UV‐B‐induced DNA damage and expression of defence genes under UV‐B stress: tissue‐specific molecular marker analysis in leaves

Kalbin, Georgi; Hidema, Jun; Brosché, Mikael; Kumagai, Tadashi; Bornman, Janet F.; Strid, Åke

doi:10.1046/j.1365-3040.2001.00748.x

Cited by 47 publications

(37 citation statements)

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“…Whether the signaling involved in plant responses to UV is localized or systemic is controversial. Failure to detect up-regulation of defense genes in covered leaves of UV-B-irradiated pea (Pisum sativum) and tobacco plants has been reported by some investigators (Green and Fluhr, 1995;Kalbin et al, 2001). Others have shown that UV-induced changes in gene expression can occur in unexposed tissues of maize (Zea mays) and tobacco (Yalpani et al, 1994;Casati and Walbot, 2004).…”

Section: Discussionmentioning

confidence: 96%

“…UVenhanced transcription of the b-1,3-glucanase gene was photoreactivable (Kucera et al, 2003), implicating cyclobutane pyrimidine dimers (CPDs) or (6-4) photoproducts (6-4PPs) in transcription induction (Jiang et al, 1997a;Landry et al, 1997;Nakajima et al, 1998). However, no correlation was found between UV photoproduct levels and increased expression of several other pathogen defense genes (Green and Fluhr, 1995;Kalbin et al, 2001). Although the nature of the inducing signal is not yet clear, overlap in signaling pathways for pathogen resistance and UV-B responses, as well as enhanced defense gene expression, suggests UV may promote immunity to plant disease.…”

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confidence: 99%

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UV-Induced DNA Damage Promotes Resistance to the Biotrophic Pathogen Hyaloperonospora parasitica in Arabidopsis

et al. 2008

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Plant innate immunity to pathogenic microorganisms is activated in response to recognition of extracellular or intracellular pathogen molecules by transmembrane receptors or resistance proteins, respectively. The defense signaling pathways share components with those involved in plant responses to UV radiation, which can induce expression of plant genes important for pathogen resistance. Such intriguing links suggest that UV treatment might activate resistance to pathogens in normally susceptible host plants. Here, we demonstrate that pre-inoculative UV (254 nm) irradiation of Arabidopsis (Arabidopsis thaliana) susceptible to infection by the biotrophic oomycete Hyaloperonospora parasitica, the causative agent of downy mildew, induces dose-and timedependent resistance to the pathogen detectable up to 7 d after UVexposure. Limiting repair of UV photoproducts by postirradiation incubation in the dark, or mutational inactivation of cyclobutane pyrimidine dimer photolyase, (6-4) photoproduct photolyase, or nucleotide excision repair increased the magnitude of UV-induced pathogen resistance. In the absence of treatment with 254-nm UV, plant nucleotide excision repair mutants also defective for cyclobutane pyrimidine dimer or (6-4) photoproduct photolyase displayed resistance to H. parasitica, partially attributable to short wavelength UV-B (280-320 nm) radiation emitted by incubator lights. These results indicate UV irradiation can initiate the development of resistance to H. parasitica in plants normally susceptible to the pathogen and point to a key role for UV-induced DNA damage. They also suggest UV treatment can circumvent the requirement for recognition of H. parasitica molecules by Arabidopsis proteins to activate an immune response.

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Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

UV-Induced DNA Damage Promotes Resistance to the Biotrophic Pathogen Hyaloperonospora parasitica in Arabidopsis

et al. 2008

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“…However, action spectra of UV-B responses in plants revealed their maximal stimulation between 290 and 310 nm, whereas wavelengths below 290 nm inhibited these responses (Herrlich et al, 1997). In addition, a lack of correlation between the increase of DNA damage (finally caused by UV-B impinging on DNA) and UV-Belicited changes in transcript profiles contradicts the theory that damaged DNA serves as a UV-B receptor Frohnmeyer et al, 1999;Kalbin et al, 2001).…”

Section: Uv-b Signal Perceptionmentioning

confidence: 99%

Ultraviolet-B Radiation-Mediated Responses in Plants. Balancing Damage and Protection

