UV-activated multilayer nanomatrix provides one-step tunable carbohydrate structural characterization in MALDI-MS

Abstract

Our work highlights DHB@MNP-induced pseudo-MS/MS for oligosaccharide characterization, with some insights on this nanoparticle-mediated energy transfer dynamics.

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

“…At low concentrations, UV laser‐excited DHB@MNP provides enhanced soft ionization of intact molecular ions, whereas excess energy absorption and dissipation from high DHB@MNP concentrations caused extensive but controlled fragmentation. The method has been used to distinguish a series of isomeric trisaccharide and tetrasaccharide analytes (Obena et al, 2015).…”

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Mechanistic Studies and Methods for Improving the Structural Identification of Carbohydrates

“…116) Nanoparticles (NPs), such as gold, silver, titanium dioxide, iron oxide (Fe 3 O 4 ), zinc oxide, and manganese oxide, have been utilized for the analysis of carbohydrates because they enhance ionization e ciency by releasing ionization agents. [117][118][119][120][121] A er absorbing laser energy, NPs also convert photon energy to thermal energy to facilitate material desorption. Charge and energy transfer from NPs to analytes triggers ionization/fragmentation and desorption of carbohydrates.…”

“…Charge and energy transfer from NPs to analytes triggers ionization/fragmentation and desorption of carbohydrates. 117,122) Furthermore, functionalization of NPs with conventional organic matrices have been reported to serve as new matrix materials to preserve the intact glycan as well as the generation of extensive cleavages in MALDI. 123) More details about methods on the structural identi cation of carbohydrates are reviewed in the following section.…”

Functionalized HgTe nanoparticles promote laser-induced solid phase ionization/dissociation for comprehensive glycan sequencing

“…A material with lower threshold laser energy for producing photoelectrons is expected to generate fragmentation with higher efficiency, such as the iron oxide NP which generates photoelectrons and triggers the fragmentation processes in the solid phase. 20,21 In addition, the thermal energy released from NPs after laser excitation not only facilitates material desorption, but also fuels the fragmentation processes. The thermal energy of NPs changes the surface temperature and emits IR radiation.…”

“…15 Recently, there have also been some attempts to promote analyte dissociation by employing glutathionecapped iron oxide NPs or by UV-absorbing magnetic NPs as a desorption-fragmentation element. 20,21 Compared to the use of a conventional DHB matrix, oligosaccharide isomers and simple mixtures were distinguished unambiguously by their accurate mass and characteristic fingerprint fragment ions employing DHB@MNP. 21 Given their potential for structure characterization, these studies inspire a quest to further explore the NP's competency as a tool to delineate a molecule's fine structure, which gives full and detailed information on the structure-function relationship.…”