UV Dose Governs UV-Polymerized Polyacrylamide Hydrogel Modulus

Sheth, Saahil; Jain, Era; Karadaghy, Amin; Syed, Serajuddaula; Stevenson, Hunter; Zustiak, Silviya Petrova

doi:10.1155/2017/5147482

Cited by 22 publications

(26 citation statements)

References 26 publications

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“…The degree of separation gel crosslinking is affected by the percentage of acrylamide/bis-acrylamide, the quantity of photoinitiator, and the UV-exposure dose 38 . Various conditions of gel percentage and exposure time were tested, with a suitable separation obtained using an 8% gel exposed to an energy density dose of 12,500 mJ/cm 2 in roughly 5 min.…”

Section: Resultsmentioning

confidence: 99%

On-chip protein separation with single-molecule resolution

Zrehen

Ohayon

Huttner

et al. 2020

Sci Rep

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Accurate identification of both abundant and rare proteins hinges on the development of single-protein sensing methods. Given the immense variation in protein expression levels in a cell, separation of proteins by weight would improve protein classification strategies. Upstream separation facilitates sample binning into smaller groups while also preventing sensor overflow, as may be caused by highly abundant proteins in cell lysates or clinical samples. Here, we scale a bulk analysis method for protein separation, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), to the single-molecule level using single-photon sensitive widefield imaging. Single-molecule sensing of the electrokinetically moving proteins is achieved by in situ polymerization of the PAGE in a low-profile fluidic channel having a depth of only ~ 0.6 µm. The polyacrylamide gel restricts the Brownian kinetics of the proteins, while the low-profile channel ensures that they remain in focus during imaging, allowing video-rate monitoring of single-protein migration. Calibration of the device involves separating a set of Atto647N-covalently labeled recombinant proteins in the size range of 14–70 kDa, yielding an exponential dependence of the proteins’ molecular weights on the measured mobilities, as expected. Subsequently, we demonstrate the ability of our fluidic device to separate and image thousands of proteins directly extracted from a human cancer cell line. Using single-particle image analysis methods, we created detailed profiles of the separation kinetics of lysine and cysteine -labeled proteins. Downstream coupling of the device to single-protein identification sensors may provide superior protein classification and improve our ability to analyze complex biological and medical protein samples.

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Section: Resultsmentioning

confidence: 99%

On-chip protein separation with single-molecule resolution

Zrehen

Ohayon

Huttner

et al. 2020

Sci Rep

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“…The increasing of the CMC concentrations resulted in higher modulus of composite gels (Table 1) because more polymer chains in solution lead to a larger number of functional crosslinks. 28 Especially, the modulus when CMC concentrations above 6% were almost two times of those less than or equal to 6% which means the crosslink density in hydrogel networks was relatively high. Therefore, the crosslinking degree of the composite hydrogels increased with increasing content of CMC, and so the strength of the hydrogels increased.…”

Section: Effects Of Cmc Concentration On Mechanical Property and Elecmentioning

confidence: 97%

Preparation of conductive composite hydrogels from carboxymethyl cellulose and polyaniline with a nontoxic crosslinking agent

Fang

Tait

et al. 2017

RSC Adv.

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“…The loss modulus (E″), Figure 5b, Furthermore, the literature indicates that UV exposure time, up to a certain threshold, leads to a higher number of functional crosslinks and enhances the viscoelastic properties of the hydrogels. Longer exposure times allow for the photopolymerization reaction to proceed to completion [49].…”

Section: Stress-strain Behaviormentioning

confidence: 99%

Curing of Cellulose Hydrogels by UV Radiation for Mechanical Reinforcement

et al. 2021

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The use of biomaterials as a replacement for thermoplastic polymers is an environmentally sound strategy. In this work, hydrogels of cellulose isolated from wheat husk were modified by UV irradiation (353 nm) to improve mechanical performance. The cellulose was dissolved with a solvent system N,N-dimethylacetamide/lithium chloride (DMAc/LiCl). Infrared spectroscopy showed that the peak height at 1016 cm−1, associated with the C–O bonds of the glycosidic ring, increases with irradiation time. It was determined that the increase in this signal is related to photodegradation, the product of a progressive increase in exposure to UV radiation. The viscoelastic behavior, determined by dynamic mechanical analysis and rotational rheometry, was taken as the most important parameter of this research, showing that the best results are recorded with 15 min of UV treatment. Therefore, at this time or less, the chemical crosslinking is predominant over the photodegradation, producing an increase in the modules, while with 20 min the photodegradation is such that the modules suffer a significant reduction.

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UV Dose Governs UV-Polymerized Polyacrylamide Hydrogel Modulus

Cited by 22 publications

References 26 publications

On-chip protein separation with single-molecule resolution

On-chip protein separation with single-molecule resolution

Preparation of conductive composite hydrogels from carboxymethyl cellulose and polyaniline with a nontoxic crosslinking agent

Curing of Cellulose Hydrogels by UV Radiation for Mechanical Reinforcement

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