UV‐B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development

Yu, Yang; Zhang, Libo; Chen, Ping; Liang, Tom; Li, Xuan; Liu, Hongtao

doi:10.15252/embj.2019101928

Cited by 115 publications

(107 citation statements)

References 59 publications

(117 reference statements)

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“…The expression of UVR8 is also found in roots along with stems and leaves (Rizzini et al 2011). MYB transcription factors MYB73/77 regulate lateral root growth by interacting with UVR8 in roots in presence of UV-B by inhibiting auxin signaling and hence integrating UV-B and auxin signaling pathways (Yang et al 2019).…”

Section: Light Signalingmentioning

confidence: 99%

Light signaling and UV‐B‐mediated plant growth regulation

Yadav

Singh

Lingwan

et al. 2020

JIPB

156

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Light plays an important role in plants’ growth and development throughout their life cycle. Plants alter their morphological features in response to light cues of varying intensity and quality. Dedicated photoreceptors help plants to perceive light signals of different wavelengths. Activated photoreceptors stimulate the downstream signaling cascades that lead to extensive gene expression changes responsible for physiological and developmental responses. Proteins such as ELONGATED HYPOCOTYL5 (HY5) and CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) act as important factors which modulate light‐regulated gene expression, especially during seedling development. These factors function as central regulatory intermediates not only in red, far‐red, and blue light pathways but also in the UV‐B signaling pathway. UV‐B radiation makes up only a minor fraction of sunlight, yet it imparts many positive and negative effects on plant growth. Studies on UV‐B perception, signaling, and response in plants has considerably surged in recent times. Plants have developed different strategies to use UV‐B as a developmental cue as well as to withstand high doses of UV‐B radiation. Plants’ responses to UV‐B are an integration of its cross‐talks with both environmental factors and phytohormones. This review outlines the current developments in light signaling with a major focus on UV‐B‐mediated plant growth regulation.

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Section: Light Signalingmentioning

confidence: 99%

Light signaling and UV‐B‐mediated plant growth regulation

Yadav

Singh

Lingwan

et al. 2020

JIPB

156

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“…Current models of UVR8 signalling involve the monomer binding to COP1, which results in stabilisation of HY5 protein (Favory et al, 2009;Rizzini et al, 2011;Huang et al, 2013), which in turn can stimulate downstream responses including its own transcription (Binkert et al, 2014). Signalling also involves direct interaction of UVR8 monomers with specific transcription factors to initiate downstream responses (Liang et al, 2018;Yang et al, 2018Yang et al, , 2020. However, there is no evidence that monomer photoreception is more, or less, likely to initiate signalling than dimer photoreception.…”

Section: Uvr8 Monomer Photoreception Likely Contributes To Uv-b Percementioning

confidence: 99%

“…The transcription factors BIM1 and BES1 mediate brassinosteroid (BR) signalling and stimulate extension growth; interaction with UVR8 in the nucleus reduces binding of these transcription factors to BR-responsive genes and hence promotes hypocotyl growth suppression by UV-B (Liang et al, 2018). MYB73 and MYB77 regulate genes involved in auxin-controlled responses, several of which are inhibited by UV-B (Vanhaelewyn et al, 2016;Jenkins, 2017); physical interaction of monomeric UVR8 with these transcription factors impairs their promoter binding activity and hence inhibits auxin-stimulated lateral root growth (Yang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…COP1 additionally interacts with the b-propeller core of the protein (Yin et al, 2015). Binding of COP1 and MYB73/MYB77 to UVR8 is UV-B dependent, whereas that of the RUPs and other transcription factors is not (Cloix et al, 2012;Yin et al, 2015;Liang et al, 2018;Yang et al, 2018Yang et al, , 2020. It is proposed that the UVR8-COP1 interaction is disrupted during RUP-mediated redimerisation (Heijde & Ulm, 2013).…”

