxicity. The flow cytometric and immunofluorescence results revealed that 10 mu g/mL CdTe QDs elicited significant oxidative stress, mitochondrial network fragmentation as well as disruption of mitochondrial membrane potential (Delta psi(m)); whereas ROS scavenger could protect HUVECs from QDs-induced mitochondrial dysfunction. Moreover, upon 24h exposure to 10 mu g/mL CdTe QDs, the apoptotic HUVECs dramatically increased by 402.01%, accompanied with alternative expression of apoptosis proteins, which were upregulation of Bax, down-regulation of Bcl-2, release of mitochondrial cytochrome c and cleavage of caspase-9/caspase-3. These results suggested that CdTe QDs could not only impair mitochondria but also exert endothelial toxicity through activation of mitochondrial death pathway and induction of endothelial apoptosis. Our results provide strong evidences of the direct toxic effects of QDs on human vascular ECs, and reveal that exposure to QDs is a significant risk for the development of cardiovascular diseases. These results also provide helpful guidance on the future safe use and manipulation of QDs to make them more suitable tools in nanomedicine

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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An in vitro study of vascular endothelial toxicity of CdTe quantum dots

Yan¹,

Zhang²,

Xu³

et al. 2011

Toxicology

112

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“…Unrestricted interactions of any kind of materials, including QDs, have the potential to be toxic, especially at higher concentration and/or with enhanced adsorption/internalization. Beyond the changes over cell function, more evident effects are prone to be caused by QDs, including cellular ones such: (i) changes in cell morphology (Chang et al, 2009), (ii) oxidative stress (Lovrić, et al, 2005), (iii) release of heavy metal compounds (Kirchner et al, 2005) and (iv) genetic and epigenetic damage (Choi et al, 2008), as well as local inflammation in live rats, as we have recently reported.…”

Section: Quantum Dots In Biomedical Research 285mentioning

confidence: 75%

Quantum Dots in Biomedical Research

Fontes¹,

Lira²,

Seabra³

et al. 2012

Biomedical Engineering - Technical Applications in Medicine

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“…However, their toxicity in biological environments has not been fully understood [105]. Many groups are currently investigating the toxicity of cadmium-based QDs in vitro and in vivo [106][107][108][109]. Several studies showed no toxicity in small animals at optimal cadmium-based QDs concentration for periods up to several months [13,86].…”

Section: Toxicity Of Quantum Dots In Vitro and In Vivomentioning

confidence: 99%

Aqueous phase synthesis of CdTe quantum dots for biophotonics

Yong

Law

Roy

et al. 2010

Journal of Biophotonics

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Over the past few years, CdTe quantum dots have been demonstrated as powerful probes for biophotonics applications. The aqueous phase synthesis technique remains the best approach to make high quality CdTe QDs in a single‐pot process. CdTe QDs prepared directly in the aqueous phase can have quantum yield as high as 80%. In addition, the surface of CdTe QDs prepared using the aqueous phase technique is functionalized with reactive groups that enable them to be directly conjugated with specific ligands for targeted delivery and sensing. In this contribution, we review recent progress in fabricating aqueous CdTe QDs and exploiting their optical properties in novel approaches to biomedical imaging and sensing applications. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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UV-enhanced cytotoxicity of thiol-capped CdTe quantum dots in human pancreatic carcinoma cells

Cited by 52 publications

References 35 publications

An in vitro study of vascular endothelial toxicity of CdTe quantum dots

An in vitro study of vascular endothelial toxicity of CdTe quantum dots

Quantum Dots in Biomedical Research

Aqueous phase synthesis of CdTe quantum dots for biophotonics

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