UV-B-induced photomorphogenesis in Arabidopsis

Li, Jigang; Yang, Li; Jin, Dan; Nezames, Cynthia D.; Terzaghi, William; Deng, Xing Wang

doi:10.1007/s13238-013-3036-7

Cited by 64 publications

(39 citation statements)

References 77 publications

(119 reference statements)

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“…with COP1 is a key event in UV-B signaling that induces photomorphogenic responses and also acclimation to UV-B (Heijde and Ulm, 2012;Li et al, 2013;Jenkins, 2014). Although previous results indicated the importance of a C-terminal 27-amino acid domain (C27), it was not clear how UV-B-mediated UVR8 monomerization results in UVR8-COP1 interaction based solely on C27 as the interaction domain, particularly as C27 alone results in constitutive interaction with COP1 in yeast (Cloix et al, 2012).…”

Section: Uv-b-dependent Interaction Of the Uv-b Photoreceptor Uvr8mentioning

confidence: 99%

“…UV RE-SISTANCE LOCUS8 (UVR8) is a unique UV-B photoreceptor that, following the absorption of UV-B photons, initiates changes in gene expression (Heijde and Ulm, 2012;Li et al, 2013;Tilbrook et al, 2013;Jenkins, 2014). Targets include genes involved in phenylpropanoid biosynthesis, resulting in the accumulation of phenolic "sunscreen" metabolites (e.g., flavonols and sinapates) and antioxidants (anthocyanins), as well as genes encoding photolyases, which are involved in DNA repair (Kliebenstein et al, 2002;Brown et al, 2005;Favory et al, 2009;Stracke et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Two Distinct Domains of the UVR8 Photoreceptor Interact with COP1 to Initiate UV-B Signaling in Arabidopsis

et al. 2015

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UV-B photon reception by the Arabidopsis thaliana homodimeric UV RESISTANCE LOCUS8 (UVR8) photoreceptor leads to its monomerization and a crucial interaction with CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). Relay of the subsequent signal regulates UV-B-induced photomorphogenesis and stress acclimation. Here, we report that two separate domains of UVR8 interact with COP1: the b-propeller domain of UVR8 mediates UV-B-dependent interaction with the WD40 repeats-based predicted b-propeller domain of COP1, whereas COP1 activity is regulated by interaction through the UVR8 C-terminal C27 domain. We show not only that the C27 domain is required for UVR8 activity but also that chemically induced expression of the C27 domain is sufficient to mimic UV-B signaling. We further show, in contrast with COP1, that the WD40 repeat proteins REPRESSOR OF UV-B PHOTOMORPHOGENESIS1 (RUP1) and RUP2 interact only with the UVR8 C27 domain. This coincides with their facilitation of UVR8 reversion to the ground state by redimerization and their potential to interact with UVR8 in a UV-B-independent manner. Collectively, our results provide insight into a key mechanism of photoreceptor-mediated signaling and its negative feedback regulation.

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Section: Uv-b-dependent Interaction Of the Uv-b Photoreceptor Uvr8mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Two Distinct Domains of the UVR8 Photoreceptor Interact with COP1 to Initiate UV-B Signaling in Arabidopsis

et al. 2015

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“…g085050 (herein referred to as Cr-COP1; previously also described as PUTATIVE LIGHT RESPONSE SIGNALING PROTEIN1 [LRS1]; Schierenbeck et al, 2015). The identification of Cr-COP1 further suggests the presence of a conserved UV-B signaling pathway similar to the known pathway in Arabidopsis (Heijde and Ulm, 2012;Li et al, 2013;Tilbrook et al, 2013;Jenkins, 2014). Cr-UVR8 and Cr-COP1 cDNAs were cloned and verified to match their respective sequence annotations.…”

Section: Structural Conservation and Uv-b Dynamics Of Chlamydomonas Uvr8mentioning

confidence: 99%

“…UV-B activation of UVR8 initiates a molecular signaling cascade leading to UV-B acclimation and the ability to tolerate constant UV-B exposure (Kliebenstein et al, 2002;Li et al, 2013;Tilbrook et al, 2013;Jenkins, 2014). Briefly, the UVR8 homodimer monomerizes upon UV-B exposure and interacts with CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) (Oravecz et al, 2006;Favory et al, 2009;Rizzini et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

