UV-B Filter Octylmethoxycinnamate Induces Vasorelaxation by Ca2+ Channel Inhibition and Guanylyl Cyclase Activation in Human Umbilical Arteries

Lorigo, Margarida; Quintaneiro, Carla; Lemos, Manuel C.; Martinez-de-Oliveira, José; Breitenfeld, Luiza

doi:10.3390/ijms20061376

Cited by 13 publications

(13 citation statements)

References 63 publications

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“…Studies using natural products in human umbilical vessels are scarce. Studies demonstrating a vasorelaxant effect in HUAs include Campos et al [34], which used the polyphenol rich fraction (chlorogenic acid, isoorientin and swertiajaponin) from the Cymbopogon citratus extract; Lorigo et al [35], which used octyl methoxycinnamate, an organic compound used in the manufacture of sunscreens; Speroni et al [36], which used genistein, a natural phytoestrogen belonging to the isoflavone group; and Massaro et al [37], which used bee propolis, which induced a vasorelaxant effect in HUAs in the presence of K + (60 mM).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, endogenous substances, such as progesterone and 5β-pregnanediol, have shown that VOCC inhibition is the main vascular relaxation route stimulated by these hormones in HUA vessels [33]. Other aforementioned natural products, such as octyl methoxycinnamate [35] and genistein [36], have also demonstrated a relaxant effect on HUAs through the inhibition of VOCCs.…”

Section: Discussionmentioning

confidence: 99%

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Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

Silva

Pereira-de-Morais

et al. 2020

Molecules

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Carveol is a monoterpene present in the structure of many plant products. It has a variety of biological activities: antioxidant, anticancer and vasorelaxation. However, studies investigating the effect of monoterpenoids on human vessels have not yet been described. Thus, the present study aimed to characterize the effect of (−)-carveol on human umbilical arteries (HUAs). HUA ring preparations were isolated and subjected to isometric tension recordings of umbilical artery smooth muscle contractions. (−)-Carveol exhibited a significant vasorelaxant effect on KCl and 5-HT-induced contractions, obtaining EC50 values of 344.25 ± 8.4 and 175.82 ± 4.05 µM, respectively. The participation of calcium channels in the relaxation produced by (−)-carveol was analyzed using vessels pre-incubated with (−)-carveol (2000 µM) in a calcium-free medium, where the induction of contractions was abolished. The vasorelaxant effect of (−)-carveol on HUAs was reduced by tetraethylammonium (TEA), which increased the (−)-carveol EC50 to 484.87 ± 6.55 µM. The present study revealed that (−)-carveol possesses a vasorelaxant activity in HUAs, which was dependent on the opening of calcium and potassium channels. These results pave the way for further studies involving the use of monoterpenoids for the vasodilatation of HUAs. These molecules have the potential to treat diseases such as pre-eclampsia, which is characterized by resistance in umbilical arteries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

Silva

Pereira-de-Morais

et al. 2020

Molecules

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“…The tension recordings of HUA were performed according to our previous work [ 35 ]. Following the equilibration period and before starting the experiments, rings were transiently challenged with a supramaximal concentration of 5-HT (1 μmol/L) to assess the functional state of each vessel.…”

Section: Methodsmentioning

confidence: 99%

“…Each ring was precontracted using 5-HT (1 μmol/L), histamine (His, 10 μmol/L) or potassium chloride (KCl, 60 mmol/L). Cumulative pharmacologic concentrations (0.001–50 μmol/L) were used according to the previous studies [ 35 , 36 ]. Control experiments were performed using solvent (ethanol) at same % used to dissolve the OMC.…”

Section: Methodsmentioning

confidence: 99%

UV-B Filter Octylmethoxycinnamate Alters the Vascular Contractility Patterns in Pregnant Women with Hypothyroidism

2021

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Increasing evidence relating the exposure and/or bioaccumulation of endocrine-disrupting compounds (EDCs) with cardiovascular system are arising. Octylmethoxycinnamate (OMC) is the most widely used UV-B filter and as EDC interacts with TH receptors. However, their effects on thyroid diseases during pregnancy remain unknown. The purpose of this work was to assess the short- and long-term effects of OMC on arterial tonus of pregnant women with hypothyroidism. To elucidate this, human umbilical artery (HUA) rings without endothelium were used to explore the vascular effects of OMC by arterial and cellular experiments. The binding energy and the modes of interaction of the OMC into the active center of the TSHR and THRα were analyzed by molecular docking studies. Our results indicated that OMC altered the contractility patterns of HUA contracted with serotonin, histamine and KCl, possibly due to an interference with serotonin and histamine receptors or an involvement of the Ca2+ channels. The molecular docking analysis show that OMC compete with T3 for the binding center of THRα. Taken together, these findings pointed out to alterations in HUA reactivity as result of OMC-exposure, which may be involved in the development and increased risk of cardiovascular diseases.

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“…Octylmethoxycinnamate (OMC, also known by ethylhexylmethoxycinnamate or octinoxate) is the world's most widely used UV-B filter in sunscreens. Due to its potential risks to damage DNA (Duale et al 2010;Necasova et al 2017;Sharma et al 2017) associated to the demonstrated endocrine disruptor effects (Lorigo et al 2019;Schlumpf et al 2001;Schlumpf et al 2004) in humans, the safety of this organic filter is a current concern for human health. Antioxidants have a potential to neutralize the UV-induced free radicals (Addor 2017; Dunaway et al 2018), so their use as UV filter stabilizers is a novel photoprotective strategy (Galanakis et al 2018;Wang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Antioxidants as stabilizers of UV filters: an example for the UV-B filter octylmethoxycinnamate

Lorigo

2019

biomed dermatol

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Background: Sunlight is one of the main harmful exogenous factors that induce the reactive oxygen species formation. The human skin is the first line of photoprotection against harmful exogenous factors, such as UV radiations. The topical application of sunscreens, containing UV-B filters, is widely used to protect against UV-induced damage. Octylmethoxycinnamate is the world's most widely used UV-B filter in sunscreens. However, recent studies have demonstrated that this substance is an endocrine disruptor compound and with potential to damage DNA. Thus, the safety of this organic filter is a current concern for human health, and it was urgent to develop new photoprotective strategies. In this sense, due to the potential to neutralize the UV-induced free radicals, the use of antioxidants as UV filter stabilizers presented as a novel promising strategy. Research: The purpose of this review was to assess the use of antioxidants as stabilizers for UV-B filter octylmethoxycinnamate. For this, we discuss the chemical and physical characteristics of UV-B filter octylmethoxycinnamate, emphasizing the stability, photostability, and reactivity of this UV filter. The use of antioxidants in sunscreens will also be addressed, from a perspective of the main characteristics that allowed their use in sunscreen formulations. Then, the concomitant use of both was described from a historical and physical chemical perspective, always emphasizing the advantages and disadvantages of this association. Conclusions: The combination of antioxidants with UV-B filter octylmethoxycinnamate in appropriated formulations represents a viable strategy to protect the human skin against UV-induced damage.

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UV-B Filter Octylmethoxycinnamate Induces Vasorelaxation by Ca2+ Channel Inhibition and Guanylyl Cyclase Activation in Human Umbilical Arteries

Cited by 13 publications

References 63 publications

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

UV-B Filter Octylmethoxycinnamate Alters the Vascular Contractility Patterns in Pregnant Women with Hypothyroidism

Antioxidants as stabilizers of UV filters: an example for the UV-B filter octylmethoxycinnamate

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