UV-B stress induced metabolic rearrangements explored with comparative proteomics in three Anabaena species

Shrivastava, Alok Kumar; Chatterjee, Antra; Yadav, Shivam; Singh, Prashant; Singh, Shilpi; Rai, L.C.

doi:10.1016/j.jprot.2015.05.014

Cited by 47 publications

(16 citation statements)

References 40 publications

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“…In many cyanobacteria, cyanophycin is synthesized and accumulated upon desiccation as a dynamic reservoir of nitrogen. It can be rapidly converted into arginine by sequential degradation through cyanophycinase and isoaspartyl dipeptidase under hydration and UV‐B stress (Rajeev et al, ; Shrivastava et al, ). Based on the available KEGG annotated metabolic pathways and the newly identified ornithine–ammonia cycle (Zhang et al, ), ornithine can be synthesized by degradation of cyanophycin, followed by conversion of arginine to ornithine and ammonia by arginine dihydrolase (Fig.…”

Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic insights into the survival of the subaerial cyanobacterium Nostoc flagelliforme in arid and exposed habitats

Shang

Chen

Hou

et al. 2019

Environmental Microbiology

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Summary The cyanobacterium Nostoc flagelliforme is an extremophile that thrives under extraordinary desiccation and ultraviolet (UV) radiation conditions. To investigate its survival strategies, we performed whole‐genome sequencing of N. flagelliforme CCNUN1 and transcriptional profiling of its field populations upon rehydration in BG11 medium. The genome of N. flagelliforme is 10.23 Mb in size and contains 10 825 predicted protein‐encoding genes, making it one of the largest complete genomes of cyanobacteria reported to date. Comparative genomics analysis among 20 cyanobacterial strains revealed that genes related to DNA replication, recombination and repair had disproportionately high contributions to the genome expansion. The ability of N. flagelliforme to thrive under extreme abiotic stresses is supported by the acquisition of genes involved in the protection of photosynthetic apparatus, the formation of monounsaturated fatty acids, responses to UV radiation, and a peculiar role of ornithine metabolism. Transcriptome analysis revealed a distinct acclimation strategy to rehydration, including the strong constitutive expression of genes encoding photosystem I assembly factors and the involvement of post‐transcriptional control mechanisms of photosynthetic resuscitation. Our results provide insights into the adaptive mechanisms of subaerial cyanobacteria in their harsh habitats and have important implications to understand the evolutionary transition of cyanobacteria from aquatic environments to terrestrial ecosystems.

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Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic insights into the survival of the subaerial cyanobacterium Nostoc flagelliforme in arid and exposed habitats

Shang

Chen

Hou

et al. 2019

Environmental Microbiology

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“…To study the stress response of AKR17A1, Anabaena 7120 was subjected to different abiotic stresses such as butachlor, cadmium, UV-B, salt, heat, drought and arsenic. Our previous proteomic studies showed increased accumulation of AKR under butachlor [ 1 ], Cd [ 35 ], UV-B [ 34 ] and salt stress [ 33 ] which is also reflected in quantitative PCR analysis of AKR17A1 displaying 3.8, 4.1, 4.2 and 2.2 fold induction respectively. In line with the above results, the transcript level for AKR17A1 was also elevated upto 4.2, 2.6 and 3.1 fold under heat, drought and arsenic stress respectively ( Fig 3 and Table A in S3 File ).…”

Section: Resultsmentioning

confidence: 99%

“…Positive role of AKR in detoxification of reactive carbonyl species (RCS) produced under oxidative stress, such as methylglyoxal, HNE and TBARS has also been witnessed [ 29 , 30 ] and linked with its ability to confer tolerance against abiotic stresses [ 27 , 31 , 32 ]. A few recent proteome based studies in cyanobacteria also demonstrated enhanced accumulation of Anabaena AKR under a variety of stresses such as butachlor [ 1 ], salt [ 33 ], UV-B [ 34 ] and cadmium [ 35 ] hence indicating its physiological role in abiotic stress management. However, in diazotrophic cyanobacteria these important gene family candidates have never been investigated.…”

Section: Introductionmentioning

confidence: 99%

A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli

et al. 2015

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Present study deals with the identification of a novel aldo/keto reductase, AKR17A1 from Anabaena sp. PCC7120 and adds on as 17th family of AKR superfamily drawn from a wide variety of organisms. AKR17A1 shares many characteristics of a typical AKR such as— (i) conferring tolerance to multiple stresses like heat, UV-B, and cadmium, (ii) excellent activity towards known AKR substrates (isatin and 2-nitrobenzaldehyde), and (iii) obligate dependence on NADPH as a cofactor for enzyme activity. The most novel attribute of AKR17A1, first reported in this study, is its capability to metabolize butachlor, a persistent rice field herbicide that adversely affects agro-ecosystem and non-target organisms. The AKR17A1 catalyzed- degradation of butachlor resulted into formation of 1,2-benzene dicarboxylic acid and 2,6 bis (1,1, dimethylethyl) 4,-methyl phenol as the major products confirmed by GC-MS analysis.

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“…Anabaena species under HUV-B [12]. Proteomic study indicated that proteins related to synthesis of secondary metabolites such as caffeoylquinic acids and iridoids were increased in leaves of Lonicera japonica Thunb.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and proteomic analyses of leaves from Clematis terniflora DC. under high level of ultraviolet-B irradiation followed by dark treatment

Yang

Guan

Tian

et al. 2017

Journal of Proteomics

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This study reported response mechanism in leaves of Clematis terniflora DC. under high level of UV-B irradiation followed by dark treatment using transcriptomic and proteomic techniques. Transcriptomic analysis revealed that the number of genes related to secondary metabolism was increased by >2 times. Phenylalanine ammonia-lyase, 4-Coumarate: CoA ligase, chalcone synthase, isoflavone reductase homolog, and cinnamoyl-CoA reductase were significantly upregulated, which suggests that the secondary metabolism pathway related to lignins and flavonoids/isoflavonoids might be activated. Genes and proteins related to protein metabolism were largely changed in postranslational modification, ubiquitin proteasome, and ribosomal protein. NADP-dependent malic enzyme and NADP-malate dehydrogenase were activated in tricarboxylic acid cycle, which suggests that tricarboxylic acid cycle might be enhanced in leaf of C. terniflora in response to HUV-B+D.

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UV-B stress induced metabolic rearrangements explored with comparative proteomics in three Anabaena species

Cited by 47 publications

References 40 publications

Genomic and transcriptomic insights into the survival of the subaerial cyanobacterium Nostoc flagelliforme in arid and exposed habitats

Genomic and transcriptomic insights into the survival of the subaerial cyanobacterium Nostoc flagelliforme in arid and exposed habitats

A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli

Transcriptomic and proteomic analyses of leaves from Clematis terniflora DC. under high level of ultraviolet-B irradiation followed by dark treatment

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