UV-B-Induced PR-1 Accumulation Is Mediated by Active Oxygen Species.

Green, Rachel; Fluhr, Robert

doi:10.1105/tpc.7.2.203

Cited by 228 publications

(120 citation statements)

References 51 publications

(34 reference statements)

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“…From these results, we conclude that PR-1a is indeed induced by light of high fluences, in particular by continuous red light. The latter result excludes the influence of UV-B light, another factor known to stimulate PR-1 expression (Green and Fluhr, 1995). Blue light could also activate the PR-1a gene in the mutant, but with less efficiency than red light, whereas far-red light was ineffective.…”

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confidence: 80%

An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

et al. 1998

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A new mutant called psi2 (for p hytochrome si gnaling) was isolated by screening for elevated activity of a chlorophyll a / b binding protein-luciferase ( CAB2-LUC ) transgene in Arabidopsis. This mutant exhibited hypersensitive induction of CAB1 , CAB2 , and the small subunit of ribulose-1,5-bisphosphate carboxylase ( RBCS ) promoters in the very low fluence range of red light and a hypersensitive response in hypocotyl growth in continuous red light of higher fluences. In addition, at high-but not low-light fluence rates, the mutant showed light-dependent superinduction of the pathogenrelated protein gene PR-1a and developed spontaneous necrotic lesions in the absence of any pathogen. Expression of genes responding to various hormone and environmental stress pathways in the mutant was not significantly different from that of the wild type. Analysis of double mutants demonstrated that the effects of the psi2 mutation are dependent on both phytochromes phyA and phyB. The mutation is recessive and maps to the bottom of chromosome 5. Together, our results suggest that PSI2 specifically and negatively regulates both phyA and phyB phototransduction pathways. The induction of cell death by deregulated signaling pathways observed in psi2 is reminiscent of retinal degenerative diseases in animals and humans. INTRODUCTIONBecause light provides the energy source for photosynthesis, plants must adapt to changes in ambient light quantity and quality to optimize the efficiency of this important process. The results of this adaptation are apparent: almost every step of plant development, from seed germination to fruit maturation, is dependent on light. Moreover, light regulates the circadian rhythm, the adaptative size modification of tissue and organs, and the expression of photosynthetic genes and many other known and unknown genes (Terzaghi and Cashmore, 1995).To date, what we know about light information processing and signal transduction concerns the nature and function of photoreceptors. A family of photoreceptors called phytochromes has been shown to be involved in the perception of red and far-red light. Their biochemical and molecular properties and physiological roles are now well described (reviewed in Quail et al., 1995;Smith, 1995). In addition, a longpostulated UV and blue light receptor (cryptochrome) was recently characterized by Ahmad and Cashmore (1993). Mutants lacking one or several of these receptors have provided useful information concerning the role of individual photoreceptors and their interaction in transducing light signals (reviewed in Barnes et al., 1997).Much less is known concerning molecules that link light perception to gene activation. The CONSTITUTIVE PHOTO-MORPHOGENIC/DEETIOLATED/FUSCA (COP/DET/FUS) class of proteins has been suggested to play an important role in regulating the repression of light-inducible genes and the maintenance of skotomorphogenesis (Chory et al., 1989). For some of these proteins, the genes have been cloned and include COP1 (Deng et al., 1992), DET1 (Pepper et al., ...

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confidence: 80%

An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

et al. 1998

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“…Photo-oxidative and other environmental stresses lead to the regulation of antioxydant enzymes such as superoxide dismutases (Scandalios, 1997). Besides plant pathogen interactions, the synthesis of pathogenesis-related proteins is also enhanced by OS and ozone action (Kangasjä rvi et al, 1994), as well as UV irradiation (Green and Fluhr, 1995). Surprisingly little attention has been paid to the induction of heat shock proteins (HSP) by OS in plants.…”

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confidence: 99%

Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells

et al. 1998

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SummaryChanges in gene expression, by application of H 2 O 2 , O 2°-generating agents (methyl viologen, digitonin) and gamma irradiation to tomato suspension cultures, were investigated and compared to the well-described heat shock response. Two-dimensional gel protein mapping analyses gave the first indication that at least small heat shock proteins (smHSP) accumulated in response to application of H 2 O 2 and gamma irradiation, but not to O 2°-generating agents. While some proteins seemed to be induced specifically by each treatment, only part of the heat shock response was observed. On the basis of Northern hybridization experiments performed with four heterologous cDNA, corresponding to classes I-IV of pea smHSP, it could be concluded that significant amounts of class I and II smHSP mRNA are induced by H 2 O 2 and by irradiation. Taken together, these results demonstrate that in plants some HSP genes are inducible by oxidative stresses, as in micro-organisms and other eukaryotic cells. HSP22, the main stress protein that accumulates following H 2 O 2 action or gamma irradiation, was also purified. Sequence homology of amino terminal and internal sequences, and immunoreactivity with Chenopodium rubrum mitochondrial smHSP antibody, indicated that the protein belongs to the recently discovered class of plant mitochondrial smHSP. Heat shock or a mild H 2 O 2 pretreatment was also shown to lead to plant cell protection against oxidative injury. Therefore, the synthesis of these stress proteins can be considered as an adaptive mechanism in which mitochondrial protection could be essential.

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“…Salinity causes oxidative stress, and salt tolerance is related to the induction of antioxidant defenses [25]. UV irradiation can cause oxidative stress by generating ROS in plants [26], which leads to oxidative damage. Chilling stress is also involved in the ROS signaling pathway [27].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Type II Peroxiredoxin Gene from Panax ginseng C. A. Meyer

Kim¹,

Lee²,

Lee³

et al. 2010

Journal of Ginseng Research

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A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type II peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RT-PCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

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UV-B-Induced PR-1 Accumulation Is Mediated by Active Oxygen Species.

Cited by 228 publications

References 51 publications

An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals

Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells

Isolation and Characterization of a Type II Peroxiredoxin Gene from Panax ginseng C. A. Meyer

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