UV-C Adaptation of Shigella: Morphological, Outer Membrane Proteins, Secreted Proteins, and Lipopolysaccharides Effects

Chourabi, Kalthoum; Campoy, Susana; Rodríguez, J; Kloula, Salma; Landoulsi, Ahmed; Chatti, Abdelwaheb

doi:10.1007/s00284-017-1311-1

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…Failure of energy dependent membrane pumps to control ion gradients and pore formation may result in an influx of water and dilution of cytoplasm. Similar morphological changes were reported for Shigella flexneri after UVC treatment [ 67 ]. The different degrees of swelling observed may also reflect differences in the reaction of LCV and SCV which differ in membrane structure [ 68 ].…”

Section: Discussionsupporting

confidence: 85%

Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen

Mertens-Scholz,

Moawad,

Liebler-Tenorio

et al. 2024

BMC Microbiol

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Q fever, a worldwide-occurring zoonotic disease, can cause economic losses for public and veterinary health systems. Vaccines are not yet available worldwide and currently under development. In this regard, it is important to produce a whole cell antigen, with preserved structural and antigenic properties and free of chemical modifications. Thus, inactivation of Coxiella burnetii with ultraviolet light C (UVC) was evaluated. C. burnetii Nine Mile phase I (NMI) and phase II (NMII) were exposed to decreasing intensities in a time-dependent manner and viability was tested by rescue cultivation in axenic medium or cell culture. Effects on the cell structure were visualized by transmission electron microscopy and antigenicity of UVC-treated NMI was studied by immunization of rabbits. NMI and NMII were inactivated at UVC intensities of 250 µW/cm2 for 5 min or 100 µW/cm2 for 20 min. Reactivation by DNA repair was considered to be unlikely. No morphological changes were observed directly after UVC inactivation by transmission electron microscopy, but severe swelling and membrane degradation of bacteria with increasing severity occurred after 24 and 48 h. Immunization of rabbits resulted in a pronounced antibody response. UVC inactivation of C. burnetii resulted in a structural preserved, safe whole cell antigen and might be useful as antigen for diagnostic purposes or as vaccine candidate.

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Section: Discussionsupporting

confidence: 85%

Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen

Mertens-Scholz,

Moawad,

Liebler-Tenorio

et al. 2024

BMC Microbiol

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“…4 ). The reason for this drastic shift of L. monocytogenes in dot plots of the flow cytometric analysis remains unknown and might have been caused by heavy alteration of lipid and protein compositions in the cell membrane structure or destruction of DNA structure affecting permeability/binding strength of fluorescence dye in L. monocytogenes [ 35 , 38 - 40 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of UV-C Irradiation on Bacterial Disinfection in a Drinking Water Purification System

Kim

Yoon

Lee

et al. 2022

J. Microbiol. Biotechnol.

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The supply of microbiological risk-free water is essential to keep food safety and public hygiene. And removal, inactivation, and destruction of microorganisms in drinking water are key for ensuring safety in the food industry. Ultraviolet-C (UV-C) irradiation is an attractive method for efficient disinfection of water without generating toxicity and adversely affecting human health. In this study, the disinfection efficiencies of UV-C irradiation on Shigella flexneri (Gram negative) and Listeria monocytogenes (Gram positive) at various concentrations in drinking water were evaluated using a water purifier. Their morphological and physiological characteristics after UV-C irradiation were observed using fluorescence microscopy and flow cytometry combined with live/dead staining. UV-C irradiation (254 nm wavelength, irradiation dose: 40 mJ/cm 2 ) at a water flow velocity of 3.4 L/min showed disinfection ability on both bacteria up to 10 8 CFU/4 L. And flow cytometric analysis showed different physiological shift between S. flexneri and L. monocytogenes after UV-C irradiation, but no significant shift of morphology in both bacteria. In addition, each bacterium revealed different characteristics with time-course observation after UV-C irradiation: L. monocytogenes dramatically changed its physiological feature and seemed to reach maximum damage at 4 h and then recovered, whereas S. flexneri seemed to gradually die over time. This study revealed that UV-C irradiation of water purifiers is effective in disinfecting microbial contaminants in drinking water and provides basic information on bacterial features/responses after UV-C irradiation.

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“…Usually, UV is detrimental for microorganisms and even fatal, especially when UV-C induces bacterial death by targeting DNA ( Dai et al, 2012 ). Bacterial exposure to sublethal UV doses leads to membrane PL bilayer rearrangement and pore formation ( Smith et al, 2009 ), inducing changes in the amounts and composition of FAs ( Khefacha et al, 2015 ) and affecting LPSs ( Chourabi et al, 2017 ). These modifications caused by UV lead to altered membrane permeability ( Santos et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station

et al. 2020

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Outer membrane vesicles (OMVs), produced by nonpathogenic Gram-negative bacteria, have potentially useful biotechnological applications in extraterrestrial extreme environments. However, their biological effects under the impact of various stressors have to be elucidated for safety reasons. In the spaceflight experiment, model biofilm kombucha microbial community (KMC) samples, in which Komagataeibacter intermedius was a dominant community-member, were exposed under simulated Martian factors (i.e., pressure, atmosphere, and UV-illumination) outside the International Space Station (ISS) for 1.5 years. In this study, we have determined that OMVs from post-flight K. intermedius displayed changes in membrane composition, depending on the location of the samples and some other factors. Membrane lipids such as sterols, fatty acids (FAs), and phospholipids (PLs) were modulated under the Mars-like stressors, and saturated FAs, as well as both short-chain saturated and trans FAs, appeared in the membranes of OMVs shed by both post-UV-illuminated and "dark" bacteria. The relative content of zwitterionic and anionic PLs changed, producing a change in surface properties of outer membranes, thereby resulting in a loss of interaction capability with polynucleotides. The changed composition of membranes promoted a bigger OMV size, which correlated with changes of OMV fitness. Biochemical characterization of the membrane-associated enzymes revealed an increase in their activity (DNAse, dehydrogenase) compared to wild type. Other functional membraneassociated capabilities of OMVs (e.g., proton accumulation, interaction with linear DNA, or synaptosomes) were also altered after exposure to the spaceflight stressors. Despite alterations in membranes, vesicles did not acquire endotoxicity, cytotoxicity, and neurotoxicity.

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UV-C Adaptation of Shigella: Morphological, Outer Membrane Proteins, Secreted Proteins, and Lipopolysaccharides Effects

Cited by 6 publications

References 43 publications

Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen

Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen

Impact of UV-C Irradiation on Bacterial Disinfection in a Drinking Water Purification System

Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station

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