utPCR: A Strategy for Highly Specific and Absolutely Quantitative Detection of Single Molecules within Only Minutes
Rui Wang,
Ying Liu,
Shuaiwei Chen
et al.
Abstract:Bloodstream infection is a major health problem worldwide, with extremely high mortality. Detecting infection in the early stage is challenging due to the extremely low concentration of bacteria in the blood. Digital PCR provides unparalleled sensitivity and can achieve absolute quantification, but it is time-consuming. Moreover, the presence of unavoidable background signals in negative controls poses a significant challenge for single-molecule detection. Here, we propose a novel strategy called “Ultrafast fl… Show more
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