UV-C irradiation delays mitotic progression by recruiting Mps1 to kinetochores

Zhang, Xiaojuan; Ling, Youguo; Wang, Wenjun; Zhang, Yanhong; Ma, Qingjun; Tan, Pingping; Song, Ting; Wei, Congwen; Li, Ping; Liu, Xuedong; Runlin, Z.; Zhong, Hui; Cao, Cheng; Xu, Quanbin

doi:10.4161/cc.24403

Cited by 4 publications

(3 citation statements)

References 58 publications

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“…In insects, UV radiation on kinetochores at anaphase I in spermatocytes delays or inhibits the movement of homologous chromosomes to cell polars (Ilagan and Forer, 1997; Wong and Forer, 2004). Similar effect has been recorded in UV-C-radiated mitotic cells in mammals (Zhang et al, 2013). Specifically, Zhang et al reported that UV-C enhances the recruitment of the spindle checkpoint proteins MPS1 and MAD2 and the Aurora B kinase, which activates the spindle assembly checkpoint (SAC) and thus delays the segregation of homologous chromosomes (Zhang et al, 2013).…”

Section: Discussionsupporting

confidence: 85%

“…Similar effect has been recorded in UV-C-radiated mitotic cells in mammals (Zhang et al, 2013). Specifically, Zhang et al reported that UV-C enhances the recruitment of the spindle checkpoint proteins MPS1 and MAD2 and the Aurora B kinase, which activates the spindle assembly checkpoint (SAC) and thus delays the segregation of homologous chromosomes (Zhang et al, 2013). We speculate that the effect of UV-C on SAC activation might be conserved between mitosis and meiosis and across species, which explains the significantly rescued abnormal chromosome interactions in UV-C-radiated cenh3 (Fig.…”

Section: Discussionsupporting

confidence: 85%

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Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants

Fu,

Zhong,

Zhao

et al. 2024

Preprint

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Ultraviolet (UV) radiation influences development and genome stability in organisms; however, its impacts on meiosis, a special cell division essential for the delivery of genetic information over generations in eukaryotes, remain not yet elucidated. In this study, we demonstrate that UV attenuates the centromere-mediated meiotic chromosome stability and induces unreduced gametes inArabidopsis thaliana. We show that UV reduces crossover (CO) rate but does not interfere with meiotic chromosome integrity. Functional centromere-specific histone 3 (CENH3) is required for the obligate CO formation, and plays a role in protection of homolog synapsis and sister-chromatid cohesion under UV stress. Moreover, UV specifically alters the orientation and organization of spindles and phragmoplasts at meiosis II, resulting in meiotic restitution and unreduced gametes. Further, we determine that UV-induced meiotic restitution does not rely on the UV Resistance Locus8-mediated UV perception and the Tapetal Development and Function1- and Aborted Microspores-dependent tapetum development, but occurs possibly via impacted JASON function and downregulated Parallel Spindle1. This study sheds light on the impacts of UV on meiotic genome stability and gametophytic ploidy consistency, which thus may influence genome evolution in flowering plants.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 85%

Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants

Fu,

Zhong,

Zhao

et al. 2024

Preprint

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show abstract

“…The SAC signaling converges on Cdc20, a key activator of the ubiquitin E3 ligase anaphase-promoting complex/cyclosome (APC/C) that targets cyclin B and securin for proteolysis 27 28 . It has been reported that UV-C exposure can delay mitotic progression through an alternative mechanism to DNA damage response 29 . The induction of many mitotic genes following UV exposure may result in the delay of mitotic progression via activation of the SAC.…”

Section: Discussionmentioning

confidence: 99%

Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C

Takeuchi¹,

Matsuda²,

Ono³

et al. 2016

Sci Rep

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Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5 J/m2) and harvested at 4 or 12 h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (≥2-fold difference relative to the control) is higher in the sample irradiated with high dose of UV, suggesting that gene expression was UV dose-dependent. Pathway analysis on the upregulated genes at 12 h indicates that the expression of some cell cycle-related genes was predominantly induced irrespective of UV-dose. Interestingly, almost all the genes with significant altered expression were cell cycle-related genes designated as ‘Mitotic Genes’, which function in the spindle assembly checkpoint. Therefore, even a low dose of UV could affect the transcriptional profile.

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Overexpression of Mps1 in colon cancer cells attenuates the spindle assembly checkpoint and increases aneuploidy

Ling

Zhang

Bai³

et al. 2014

Biochemical and Biophysical Research Communications

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UV-C irradiation delays mitotic progression by recruiting Mps1 to kinetochores

Cited by 4 publications

References 58 publications

Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants

Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants

Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C

Overexpression of Mps1 in colon cancer cells attenuates the spindle assembly checkpoint and increases aneuploidy

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