2003

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“…Based on evidence from studies in other organisms, some plant responses to UV-B are thought to be initiated by signals derived from UV-B-induced DNA damage (Beggs and Wellmann, 1994). However, spectral considerations (Ballaré et al, 1995;A.-H.-Mackerness et al, 1996;Frohnmeyer et al, 1999), acclimation experiments (Kalbin et al, 2001), and studies with DNA repair mutants (Boccalandro et al, 2001) have suggested that at least some responses to UV-B are triggered by activation of other molecular targets besides DNA. In animal cells, activation of membrane receptors by UV-B is thought to be the initial step in the chain of molecular events that leads to the generation of some cellular responses to UV-B, such as apoptosis induced by exposures to high UV-B doses.…”

Section: Signaling Interactions Between Solar Uv-b-and Herbivore-indumentioning

confidence: 99%

Convergent Responses to Stress. Solar Ultraviolet-B Radiation and Manduca sexta Herbivory Elicit Overlapping Transcriptional Responses in Field-Grown Plants of Nicotiana longiflora

et al. 2003

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The effects of solar ultraviolet (UV)-B (280-315 nm) on plants have been studied intensively over the last 2 decades in connection with research on the biological impacts of stratospheric ozone depletion. However, the molecular mechanisms that mediate plant responses to solar (ambient) UV-B and their interactions with response mechanisms activated by other stressors remain for the most part unclear. Using a microarray enriched in wound-and insect-responsive sequences, we examined expression responses of 241 genes to ambient UV-B in field-grown plants of Nicotiana longiflora Cav. Approximately 20% of the sequences represented on the array showed differential expression in response to solar UV-B. The expression responses to UV-B had parallels with those elicited by simulated Manduca sexta herbivory. The most obvious similarities were: (a) down-regulation of several photosynthesis-related genes, and (b) up-regulation of genes involved in fatty acid metabolism and oxylipin biosynthesis such as HPL (hydroperoxide lyase), ␣-DIOX (alpha-dioxygenase), LOX (13-lipoxygenase), and AOS (allene oxide synthase). Genes encoding a WRKY transcription factor, a ferredoxin-dependent glutamate-synthase, and several other insect-responsive genes of unknown function were also similarly regulated by UV-B and insect herbivory treatments. Our results suggest that UV-B and caterpillar herbivory activate common regulatory elements and provide a platform for understanding the mechanisms of UV-B impacts on insect herbivory that have been documented in recent field studies.UV-B induces multiple responses in terrestrial plants. These responses have been studied using a variety of experimental approaches, from controlledenvironment experiments to large-scale field trials, and covering a broad spectrum of scales, from molecular to ecosystem level processes. Controlledenvironment experiments have contributed most of the information presently available on responses at the molecular level. However, this type of experiment, particularly those that are based on the use of heavily unbalanced light sources (i.e. with unnaturally high ratios of UV-B to photosynthetically active radiation [PAR] or UV-B to UV-A [315-400 nm]), are frequently criticized on the grounds that they lack functional and ecological realism (Fiscus and Booker, 1995;Caldwell and Flint, 1997). It has been shown clearly that treatments with high UV-B to PAR ratios, which are the norm in controlled-environment exposures, tend to result in greatly exaggerated effects of UV-B on photosynthesis and growth inhibition. Because these effects have been hard to duplicate under field conditions, it is unclear to what extent the molecular and mechanistic data that have been produced under spectrally unbalanced conditions (which commonly involve PAR levels lower than 1:10 of full sunlight) contribute to our understanding of the normal physiological responses of plants to ambient UV-B stress. In fact, it has been clearly demonstrated that the level of PAR strongly modulates the effect of UV-B on...

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UV‐B‐induced DNA damage and expression of defence genes under UV‐B stress: tissue‐specific molecular marker analysis in leaves

Cited by 47 publications

References 46 publications

UV-Induced DNA Damage Promotes Resistance to the Biotrophic Pathogen Hyaloperonospora parasitica in Arabidopsis

UV-Induced DNA Damage Promotes Resistance to the Biotrophic Pathogen Hyaloperonospora parasitica in Arabidopsis

Ultraviolet-B Radiation-Mediated Responses in Plants. Balancing Damage and Protection

Convergent Responses to Stress. Solar Ultraviolet-B Radiation and Manduca sexta Herbivory Elicit Overlapping Transcriptional Responses in Field-Grown Plants of Nicotiana longiflora

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