UV-B Perception and Acclimation in Chlamydomonas reinhardtii

et al. 2016

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Plants perceive UV-B, an intrinsic component of sunlight, via a signaling pathway that is mediated by the photoreceptor UV RESISTANCE LOCUS8 (UVR8) and induces UV-B acclimation. To test whether similar UV-B perception mechanisms exist in the evolutionarily distant green alga Chlamydomonas reinhardtii, we identified Chlamydomonas orthologs of UVR8 and the key signaling factor CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). Cr-UVR8 shares sequence and structural similarity to Arabidopsis thaliana UVR8, has conserved tryptophan residues for UV-B photoreception, monomerizes upon UV-B exposure, and interacts with Cr-COP1 in a UV-B-dependent manner. Moreover, Cr-UVR8 can interact with At-COP1 and complement the Arabidopsis uvr8 mutant, demonstrating that it is a functional UV-B photoreceptor. Chlamydomonas shows apparent UV-B acclimation in colony survival and photosynthetic efficiency assays. UV-B exposure, at low levels that induce acclimation, led to broad changes in the Chlamydomonas transcriptome, including in genes related to photosynthesis. Impaired UV-B-induced activation in the Cr-COP1 mutant hit1 indicates that UVR8-COP1 signaling induces transcriptome changes in response to UV-B. Also, hit1 mutants are impaired in UV-B acclimation. Chlamydomonas UV-B acclimation preserved the photosystem II core proteins D1 and D2 under UV-B stress, which mitigated UV-B-induced photoinhibition. These findings highlight the early evolution of UVR8 photoreceptor signaling in the green lineage to induce UV-B acclimation and protection.

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“…UV-B radiation (UV-B; 280 to 315 nm) is a critical regulatory signal that induces photomorphogenic responses in plants (Heijde and Ulm, 2012;Li et al, 2013;Jenkins, 2014). These UV-B-induced responses are mediated by the photoreceptor UV RESISTANCE LOCUS8 (UVR8) in Arabidopsis thaliana (Rizzini et al, 2011) and include hypocotyl growth inhibition (Ballare et al, 1995;Kim et al, 1998;Favory et al, 2009), altered leaf morphogenesis (Hectors et al, 2007;Wargent et al, 2009), stomatal closure (Tossi et al, 2014), and the biosynthesis of UV light-absorbing "sunscreen" compounds (Beggs and Wellmann, 1994;Kliebenstein et al, 2002;Stracke et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

et al. 2014

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In plants subjected to UV-B radiation, responses are activated that minimize damage caused by UV-B. The bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) acts downstream of the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) and promotes UV-B-induced photomorphogenesis and acclimation. Expression of HY5 is induced by UV-B; however, the transcription factor(s) that regulate HY5 transcription in response to UV-B and the impact of UV-B on the association of HY5 with its target promoters are currently unclear. Here, we show that HY5 binding to the promoters of UV-B-responsive genes is enhanced by UV-B in a UVR8-dependent manner in Arabidopsis thaliana. In agreement, overexpression of REPRESSOR OF UV-B PHOTOMORPHOGENESIS2, a negative regulator of UVR8 function, blocks UV-B-responsive HY5 enrichment at target promoters. Moreover, we have identified a T/G-box in the HY5 promoter that is required for its UV-B responsiveness. We show that HY5 and its homolog HYH bind to the T/G HY5 -box cis-acting element and that they act redundantly in the induction of HY5 expression upon UV-B exposure. Therefore, HY5 is enriched at target promoters in response to UV-B in a UVR8 photoreceptor-dependent manner, and HY5 and HYH interact directly with a T/G-box cis-acting element of the HY5 promoter, mediating the transcriptional activation of HY5 in response to UV-B.

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UV-B-induced photomorphogenesis in Arabidopsis

Abstract: Ultraviolet-B (UV-B

Cited by 64 publications

References 77 publications

Two Distinct Domains of the UVR8 Photoreceptor Interact with COP1 to Initiate UV-B Signaling in Arabidopsis

Two Distinct Domains of the UVR8 Photoreceptor Interact with COP1 to Initiate UV-B Signaling in Arabidopsis

UV-B Perception and Acclimation in Chlamydomonas reinhardtii

UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

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