Section: Introductionmentioning

confidence: 99%

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A dynamic model of UVR8 photoreceptor signalling in UV‐B‐acclimated Arabidopsis

et al. 2020

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The photoreceptor UVR8 mediates numerous photomorphogenic responses of plants to UV-B wavelengths by regulating transcription. Studies with purified UVR8 and seedlings not previously exposed to UV-B have generated a model for UVR8 action in which dimeric UVR8 rapidly monomerises in response to UV-B exposure to initiate signalling. However, the mechanism of UVR8 action in UV-B-acclimated plants growing under photoperiodic conditions, where UVR8 exists in a dimer/monomer photo-equilibrium, is poorly understood. We examined UVR8 dimer/monomer status, gene expression responses, amounts of key UVR8 signalling proteins and their interactions with UVR8 in UV-B-acclimated Arabidopsis. We show that in UV-B-acclimated plants UVR8 can mediate a response to a 15-fold increase in UV-B without any increase in abundance of UVR8 monomer. Following transfer to elevated UV-B, monomers show increased interaction with both COP1, to initiate signalling and RUP2, to maintain the photo-equilibrium when the dimer/monomer cycling rate increases. Native RUP1 is present in low abundance compared with RUP2. We present a model for UVR8 action in UV-B-acclimated plants growing in photoperiodic conditions that incorporates dimer and monomer photoreception, dimer/monomer cycling, abundance of native COP1 and RUP proteins, and interactions of the monomer population with COP1, RUP2 and potentially other proteins.

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“…In addition to HY5, the transcription factors HY5 HOMOLOG (HYH), FAR‐RED ELONGATED HYPOCOTYLS 3 (FHY3), BES1‐INTERACTING MYC‐LIKE1 (BIM1), WRKY36, and MYB DOMAIN PROTEIN 73 and 77 (MYB73/MYB77) participate in the regulation of gene expression in the UVR8 pathway (Brown and Jenkins 2008; Huang et al 2012; Liang et al 2018; Yang et al 2018; Liang et al 2019; Yang et al 2019). Interestingly, recent work has unraveled several UV‐B‐responsive genes that are not regulated by UVR8, suggesting the existence of alternative UV‐B signaling pathway(s) (O'Hara et al 2019).…”

Section: Introductionmentioning

confidence: 99%

The C‐terminal 17 amino acids of the photoreceptor UVR8 is involved in the fine‐tuning of UV‐B signaling

Dong

Yang

et al. 2020

JIPB

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Plant UV‐B responses are mediated by the photoreceptor UV RESISTANCE LOCUS 8 (UVR8). In response to UV‐B irradiation, UVR8 homodimers dissociate into monomers that bind to the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). The interaction of the C27 domain in the C‐terminal tail of UVR8 with the WD40 domain of COP1 is critical for UV‐B signaling. However, the function of the last 17 amino acids (C17) of the C‐terminus of UVR8, which are adjacent to C27, is unknown, although they are largely conserved in land plants. In this study, we established that Arabidopsis thaliana UVR8 C17 binds to full‐length UVR8, but not to COP1, and reduces COP1 binding to the remaining portion of UVR8, including C27. We hypothesized that overexpression of C17 in a wild‐type background would have a dominant negative effect on UVR8 activity; however, C17 overexpression caused strong silencing of endogenous UVR8, precluding a detailed analysis. We therefore generated YFP‐UVR8N423 transgenic lines, in which C17 was deleted, to examine C17 function indirectly. YFP‐UVR8N423 was more active than YFP‐UVR8, suggesting that C17 inhibits UV‐B signaling by attenuating binding between C27 and COP1. Our study reveals an inhibitory role for UVR8 C17 in fine‐tuning UVR8–COP1 interactions during UV‐B signaling.

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UV‐B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development

Cited by 115 publications

References 59 publications

Light signaling and UV‐B‐mediated plant growth regulation

Light signaling and UV‐B‐mediated plant growth regulation

A dynamic model of UVR8 photoreceptor signalling in UV‐B‐acclimated Arabidopsis

The C‐terminal 17 amino acids of the photoreceptor UVR8 is involved in the fine‐tuning of UV‐B signaling

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